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Details on Person UniProt:P41784 sctF1
| Class:Id | ReferenceGeneProduct:9687894 |
|---|---|
| _chainChangeLog | chain:1-80 for 9687894 added on Fri May 15 2020 |
| _displayName | UniProt:P41784 sctF1 |
| _timestamp | 2026-02-20 22:15:50 |
| chain | chain:1-80 |
| checksum | ED1F8967877585F8 |
| comment | FUNCTION Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:12754250, PubMed:16501225, PubMed:30242280, PubMed:33750771). PrgI/SctF1 forms the external needle filament that protrudes from the bacterial surface (PubMed:12754250, PubMed:16501225, PubMed:22699623, PubMed:30242280, PubMed:31260457, PubMed:33750771). Is probably involved in the transduction of an activating signal, thought to be mediated by the distal tip of the needle filament, to the secretion machine (PubMed:31260457). Required for invasion of epithelial cells (PubMed:17617421). Required for the secretion of the effector protein SptP (PubMed:31260457, PubMed:33750771).FUNCTION During infection, can induce innate immune responses (PubMed:24643544). The needle proteins interact with host TLR2 or TLR4, and induce signaling by NF-kappa-B and/or AP-1 (PubMed:24643544). This activation is MyD88 dependent and results in increased expression of cytokines, including TNF, IL-6 and IL-8 (PubMed:24643544).ACTIVITY REGULATION Binding of bile salts, including deoxycholate, to the PrgI:SipD interface may inhibit the T3SS function.SUBUNIT The core secretion machinery of the T3SS is composed of approximately 20 different proteins, including cytoplasmic components, a base, an export apparatus and a needle (PubMed:30107569, PubMed:30242280, PubMed:31427728). This subunit polymerizes and forms the helical needle filament (PubMed:16501225, PubMed:20543831, PubMed:22699623, PubMed:30242280, PubMed:31260457, PubMed:31427728, PubMed:33750771, PubMed:34258394, PubMed:34706941). Interacts with the needle tip protein SipD/SctA (PubMed:21829362, PubMed:34706941). Interacts with the needle adapter protein PrgJ/SctI, the secretin InvG/SctC and the minor export apparatus protein SpaP/SctR (PubMed:33750771). In vitro, the needle protomer refolds spontaneously to extend the needle from the distal end (PubMed:20543831).INTERACTION Negatively regulated by PhoP-PhoQ.DOMAIN Deletion of the last five residues prevents self-association and yields monomeric proteins that allow biophysical studies (PubMed:16501225, PubMed:17617421). Forms a right-handed helical assembly with roughly 11 subunits per two turns (PubMed:22699623, PubMed:30242280). The location of the negatively charged surfaces are radically different among the needle filament proteins PrgI, BsaL and MxiH (PubMed:17617421). In PrgI, the major negatively charged surface is not on the 'face' but instead is on the 'side' of the two-helix bundle, and only residues from helix alpha1 contribute to this negative region (PubMed:17617421). The insertion of the needle tip complex on the distal end of the filament results in significant conformational changes in PrgI/SctF (PubMed:34706941).DISRUPTION PHENOTYPE The null mutant strain is non-invasive.MISCELLANEOUS The levels of PrgI/SctF are reduced in an invA/sctV mutant, probably due to protein instability, and are increased in an invJ/sctP mutant.SIMILARITY Belongs to the SctF family. |
| created | [InstanceEdit:9687934] Varusai, Thawfeek, 2020-05-13 |
| description | recommendedName: fullName evidence="18"SPI-1 type 3 secretion system needle filament protein shortName evidence="18"T3SS-1 needle filament protein |
| geneName | sctF1 prgI STM2873 |
| identifier | P41784 |
| isSequenceChanged | FALSE |
| keyword | 3D-structure Protein transport Reference proteome Secreted Transport Virulence |
| modified | [InstanceEdit:9688885] Weiser, JD [InstanceEdit:9852000] Weiser, Joel, 2023-11-03 [InstanceEdit:9917590] Weiser, Joel, 2024-08-09 [InstanceEdit:9983091] Weiser, Joel, 2026-02-20 |
| name | sctF1 |
| referenceDatabase | [ReferenceDatabase:2] UniProt |
| secondaryIdentifier | SCTF1_SALTY |
| sequenceLength | 80 |
| species | [Species:451465] Salmonella typhimurium |
| (referenceEntity) | [EntityWithAccessionedSequence:9687880] prgI [cell outer membrane] [Salmonella typhimurium] [EntityWithAccessionedSequence:9687915] Ub-prgI [cell outer membrane] [Salmonella typhimurium] |
| (referenceSequence) | [GroupModifiedResidue:9687856] ubiquitinylated lysine (M1polyUb [cytosol]) at 66 [GroupModifiedResidue:9687865] ubiquitinylated lysine (M1polyUb [cytosol]) at 37 [GroupModifiedResidue:9687920] ubiquitinylated lysine (M1polyUb [cytosol]) at 69 [GroupModifiedResidue:9687923] ubiquitinylated lysine (M1polyUb [cytosol]) at 33 |
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No pathways have been reviewed or authored by UniProt:P41784 sctF1 (9687894)
