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Details on Person UniProt:P30307 CDC25C

Class:IdReferenceGeneProduct:95494
_chainChangeLoginitiator methionine:1 added on Fri February 6 2015;chain:2-473 added on Fri February 6 2015;initiator methionine:1 for 95494 removed on Fri Nov 03 2023;initiator methionine: for 95494 added on Fri Nov 03 2023;initiator methionine: for 95494 removed on Fri Aug 15 2025;initiator methionine:1 for 95494 added on Fri Aug 15 2025
_displayNameUniProt:P30307 CDC25C
_timestamp2025-08-15 22:09:43
chaininitiator methionine:1
chain:2-473
checksum0658A1F1B9B8996A
commentFUNCTION Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle (PubMed:8119945). When phosphorylated, highly effective in activating G2 cells into prophase (PubMed:8119945). Directly dephosphorylates CDK1 and activates its kinase activity (PubMed:8119945).CATALYTIC ACTIVITY O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphateSUBUNIT Interacts with MAPK14 and 14-3-3 proteins (PubMed:11333986). When phosphorylated on Ser-129 and/or Thr-130, interacts with PLK1 (PubMed:12595692). Interacts with MARK3/C-TAK1 (PubMed:12941695).SUBUNIT (Microbial infection) Interacts with HIV-1 Vpr; this interaction inactivates CDC25C phosphatase activity.INTERACTION Expressed predominantly in G2 phase.PTM Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.SIMILARITY Belongs to the MPI phosphatase family.
descriptionrecommendedName: M-phase inducer phosphatase 3 ecNumber evidence="21"3.1.3.48 alternativeName: Dual specificity phosphatase Cdc25C
geneNameCDC25C
identifierP30307
isSequenceChangedFALSE
keyword3D-structure
Acetylation
Alternative splicing
Cell cycle
Cell division
Host-virus interaction
Hydrolase
Mitosis
Nucleus
Phosphoprotein
Protein phosphatase
Proteomics identification
Reference proteome
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9909836] Weiser, Joel, 2024-05-14
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
[InstanceEdit:9963647] Weiser, Joel, 2025-08-15
nameCDC25C
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:6798274] ENSEMBL:ENSG00000158402 CDC25C [Homo sapiens]
secondaryIdentifierMPIP3_HUMAN
D3DQB8
Q96PL3
Q9H168
Q9H2E8
Q9H2E9
Q9H2F1
sequenceLength473
species[Species:48887] Homo sapiens
(isoformParent)[ReferenceIsoform:150493] UniProt:P30307-2 CDC25C [Homo sapiens]
[ReferenceIsoform:150494] UniProt:P30307-5 CDC25C [Homo sapiens]
[ReferenceIsoform:150495] UniProt:P30307-3 CDC25C [Homo sapiens]
[ReferenceIsoform:150496] UniProt:P30307-4 CDC25C [Homo sapiens]
[ReferenceIsoform:403599] UniProt:P30307-1 CDC25C [Homo sapiens]
(referenceEntity)[EntityWithAccessionedSequence:69741] CDC25C [nucleoplasm] [Homo sapiens]
[EntityWithAccessionedSequence:75003] p-S216-CDC25C [cytosol] [Homo sapiens]
[EntityWithAccessionedSequence:156496] p-S216-CDC25C [nucleoplasm] [Homo sapiens]
[EntityWithAccessionedSequence:156668] p-S198-CDC25C [nucleoplasm] [Homo sapiens]
[EntityWithAccessionedSequence:157879] CDC25C [cytosol] [Homo sapiens]
[EntityWithAccessionedSequence:6802968] p-S191-CDC25C [nucleoplasm] [Homo sapiens]
[EntityWithAccessionedSequence:8863654] p-T48,T67,S122,T130,S168,S214-CDC25C [cytosol] [Homo sapiens]
[EntityWithAccessionedSequence:8863664] p-T48,T67,S122,T130,S168,S214-CDC25C [nucleoplasm] [Homo sapiens]
[EntityWithAccessionedSequence:8981697] p-S198-CDC25C [cytosol] [Homo sapiens]
(referenceSequence)[ModifiedResidue:75806] O-phospho-L-serine at 216
[ModifiedResidue:156705] O-phospho-L-serine at 198
[ModifiedResidue:6802970] O-phospho-L-serine at 191
[ModifiedResidue:8863651] O-phospho-L-threonine at 67
[ModifiedResidue:8863652] O-phospho-L-serine at 168
[ModifiedResidue:8863655] O-phospho-L-serine at 122
[ModifiedResidue:8863656] O-phospho-L-threonine at 48
[ModifiedResidue:8863657] O-phospho-L-serine at 214
[ModifiedResidue:8863658] O-phospho-L-threonine at 130
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No pathways have been reviewed or authored by UniProt:P30307 CDC25C (95494)