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Details on Person UniProt:Q9BUQ8 DDX23

Class:IdReferenceGeneProduct:71878
_chainChangeLogchain:1-820 added on Sat February 7 2015
_displayNameUniProt:Q9BUQ8 DDX23
_timestamp2025-02-21 19:58:09
chainchain:1-820
checksum01DD5BCF8BFBA2DB
commentFUNCTION Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation (PubMed:18425142). Independently of its spliceosome formation function, required for the suppression of incorrect R-loops formed during transcription; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA (PubMed:28076779).CATALYTIC ACTIVITY ATP + H2O = ADP + phosphate + H(+)SUBUNIT The phosphorylated form (by SRPK2) is a component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, WDR57, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39 (PubMed:16723661, PubMed:9409622). Identified in the spliceosome C complex (PubMed:11991638). Interacts with ERBB4 (PubMed:20858735). Interacts with ERCC6 (PubMed:26030138).INTERACTION During transcription, accumulates at chromatin loci where unscheduled R-loops form and colocalizes with paused 'Ser-5'-phosphorylated POLR2A/RNA polymerase II and kinase SRPK2.ALTERNATIVE PRODUCTS In vitro phosphorylated by CLK1 and U1 snRNP-associated protein kinase (PubMed:9409622). Phosphorylated by SRPK2 and this phosphorylation is required for its association with the tri-snRNP (U4/U6-U5 tri-small nuclear ribonucleoproteins) and subsequent spliceosomal B complex formation (PubMed:18425142). May be phosphorylated by SRPK2 on Ser residues in the SR domain; the phosphorylation is required for the removal of inappropriate R-loops during transcription (PubMed:28076779).SIMILARITY Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.
descriptionrecommendedName: fullName evidence="13"Probable ATP-dependent RNA helicase DDX23 ecNumber evidence="13"3.6.4.13 alternativeName: 100 kDa U5 snRNP-specific protein alternativeName: DEAD box protein 23 alternativeName: PRP28 homolog alternativeName: U5-100kD
geneNameDDX23
identifierQ9BUQ8
isSequenceChangedFALSE
keyword3D-structure
Alternative splicing
ATP-binding
Chromosome
Direct protein sequencing
Helicase
Hydrolase
Isopeptide bond
mRNA processing
mRNA splicing
Nucleotide-binding
Nucleus
Phosphoprotein
Proteomics identification
Reference proteome
Spliceosome
Ubl conjugation
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
nameDDX23
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:9004072] ENSEMBL:ENSG00000174243 DDX23 [Homo sapiens]
secondaryIdentifierDDX23_HUMAN
B2R600
B4DH15
O43188
sequenceLength820
species[Species:48887] Homo sapiens
(isoformParent)[ReferenceIsoform:8969611] UniProt:Q9BUQ8-1 DDX23 [Homo sapiens]
[ReferenceIsoform:8969612] UniProt:Q9BUQ8-2 DDX23 [Homo sapiens]
(referenceEntity)[EntityWithAccessionedSequence:71879] DDX23 [nucleoplasm] [Homo sapiens]
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No pathways have been reviewed or authored by UniProt:Q9BUQ8 DDX23 (71878)