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Details on Person yeast 2-hybrid evidence

Class:Id	EvidenceType:9030855
_displayName	yeast 2-hybrid evidence
_timestamp	2023-02-13 15:26:08
created	[InstanceEdit:9030887] Naithani, Sushma, 2017-11-29
definition	A type of bait-prey hybrid interaction evidence that is based on detection of protein-protein interaction by activation of a yeast reporter gene after a bait protein fused to a DNA-binding domain (which has been transfected into a yeast cell) is used to screen a cDNA library of clones fused to an activation domain. This ECO term has no GO counterpart - use its parent IPI for GO compliance
identifier	0000068
modified	[InstanceEdit:9826943] Wu, Guanming, 2023-01-30 [InstanceEdit:9828337] D'Eustachio, Peter, 2023-02-13
name	yeast 2-hybrid evidence
referenceDatabase	[ReferenceDatabase:9828336] ECO
(evidenceType)	[Reaction:9030825] Binding of SUB1A with SAB22 [Oryza sativa] [Reaction:9030869] Binding of SUB1C with SAB2 [Oryza sativa] [Reaction:9030883] Binding of SUB1C and SAB22 [Oryza sativa] [Reaction:9030947] Binding of SUB1A with SAB22 [Oryza sativa] [BlackBoxEvent:9916170] Interaction between OsEGT2 and OsOMT in nucleoplasm [Oryza sativa] [BlackBoxEvent:9916187] Interaction between OsEGT2 and OsOMT [Oryza sativa] [BlackBoxEvent:9916214] Interaction between OsEGT2 and OsHMT [Oryza sativa] [Polymerisation:9916245] Interaction between OsEGT2 and OsGXM [Oryza sativa]
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No pathways have been reviewed or authored by yeast 2-hybrid evidence (9030855)