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Details on Person UniProt:P15927 RPA2

Class:Id	ReferenceGeneProduct:99865
_chainChangeLog	chain:1-270 added on Fri February 6 2015
_displayName	UniProt:P15927 RPA2
_timestamp	2025-08-15 22:11:22
chain	chain:1-270
checksum	61A563EA7B34A9B1
comment	FUNCTION As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542).SUBUNIT Component of the replication protein A complex (RPA/RP-A), a heterotrimeric complex composed of RPA1, RPA2 and RPA3 (PubMed:10449415, PubMed:19116208, PubMed:2406247). Interacts with PRPF19; the PRP19-CDC5L complex is recruited to the sites of DNA repair where it ubiquitinates the replication protein A complex (RPA) (PubMed:24332808). Interacts with SERTAD3 (PubMed:10982866). Interacts with TIPIN (PubMed:17141802, PubMed:17296725). Interacts with TIMELESS (PubMed:17141802). Interacts with PPP4R2; the interaction is direct, DNA damage-dependent and mediates the recruitment of the PP4 catalytic subunit PPP4C (PubMed:20154705). Interacts (hyperphosphorylated) with RAD51 (PubMed:20154705). Interacts with SMARCAL1; the interaction is direct and mediates the recruitment to the RPA complex of SMARCAL1 (PubMed:19793861, PubMed:19793862, PubMed:19793863, PubMed:24910198). Interacts with RAD52 and XPA; those interactions are direct and associate RAD52 and XPA to the RPA complex (PubMed:11081631, PubMed:17765923, PubMed:7700386, PubMed:8702565). Interacts with FBH1 (PubMed:23319600). Interacts with ETAA1; the interaction is direct and promotes ETAA1 recruitment at stalled replication forks (PubMed:27601467, PubMed:27723717, PubMed:27723720). Interacts with RFWD3 (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657). Interacts with DDI2 (PubMed:29290612). Interacts (in unphosphorylated form via N-terminus) with EIF4EBP3; the interaction enhances EIF4EBP3-mediated inhibition of EIF4E-mediated mRNA nuclear export (PubMed:22684010). Interacts with BRIP1/FANCJ via the RPA1 subunit; following DNA damage they colocalize in foci in the nucleus (PubMed:17596542). Interacts with nuclear UNG (isoform 2); this interaction mediates UNG recruitment to RPA-coated single-stranded DNA at stalled replication forks.INTERACTION Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner.ALTERNATIVE PRODUCTS Translationally up-regulated in response to DNA damage (at protein level).PTM Differentially phosphorylated throughout the cell cycle, becoming phosphorylated at the G1-S transition and dephosphorylated in late mitosis. Mainly phosphorylated at Ser-23 and Ser-29, by cyclin A-CDK2 and cyclin B-CDK1, respectively during DNA replication and mitosis. Dephosphorylation may require the serine/threonine-protein phosphatase 4. Phosphorylation at Ser-23 and Ser-29 is a prerequisite for further phosphorylation. Becomes hyperphosphorylated on additional residues including Ser-4, Ser-8, Thr-21 and Ser-33 in response to DNA damage. Hyperphosphorylation is mediated by ATM, ATR and PRKDC. Primarily recruited to DNA repair nuclear foci as a hypophosphorylated form it undergoes subsequent hyperphosphorylation, catalyzed by ATR. Hyperphosphorylation is required for RAD51 recruitment to chromatin and efficient DNA repair. Phosphorylation at Thr-21 depends upon RFWD3 presence.PTM DNA damage-induced 'Lys-63'-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR (PubMed:24332808). Ubiquitinated by RFWD3 at stalled replication forks in response to DNA damage: ubiquitination by RFWD3 does not lead to degradation by the proteasome and promotes removal of the RPA complex from stalled replication forks, promoting homologous recombination (PubMed:26474068).SIMILARITY Belongs to the replication factor A protein 2 family.
description	recommendedName: Replication protein A 32 kDa subunit shortName: RP-A p32 alternativeName: Replication factor A protein 2 shortName: RF-A protein 2 alternativeName: Replication protein A 34 kDa subunit shortName: RP-A p34
geneName	RPA2 REPA2 RPA32 RPA34
identifier	P15927
isSequenceChanged	FALSE
keyword	3D-structure Acetylation Alternative splicing Direct protein sequencing DNA damage DNA recombination DNA repair DNA replication DNA-binding Isopeptide bond Nucleus Phosphoprotein Proteomics identification Reference proteome Ubl conjugation
modified	[InstanceEdit:9836292] Weiser, Joel, 2023-05-25 [InstanceEdit:9852000] Weiser, Joel, 2023-11-03 [InstanceEdit:9862192] Weiser, Joel, 2024-02-26 [InstanceEdit:9917590] Weiser, Joel, 2024-08-09 [InstanceEdit:9926675] Weiser, Joel, 2024-11-03 [InstanceEdit:9948485] Weiser, Joel, 2025-05-21 [InstanceEdit:9963647] Weiser, Joel, 2025-08-15
name	RPA2
referenceDatabase	[ReferenceDatabase:2] UniProt
referenceGene	[ReferenceDNASequence:8989765] ENSEMBL:ENSG00000117748 RPA2 [Homo sapiens]
secondaryIdentifier	RFA2_HUMAN Q52II0 Q5TEI9 Q5TEJ5
sequenceLength	270
species	[Species:48887] Homo sapiens
(isoformParent)	[ReferenceIsoform:245353] UniProt:P15927-2 RPA2 [Homo sapiens] [ReferenceIsoform:245354] UniProt:P15927-3 RPA2 [Homo sapiens] [ReferenceIsoform:403018] UniProt:P15927-1 RPA2 [Homo sapiens]
(referenceEntity)	[EntityWithAccessionedSequence:68457] RPA2 [nucleoplasm] [Homo sapiens] [EntityWithAccessionedSequence:5685148] p-S33-RPA2 [nucleoplasm] [Homo sapiens]
(referenceSequence)	[ModifiedResidue:5685149] O-phospho-L-serine at 33
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No pathways have been reviewed or authored by UniProt:P15927 RPA2 (99865)