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Details on Person UniProt:P12955-2 PEPD

Class:IdReferenceIsoform:8969799
_chainChangeLoginitiator methionine:1 added on Fri February 17 2017;chain:2-493 added on Fri February 17 2017;initiator methionine:1 for 8969799 removed on Fri Nov 03 2023;initiator methionine: for 8969799 added on Fri Nov 03 2023;initiator methionine: for 8969799 removed on Fri Aug 15 2025;initiator methionine:1 for 8969799 added on Fri Aug 15 2025
_displayNameUniProt:P12955-2 PEPD
_timestamp2025-08-15 21:47:41
chaininitiator methionine:1
chain:2-493
checksumE8C4A2497E44BA22
commentFUNCTION Dipeptidase that catalyzes the hydrolysis of dipeptides with a prolyl (Xaa-Pro) or hydroxyprolyl residue in the C-terminal position (PubMed:17081196, PubMed:35165443). The preferred dipeptide substrate is Gly-Pro, but other Xaa-Pro dipeptides, such as Ala-Pro, Met-Pro, Phe-Pro, Val-Pro and Leu-Pro, can be cleaved (PubMed:17081196). Plays an important role in collagen metabolism because the high level of iminoacids in collagen (PubMed:2925654).CATALYTIC ACTIVITY Xaa-L-Pro dipeptide + H2O = an L-alpha-amino acid + L-prolineCOFACTOR Binds 2 manganese ions per subunit.ACTIVITY REGULATION Specifically inhibited by the pseudodipeptide CQ31 (PubMed:35165443). Inhibition by CQ31 indirectly activates the CARD8 inflammasome: dipeptide accumulation following PEPD inactivation weaky inhibit dipeptidyl peptidases DDP8 and DPP9, relieving DPP8- and/or DPP9-mediated inhibition of CARD8 (PubMed:35165443).SUBUNIT Homodimer.INTERACTION The disease is caused by variants affecting the gene represented in this entry.SIMILARITY Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily.
created[InstanceEdit:8964659] Weiser, JD
descriptionrecommendedName: Xaa-Pro dipeptidase shortName: X-Pro dipeptidase ecNumber evidence="5 9"3.4.13.9 alternativeName: Imidodipeptidase alternativeName: Peptidase D alternativeName: fullName evidence="14"Proline dipeptidase shortName evidence="14"Prolidase
geneNamePEPD
PRD
identifierP12955
isoformParent
isSequenceChangedFALSE
keyword3D-structure
Acetylation
Alternative splicing
Collagen degradation
Dipeptidase
Direct protein sequencing
Disease variant
Hydrolase
Manganese
Metal-binding
Metalloprotease
Phosphoprotein
Protease
Proteomics identification
Reference proteome
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
[InstanceEdit:9963647] Weiser, Joel, 2025-08-15
namePEPD
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:8958615] ENSEMBL:ENSG00000124299 PEPD [Homo sapiens]
secondaryIdentifierPEPD_HUMAN
A8K3Z1
A8K416
A8K696
A8MX47
B4DDB7
B4DGJ1
E9PCE8
Q8TBN9
Q9BT75
sequenceLength493
species[Species:48887] Homo sapiens
variantIdentifierP12955-2
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No pathways have been reviewed or authored by UniProt:P12955-2 PEPD (8969799)