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Details on Person UniProt:O43809 NUDT21
| Class:Id | ReferenceGeneProduct:72009 |
|---|---|
| _chainChangeLog | initiator methionine:1 added on Sat February 7 2015;chain:2-227 added on Sat February 7 2015;initiator methionine:1 for 72009 removed on Fri Nov 03 2023;initiator methionine: for 72009 added on Fri Nov 03 2023;initiator methionine: for 72009 removed on Fri Aug 15 2025;initiator methionine:1 for 72009 added on Fri Aug 15 2025 |
| _displayName | UniProt:O43809 NUDT21 |
| _timestamp | 2025-08-15 21:59:45 |
| chain | initiator methionine:1 chain:2-227 |
| checksum | D204243E57F1CCC5 |
| comment | FUNCTION Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed:14690600, PubMed:15937220, PubMed:17024186, PubMed:17098938, PubMed:29276085, PubMed:8626397, PubMed:9659921). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed:14690600, PubMed:17024186, PubMed:8626397, PubMed:9659921). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed:17098938, PubMed:23187700, PubMed:29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed:17098938, PubMed:20695905, PubMed:29276085). NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7 (PubMed:29276085). Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing (PubMed:14690600, PubMed:15169763, PubMed:17024186, PubMed:20479262, PubMed:22813749, PubMed:8626397). The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA (PubMed:20479262, PubMed:21295486). Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function (By similarity). Binds to chromatin (By similarity). Binds to, but does not hydrolyze mono- and di-adenosine nucleotides (PubMed:18445629).SUBUNIT Homodimer (via N- and C-terminus); binds RNA as homodimer (PubMed:18445629, PubMed:20479262, PubMed:20695905). Component of the cleavage factor Im (CFIm) complex which is a heterotetramer composed of two subunits of NUDT21/CPSF5 and two subunits of CPSF6 or CPSF7 or a heterodimer of CPSF6 and CPSF7 (PubMed:14561889, PubMed:20695905, PubMed:21295486, PubMed:23187700, PubMed:8626397, PubMed:9659921). The cleavage factor Im (CFIm) complex associates with the CPSF and CSTF complexes to promote the assembly of the core mRNA 3'-processing machinery (PubMed:29276085). Interacts with CPSF6 (via the RRM domain); this interaction is direct and enhances binding to RNA (PubMed:14561889, PubMed:15169763, PubMed:17172643, PubMed:19864460, PubMed:29276085). Interacts with CPSF7 (PubMed:29276085, Ref.30). Interacts with FIP1L1; this interaction occurs in a RNA sequence-specific manner (PubMed:15937220). Interacts with PABPN1 (PubMed:15169763). Interacts (via N-terminus) with PAPOLA (via C-terminus); this interaction is direct and diminished by acetylation (PubMed:15169763, PubMed:17172643). Interacts with SNRNP70 (PubMed:14561889). Interacts with VIRMA (PubMed:29507755).INTERACTION Shuttles between the nucleus and the cytoplasm in a transcription- and XPO1/CRM1-independent manner, most probably in complex with the cleavage factor Im complex (CFIm) (PubMed:19864460). In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles (PubMed:15169763).TISSUE SPECIFICITY Expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.PTM Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 and SIRT2.SIMILARITY Belongs to the Nudix hydrolase family. CPSF5 subfamily.CAUTION Lacks the conserved metal-binding residues in the NUDIX motif and does not have hydrolase activity. |
| description | recommendedName: fullName evidence="23"Cleavage and polyadenylation specificity factor subunit 5 alternativeName: Cleavage and polyadenylation specificity factor 25 kDa subunit shortName: CPSF 25 kDa subunit alternativeName: fullName evidence="24"Cleavage factor Im complex 25 kDa subunit shortName evidence="24"CFIm25 alternativeName: Nucleoside diphosphate-linked moiety X motif 21 shortName: Nudix motif 21 alternativeName: fullName evidence="25"Nudix hydrolase 21 alternativeName: Pre-mRNA cleavage factor Im 68 kDa subunit |
| geneName | NUDT21 CFIM25 CPSF25 CPSF5 |
| identifier | O43809 |
| isSequenceChanged | FALSE |
| keyword | 3D-structure Acetylation Cytoplasm Differentiation Direct protein sequencing Methylation mRNA processing Nucleus Phosphoprotein Proteomics identification Reference proteome RNA-binding |
| modified | [InstanceEdit:9836292] Weiser, Joel, 2023-05-25 [InstanceEdit:9852000] Weiser, Joel, 2023-11-03 [InstanceEdit:9917590] Weiser, Joel, 2024-08-09 [InstanceEdit:9926675] Weiser, Joel, 2024-11-03 [InstanceEdit:9963647] Weiser, Joel, 2025-08-15 |
| name | NUDT21 |
| referenceDatabase | [ReferenceDatabase:2] UniProt |
| referenceGene | [ReferenceDNASequence:8988334] ENSEMBL:ENSG00000167005 NUDT21 [Homo sapiens] |
| secondaryIdentifier | CPSF5_HUMAN Q6IB85 Q6NE84 |
| sequenceLength | 227 |
| species | [Species:48887] Homo sapiens |
| (referenceEntity) | [EntityWithAccessionedSequence:72010] NUDT21 [nucleoplasm] [Homo sapiens] |
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No pathways have been reviewed or authored by UniProt:O43809 NUDT21 (72009)
