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Details on Person UniProt:Q9H3P2 NELFA

Class:IdReferenceGeneProduct:96389
_chainChangeLogchain:1-528 added on Fri February 6 2015
_displayNameUniProt:Q9H3P2 NELFA
_timestamp2024-11-03 20:02:05
chainchain:1-528
checksum4F33C3BC68BDC798
commentFUNCTION Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.FUNCTION (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.SUBUNIT The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE; NELFA and NELFCD form a stable subcomplex that binds to the N-terminus of NELFB (PubMed:11387440). In vitro, the NELFA:NELFCD subcomplex binds to ssDNA and ssRNA in a sequence- and structure-dependent manner (PubMed:27282391). Interacts with the RNA polymerase II complex when it is not phosphorylated by P-TEFb (PubMed:10199401).INTERACTION Ubiquitous. Expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Expressed at lower level in adult lung. Expressed in fetal brain, lung, liver and kidney.DOMAIN The HDAg-like domain is essential for transcriptional repression, and mediates the interaction with the RNA polymerase II complex.SIMILARITY Belongs to the NELF-A family.CAUTION PubMed:12612062 has shown that it is not involved in Wolf-Hirschhorn syndrome.SEQUENCE CAUTION Truncated N-terminus.SEQUENCE CAUTION Extended N-terminus.SEQUENCE CAUTION Extended N-terminus.
descriptionrecommendedName: Negative elongation factor A shortName: NELF-A alternativeName: Wolf-Hirschhorn syndrome candidate 2 protein
geneNameNELFA
WHSC2
P/OKcl.15
identifierQ9H3P2
isSequenceChangedFALSE
keyword3D-structure
Alternative splicing
Direct protein sequencing
Nucleus
Phosphoprotein
Proteomics identification
Reference proteome
Repressor
Transcription
Transcription regulation
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
nameNELFA
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:8994374] ENSEMBL:ENSG00000185049 NELFA [Homo sapiens]
secondaryIdentifierNELFA_HUMAN
A2A2T1
O95392
sequenceLength528
species[Species:48887] Homo sapiens
(isoformParent)[ReferenceIsoform:385685] UniProt:Q9H3P2-7 NELFA [Homo sapiens]
[ReferenceIsoform:414226] UniProt:Q9H3P2-1 NELFA [Homo sapiens]
(referenceEntity)[EntityWithAccessionedSequence:112426] NELFA [nucleoplasm] [Homo sapiens]
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