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Details on Person UniProt:Q02224 CENPE

Class:IdReferenceGeneProduct:69726
_chainChangeLogchain:1-2698 added on Fri February 6 2015;propeptide:2699-2701 added on Fri February 6 2015
_displayNameUniProt:Q02224 CENPE
_timestamp2024-11-03 19:50:22
chainchain:1-2698
propeptide:2699-2701
checksum4BC59C2EF0B02D88
commentFUNCTION Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules (PubMed:23891108, PubMed:25395579, PubMed:7889940). The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated (PubMed:25908662). Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends (PubMed:23955301). Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized (PubMed:25743205). Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules (PubMed:17535814) The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss (By similarity).SUBUNIT Monomer (PubMed:15236970). Interacts with CENPF (PubMed:9763420). Interacts with BUB1B (PubMed:19625775, PubMed:9763420). Interacts with SEPT7 (PubMed:18460473). Interacts with KIF18A (PubMed:19625775). Interacts with PRC1 (PubMed:15297875). Interacts with NUF2; this interaction determines kinetochore localization (PubMed:17535814). Interacts with SKAP; this interaction greatly favors SKAP binding to microtubules (PubMed:22110139). Interacts with TRAPPC12 (PubMed:25918224). Interacts with CTCF (PubMed:25395579).INTERACTION Associates with kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division (By similarity). Recruited to the kinetochore in a SEPT7, CENPQ and TRAPPC12-dependent manner (PubMed:18460473, PubMed:25395579, PubMed:25918224). Recruited to the pericentromeric/centromeric regions of the chromosome in a CTCF-dependent manner (PubMed:26321640).ALTERNATIVE PRODUCTS The protein is composed of a N-terminal kinesin-motor domain involved in the chromosome movements, a long coil-coiled region involved in the homodimerization and an inhibitory C-tail involved in autoinhibition of the N-terminal catalytic part.PTM The C-terminal inhibitory domain is phosphorylated. Phosphorylation relieves autoinhibition of the kinetochore motor (By similarity).PTM Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association to the kinetochore.DISEASE The disease is caused by variants affecting the gene represented in this entry.SIMILARITY Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.SEQUENCE CAUTION Extended N-terminus.SEQUENCE CAUTION Aberrant splicing.
descriptionrecommendedName: Centromere-associated protein E alternativeName: Centromere protein E shortName: CENP-E alternativeName: fullName evidence="25"Kinesin-7 alternativeName: Kinesin-related protein CENPE
geneNameCENPE
identifierQ02224
isSequenceChangedFALSE
keyword3D-structure
Alternative splicing
ATP-binding
Cell cycle
Cell division
Centromere
Chromosome
Coiled coil
Cytoplasm
Cytoskeleton
Developmental protein
Direct protein sequencing
Disease variant
Kinetochore
Lipoprotein
Methylation
Microtubule
Mitosis
Motor protein
Nucleotide-binding
Phosphoprotein
Prenylation
Primary microcephaly
Proteomics identification
Reference proteome
Ubl conjugation
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9917590] Weiser, Joel, 2024-08-09
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
nameCENPE
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:8990015] ENSEMBL:ENSG00000138778 CENPE [Homo sapiens]
secondaryIdentifierCENPE_HUMAN
A6NKY9
A8K2U7
Q4LE75
sequenceLength2701
species[Species:48887] Homo sapiens
(isoformParent)[ReferenceIsoform:222825] UniProt:Q02224-3 CENPE [Homo sapiens]
[ReferenceIsoform:405061] UniProt:Q02224-1 CENPE [Homo sapiens]
(referenceEntity)[EntityWithAccessionedSequence:376245] CENPE [cytosol] [Homo sapiens]
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No pathways have been reviewed or authored by UniProt:Q02224 CENPE (69726)