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Details on Person UniProt:P61077-3 UBE2D3

Class:IdReferenceIsoform:8967694
_chainChangeLogchain:1-147 added on Fri February 17 2017
_displayNameUniProt:P61077-3 UBE2D3
_timestamp2025-05-21 21:09:29
chainchain:1-147
checksumADD74A8A708EFEE3
commentFUNCTION Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:15247280, PubMed:15496420, PubMed:18284575, PubMed:20061386, PubMed:21532592, PubMed:28322253). In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination (PubMed:15247280, PubMed:15496420, PubMed:18284575, PubMed:20061386, PubMed:21532592). Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation (PubMed:20347421). Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin (PubMed:10329681). Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin (PubMed:10329681). Also acts as an initiator E2, in conjunction with RNF8, for the priming of PCNA (PubMed:18948756). Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase (PubMed:18948756). Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair (PubMed:16628214). Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus (PubMed:18515077). In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53 (PubMed:12646252, PubMed:15280377). In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes (PubMed:18508924). In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction (PubMed:11743028). Together with RNF135, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production (PubMed:28469175). Together with ZNF598, catalyzes ubiquitination of 40S ribosomal proteins in response to ribosome collisions (PubMed:28685749). In cooperation with the GATOR2 complex, catalyzes 'Lys-6'-linked ubiquitination of NPRL2 (PubMed:36528027).CATALYTIC ACTIVITY S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.CATALYTIC ACTIVITY S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.PATHWAY Protein modification; protein ubiquitination.SUBUNIT Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex; when Cullin is neddylated, the interaction between the E2 and the SCF complex is strengthened. Interacts with DAPK3. Interacts with BRCA1; the DNA damage checkpoint promotes the association with BRCA1 after ionizing radiation. Interacts non-covalently with ubiquitin. Interacts with E3 ubiquitin-protein ligase CBLC. Interacts with UBTD1 (PubMed:24211586). Interacts with RIGI and RNF135; involved in RIGI ubiquitination and activation (PubMed:28469175).INTERACTION Phosphorylated by AURKB.SIMILARITY Belongs to the ubiquitin-conjugating enzyme family.
created[InstanceEdit:8964659] Weiser, JD
descriptionrecommendedName: Ubiquitin-conjugating enzyme E2 D3 ecNumber evidence="18 25"2.3.2.23 alternativeName: (E3-independent) E2 ubiquitin-conjugating enzyme D3 ecNumber evidence="12"2.3.2.24 alternativeName: E2 ubiquitin-conjugating enzyme D3 alternativeName: Ubiquitin carrier protein D3 alternativeName: Ubiquitin-conjugating enzyme E2(17)KB 3 alternativeName: Ubiquitin-conjugating enzyme E2-17 kDa 3 alternativeName: Ubiquitin-protein ligase D3
geneNameUBE2D3
UBC5C
UBCH5C
identifierP61077
isoformParent
isSequenceChangedFALSE
keyword3D-structure
Alternative splicing
Apoptosis
ATP-binding
Cell membrane
Disulfide bond
DNA damage
DNA repair
Endosome
Membrane
Nucleotide-binding
Phosphoprotein
Proteomics identification
Reference proteome
Transferase
Ubl conjugation pathway
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9841277] Weiser, Joel
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9862192] Weiser, Joel, 2024-02-26
[InstanceEdit:9909836] Weiser, Joel, 2024-05-14
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9948485] Weiser, Joel, 2025-05-21
nameUBE2D3
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:9003903] ENSEMBL:ENSG00000109332 UBE2D3 [Homo sapiens]
secondaryIdentifierUB2D3_HUMAN
A6NJ93
A6NJB1
A6NM99
P47986
Q6IB88
Q6NXS4
Q8N924
sequenceLength147
species[Species:48887] Homo sapiens
variantIdentifierP61077-3
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