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Details on Person UniProt:A1A4I4 PKN1

Class:IdReferenceGeneProduct:797603
_chainChangeLoginitiator methionine:1 added on Fri February 6 2015;chain:2-944 added on Fri February 6 2015;initiator methionine:1 for 797603 removed on Fri Nov 03 2023;initiator methionine: for 797603 added on Fri Nov 03 2023;initiator methionine: for 797603 removed on Fri Aug 15 2025;initiator methionine:1 for 797603 added on Fri Aug 15 2025
_displayNameUniProt:A1A4I4 PKN1
_timestamp2025-08-15 21:40:24
chaininitiator methionine:1
chain:2-944
checksum11B6833ED4F8D57E
commentFUNCTION PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.CATALYTIC ACTIVITY L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)CATALYTIC ACTIVITY L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)ACTIVITY REGULATION Kinase activity is activated upon binding to Rho proteins (RHOA, RHOB and RAC1). Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by caspase-3 (CASP3) cleavage during apoptosis. Two specific sites, Thr-776 (activation loop of the kinase domain) and Ser-918 (turn motif), need to be phosphorylated for its full activation (By similarity).SUBUNIT Interacts with ZFAND6 (By similarity). Interacts with ANDR. Interacts with PRKCB. Interacts (via REM 1 and REM 2 repeats) with RAC1. Interacts (via REM 1 repeat) with RHOA. Interacts with RHOB. Interacts (via C-terminus) with PDPK1. Interacts with CCNT2; enhances MYOD1-dependent transcription. Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK (By similarity).SUBCELLULAR LOCATION Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-377 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis.DOMAIN The C1 domain does not bind the diacylglycerol (DAG).PTM Autophosphorylated; preferably on serine. Phosphorylated during mitosis.PTM Activated by limited proteolysis with trypsin.SIMILARITY Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
created[InstanceEdit:797602] Kanapin, AA
descriptionrecommendedName: Serine/threonine-protein kinase N1 ecNumber evidence="3"2.7.11.13 alternativeName: Protein kinase C-like 1 alternativeName: Protein kinase C-like PKN alternativeName: Protein kinase PKN-alpha alternativeName: Protein-kinase C-related kinase 1 alternativeName: Serine-threonine protein kinase N
geneNamePKN1
PKN
PRK1
PRKCL1
identifierA1A4I4
isSequenceChangedFALSE
keywordAcetylation
ATP-binding
Cell membrane
Chromatin regulator
Coiled coil
Cytoplasm
Endosome
Kinase
Membrane
Nucleotide-binding
Nucleus
Phosphoprotein
Reference proteome
Repeat
Serine/threonine-protein kinase
Transcription
Transcription regulation
Transferase
modified[InstanceEdit:937368] Yung, CK
[InstanceEdit:1042053] Yung, CK
[InstanceEdit:1220657] Yung, CK
[InstanceEdit:1300696] Yung, CK
[InstanceEdit:1301627] Yung, CK
[InstanceEdit:1551960] Weiser, JD
[InstanceEdit:1995863] Weiser, JD
[InstanceEdit:2132304] Weiser, JD
[InstanceEdit:2265580] Weiser, JD
[InstanceEdit:5433710] Weiser, JD
[InstanceEdit:5618415] Weiser, JD
[InstanceEdit:5634237] Weiser, JD
[InstanceEdit:5673015] Weiser, JD
[InstanceEdit:8869714] Weiser, JD
[InstanceEdit:8934940] Weiser, JD
[InstanceEdit:9037114] Weiser, JD
[InstanceEdit:9616384] Weiser, JD
[InstanceEdit:9627708] Weiser, JD
[InstanceEdit:9637257] Weiser, JD
[InstanceEdit:9657908] Weiser, JD
[InstanceEdit:9676415] Weiser, JD
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
[InstanceEdit:9963647] Weiser, Joel, 2025-08-15
namePKN1
referenceDatabase[ReferenceDatabase:2] UniProt
secondaryIdentifierPKN1_BOVIN
sequenceLength944
species[Species:48898] Bos taurus
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