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Details on Person UniProt:Q92989 CLP1

Class:IdReferenceGeneProduct:72016
_chainChangeLogchain:1-425 added on Sat February 7 2015
_displayNameUniProt:Q92989 CLP1
_timestamp2024-11-03 20:13:46
chainchain:1-425
checksum640AF4768994CFE6
commentFUNCTION Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA (PubMed:24766809, PubMed:24766810). Its role in tRNA splicing and maturation is required for cerebellar development (PubMed:24766809, PubMed:24766810). Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.CATALYTIC ACTIVITY a 5'-end dephospho-2'-deoxyribonucleoside-DNA + ATP = a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + ADP + H(+)CATALYTIC ACTIVITY a 5'-end dephospho-ribonucleoside-RNA + ATP = a 5'-end 5'-phospho-ribonucleoside-RNA + ADP + H(+)COFACTOR Component of the tRNA splicing endonuclease complex, composed of CLP1, TSEN2, TSEN15, TSEN34 and TSEN54 (PubMed:24766809). Component of pre-mRNA cleavage complex II (CF-II). Also associates with numerous components of the pre-mRNA cleavage complex I (CF-I/CFIm), including NUDT21, CPSF2, CPSF3, CPSF6 and CPSF7. Interacts with CSTF2 and SYMPK.INTERACTION The disease is caused by variants affecting the gene represented in this entry. Neurodegeneration is due to defects in tRNA splicing (PubMed:24766809, PubMed:24766810).SIMILARITY Belongs to the Clp1 family. Clp1 subfamily.
descriptionrecommendedName: fullName evidence="1"Polyribonucleotide 5'-hydroxyl-kinase Clp1 ecNumber evidence="1"2.7.1.78 alternativeName: fullName evidence="1"Polyadenylation factor Clp1 alternativeName: fullName evidence="1"Polynucleotide kinase Clp1 alternativeName: fullName evidence="1"Pre-mRNA cleavage complex II protein Clp1
geneNameCLP1
HEAB
identifierQ92989
isSequenceChangedFALSE
keyword3D-structure
Alternative splicing
ATP-binding
Disease variant
Kinase
Magnesium
Manganese
mRNA processing
Neurodegeneration
Nickel
Nucleotide-binding
Nucleus
Proteomics identification
Reference proteome
Transferase
tRNA processing
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9917590] Weiser, Joel, 2024-08-09
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
nameCLP1
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:9000519] ENSEMBL:ENSG00000172409 CLP1 [Homo sapiens]
secondaryIdentifierCLP1_HUMAN
B2R7J6
B4DTI8
sequenceLength425
species[Species:48887] Homo sapiens
(isoformParent)[ReferenceIsoform:8972747] UniProt:Q92989-1 CLP1 [Homo sapiens]
[ReferenceIsoform:8972748] UniProt:Q92989-2 CLP1 [Homo sapiens]
(referenceEntity)[EntityWithAccessionedSequence:72017] CLP1 [nucleoplasm] [Homo sapiens]
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No pathways have been reviewed or authored by UniProt:Q92989 CLP1 (72016)