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Details on Person UniProt:P23847 dppA

Class:IdReferenceGeneProduct:9927601
_chainChangeLogsignal peptide:1-28 for 9927601 added on Fri Feb 21 2025;chain:29-535 for 9927601 added on Fri Feb 21 2025
_displayNameUniProt:P23847 dppA
_timestamp2025-02-21 18:55:58
chainsignal peptide:1-28
chain:29-535
checksum3F7C4756EEA8C2F0
commentFUNCTION Part of the ABC transporter DppABCDF involved in dipeptide transport (PubMed:10537211, PubMed:1702779, PubMed:7536291). Binds dipeptides and accepts a wide range of side chains, including small neutral, bulky hydrophobic, and positively and negatively charged groups (PubMed:10537211). Tripeptides are poor substrates (PubMed:10537211). DppA alone controls the specificity of the Dpp transporter (PubMed:10537211). In addition, plays a role in chemotaxis toward peptides via interaction with the chemotaxis protein Tap (PubMed:3520334, PubMed:8563629).FUNCTION Binds heme. When a foreign outer membrane heme receptor is expressed in E.coli, DppABCDF can also transport heme and its precursor, 5-aminolevulinic acid (ALA), from the periplasm into the cytoplasm.ACTIVITY REGULATION Heme binding is inhibited by dipeptide.SUBUNIT The complex is composed of two ATP-binding proteins (DppD and DppF), two transmembrane proteins (DppB and DppC) and a solute-binding protein (DppA).SUBCELLULAR LOCATION Expression is repressed by the presence of casamino acids.DOMAIN Consists of two distinct domains (I and II) connected by two strands that presumably function as a hinge (PubMed:8527431, PubMed:8563629). Undergoes conformational change upon substrate binding (PubMed:10537211).DISRUPTION PHENOTYPE Inactivation of the gene results in the inability of a proline auxotroph to utilize Pro-Gly as a proline source (PubMed:1702779). Inactivation of the gene has no effect on iron-heme utilization, but the double mppA dppA mutant is unable to use heme as iron source (PubMed:16905647).SIMILARITY Belongs to the bacterial solute-binding protein 5 family.
created[InstanceEdit:9927596] Stephan, Ralf, 2024-11-08
descriptionrecommendedName: fullName evidence="13 14"Dipeptide-binding protein shortName evidence="14"DBP alternativeName: fullName evidence="13"Periplasmic dipeptide transport protein
geneNamedppA
b3544
JW3513
identifierP23847
isSequenceChangedFALSE
keyword3D-structure
Chemotaxis
Direct protein sequencing
Disulfide bond
Peptide transport
Periplasm
Protein transport
Reference proteome
Signal
Transport
modified[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
namedppA
referenceDatabase[ReferenceDatabase:2] UniProt
secondaryIdentifierDPPA_ECOLI
Q2M7K6
sequenceLength535
species[Species:159879] Escherichia coli
(referenceEntity)[EntityWithAccessionedSequence:9927592] DppA [periplasmic space] [Escherichia coli]
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