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Details on Person UniProt:P04545 M2-1

Class:IdReferenceGeneProduct:9828699
_chainChangeLogchain:1-194 for 9828699 added on Fri February 24 2023
_displayNameUniProt:P04545 M2-1
_timestamp2025-02-21 20:11:15
chainchain:1-194
checksumCA5B397FE2707EF9
commentFUNCTION Acts as a tetrameric transcription processivity factor that binds in a competitive manner to RNA and the phosphoprotein (P) to prevent premature termination during transcription (PubMed:19386701, PubMed:22675274). Transcription anti-terminator that enhances readthrough of intergenic junctions during viral transcription (PubMed:10364337, PubMed:27194388, PubMed:8552680, PubMed:9420254). Preferentially binds to poly(A)-rich sequences (PubMed:24434552). Plays a role in the association of the matrix protein with the nucleocapsid, which initiates assembly and budding (PubMed:18579594). Also, can activate host NF-kappa-B through association with host RELA (PubMed:15629770).SUBUNIT Homotetramer (PubMed:19386701, PubMed:29372904, PubMed:32697936). The homotetramer interacts with RNA (PubMed:22675274, PubMed:32697936). Interacts with the phosphoprotein (P); this interaction is required for protein M2-1 function, localization in host inclusion bodies (PubMed:12970453, PubMed:17098979, PubMed:19386701, PubMed:22675274, PubMed:22978633, PubMed:29489893). Formation of a complex host PP1/M2-1/P allows P to target host PP1 phosphatase to the M2-1 substrate (PubMed:29489893). Interacts with the nucleoprotein (N) (PubMed:10846068, PubMed:22675274). Interacts with the matrix protein (M); this interaction directs M localization to cytoplasmic inclusions comprising viral proteins L, N, P, and M2-1 and mediates M association with the nucleocapsid (PubMed:18579594). Interacts with host RELA (PubMed:15629770). Interacts with host PABPC1 (via C-terminus) (PubMed:31649314).INTERACTION Localizes in cytoplasmic inclusion bodies substructures called inclusion bodies associated granules (IBAGs) (PubMed:22675274, PubMed:28916773, PubMed:29489893, PubMed:31649314). Forms a layer between the matrix and nucleocapsid (PubMed:23776214, PubMed:24760890).INDUCTION Down-regulated by cyclopamine (PubMed:27194388). Expressed early in the viral replication cycle (PubMed:10846068).DOMAIN Contains a zinc-finger domain on its N-terminus essential for its anti-termination function (PubMed:24434552). Contains an oligomerization domain (PubMed:22675274). The central globular core is responsible for binding to RNA and phosphoprotein (PubMed:22675274).PTM Phosphorylated by host in infected cells (PubMed:10846068, PubMed:11711610, PubMed:29489893, PubMed:3339328). Only dephosphorylated M2-1 is competent for viral mRNA binding (PubMed:29489893). Cyclic turnover of phosphorylation-dephosphorylation of M2-1 is required for efficient viral transcription (PubMed:29489893).SIMILARITY Belongs to the pneumoviridae M2-1 protein family.
created[InstanceEdit:9828690] Orlic-Milacic, Marija, 2023-02-16
descriptionrecommendedName: Protein M2-1 alternativeName: Envelope-associated 22 kDa protein alternativeName: fullName evidence="28"Transcription antitermination factor M2-1 alternativeName: fullName evidence="29"Vp24
geneNameM2-1
identifierP04545
isSequenceChangedFALSE
keyword3D-structure
Activation of host NF-kappa-B by virus
Host cytoplasm
Host nucleus
Host-virus interaction
Metal-binding
Phosphoprotein
RNA-binding
Transcription
Transcription antitermination
Transcription regulation
Viral transcription
Virion
Zinc
Zinc-finger
modified[InstanceEdit:9829221] Weiser, Joel
[InstanceEdit:9834092] Weiser, Joel
[InstanceEdit:9841277] Weiser, Joel
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9917590] Weiser, Joel, 2024-08-09
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
nameM2-1
referenceDatabase[ReferenceDatabase:2] UniProt
secondaryIdentifierM21_HRSVA
Q77YA8
sequenceLength194
species[Species:9717528] Human respiratory syncytial virus A
(referenceEntity)[EntityWithAccessionedSequence:9828687] Nascent M2-1 [cytosol] [Human respiratory syncytial virus A]
[EntityWithAccessionedSequence:9831952] p-M2-1 [cytosol] [Human respiratory syncytial virus A]
[EntityWithAccessionedSequence:9831958] p-M2-1 [nucleoplasm] [Human respiratory syncytial virus A]
(referenceSequence)[ModifiedResidue:9831945] O-phospho-L-serine at 61
[ModifiedResidue:9832011] O-phospho-L-serine at 58
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No pathways have been reviewed or authored by UniProt:P04545 M2-1 (9828699)