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Details on Person UniProt:Q15004-2 PCLAF
| Class:Id | ReferenceIsoform:8970649 |
|---|---|
| _chainChangeLog | chain:1-111 added on Fri February 17 2017 |
| _displayName | UniProt:Q15004-2 PCLAF |
| _timestamp | 2024-11-03 19:47:45 |
| chain | chain:1-111 |
| checksum | FEF2C4E398B70E40 |
| comment | FUNCTION PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number.SUBUNIT Interacts (when monoubiquitinated at Lys-15 and Lys-24) with PCNA. Interacts with isoform 2/p33ING1b of ING1. Interacts with BRCA1.INTERACTION Following DNA damage, localizes to DNA damage sites (PubMed:21628590). Colocalizes with centrosomes in perinuclear region (PubMed:21673012).ALTERNATIVE PRODUCTS Expressed predominantly in liver, pancreas and placenta. Not detected in heart or brain. Highly expressed in a number of tumors, especially esophageal tumors, in anaplastic thyroid carcinomas, adrenocortical carcinomas, and in non-small-cell lung cancer lines.DEVELOPMENTAL STAGE Present only during S and G2 phases of the cell cycle. Peaks at the G2/M phase of the cell cycle and drops rapidly at mitotic exit in an APC/C-dependent manner (at protein level).INDUCTION By UV irradiation. By ATF3 in response to UV-stress.DOMAIN The PIP-box mediates the interaction with PCNA (PubMed:21628590, PubMed:23000965).DOMAIN The KEN box is required for the association with the APC/C complex.DOMAIN The D-box (destruction box) mediates the interaction with APC/C proteins, and acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.DOMAIN The initiation motif is required for efficient chain initiation by the APC/C complex E2 ligase UBE2C. It determines the rate of substrate's degradation without affecting its affinity for the APC/C, a mechanism used by the APC/C to control the timing of substrate proteolysis during the cell cycle (PubMed:21700221).PTM Monoubiquitinated at Lys-15 and Lys-24 during normal S phase, promoting its association with PCNA. Also diubiquitinated at these 2 sites. Following DNA damage, monoubiquitin chains at Lys-15 and Lys-24 are probably extended, leading to disrupt the interaction with PCNA. Polyubiquitinated by the APC/C complex at the mitotic exit, leading to its degradation by the proteasome.MISCELLANEOUS Overexpression in adrenocortical neoplasms (ACC), may promote growth and invasion in adrenal cancer.SEQUENCE CAUTION Extended N-terminus. |
| created | [InstanceEdit:8964659] Weiser, JD |
| description | recommendedName: fullName evidence="14"PCNA-associated factor alternativeName: Hepatitis C virus NS5A-transactivated protein 9 shortName: HCV NS5A-transactivated protein 9 alternativeName: Overexpressed in anaplastic thyroid carcinoma 1 shortName: OEATC-1 alternativeName: PCNA-associated factor of 15 kDa shortName: PAF15 shortName: p15PAF alternativeName: fullName evidence="16"PCNA-clamp-associated factor |
| geneName | PCLAF KIAA0101 NS5ATP9 PAF L5 |
| identifier | Q15004 |
| isoformParent | |
| isSequenceChanged | FALSE |
| keyword | 3D-structure Acetylation Alternative splicing Cytoplasm DNA damage DNA repair Isopeptide bond Nucleus Phosphoprotein Proteomics identification Reference proteome Ubl conjugation |
| modified | [InstanceEdit:9836292] Weiser, Joel, 2023-05-25 [InstanceEdit:9852000] Weiser, Joel, 2023-11-03 [InstanceEdit:9926675] Weiser, Joel, 2024-11-03 |
| name | PCLAF |
| referenceDatabase | [ReferenceDatabase:2] UniProt |
| referenceGene | [ReferenceDNASequence:8995025] ENSEMBL:ENSG00000166803 PCLAF [Homo sapiens] |
| secondaryIdentifier | PAF15_HUMAN A6NNU5 A8K3Y3 G9G694 G9G696 |
| sequenceLength | 111 |
| species | [Species:48887] Homo sapiens |
| variantIdentifier | Q15004-2 |
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No pathways have been reviewed or authored by UniProt:Q15004-2 PCLAF (8970649)
