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Details on Person In the first catalytic step of mRNA splicing, the 2' OH grou...

Class:IdSummation:72155
_displayNameIn the first catalytic step of mRNA splicing, the 2' OH grou...
_timestamp2003-09-03 03:54:22
created[InstanceEdit:72076] Krainer, AR, 2003-06-05 08:30:23
modified[InstanceEdit:75078] Joshi-Tope, G, 2003-08-22 11:27:00
[InstanceEdit:75826] Joshi-Tope, G, 2003-09-02 11:48:00
textIn the first catalytic step of mRNA splicing, the 2' OH group of the bulged A at the branch site performs a nucleophilic attack on the 5' splice site phosphodiester bond, resulting in cleavage of the bond between the 5' exon and the 5' end of the intron, and formation of a new bond between the 5' end of the intron and the branch site A. This results in a lariat-shaped intermediate, with the intron still attached to the 3' exon. The branch site A has a 2'-5' phosphodiester bond with the G at the beginning of the intron, in addition to the usual 5'-3' and 3'-5'phosphodiester bonds.
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