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Details on Person Once the Mcm2-7 complex has been assembled onto the origin o...
| Class:Id | Summation:69342 |
|---|---|
| _displayName | Once the Mcm2-7 complex has been assembled onto the origin o... |
| _timestamp | 2011-12-22 15:21:04 |
| literatureReference | [LiteratureReference:69343] Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin [LiteratureReference:69345] Differential assembly of Cdc45p and DNA polymerases at early and late origins of DNA replication [LiteratureReference:69347] Assembly of a complex containing Cdc45p, replication protein A, and Mcm2p at replication origins controlled by S-phase cyclin-dependent kinases and Cdc7p-Dbf4p kinase. [LiteratureReference:69035] Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase [LiteratureReference:69348] DNA synthesis at individual replication forks requires the essential initiation factor Cdc45p [LiteratureReference:69349] Cell cycle-regulated nuclear import and export of Cdc47, a protein essential for initiation of DNA replication in budding yeast [LiteratureReference:69352] The Xenopus Xmus101 protein is required for the recruitment of Cdc45 to origins of DNA replication [LiteratureReference:69358] A DNA Damage-Regulated BRCT-Containing Protein, TopBP1, Is Required for Cell Survival [LiteratureReference:69361] Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae |
| modified | [InstanceEdit:141652] Gillespie, ME, 2004-09-25 00:17:00 [InstanceEdit:2026114] Orlic-Milacic, M, 2011-12-22 |
| text | Once the Mcm2-7 complex has been assembled onto the origin of replication, the next step is the assembly of Cdc45, an essential replication protein, in late G1. The assembly of Cdc45 onto origins of replication forms a complex distinct from the pre-replicative complex, sometimes called the pre-initiation complex. The assembly of Cdc45 onto origins correlates with the time of initiation. Like the Mcm2-7 proteins, Cdc45 binds specifically to origins in the G1 phase of the cell cycle and then to non-origin DNA during S phase and is therefore thought to travel with the replication fork. Indeed, S. cerevisiae Cdc45 is required for DNA replication elongation as well as replication initiation. Cdc45 is required for the association of alpha DNA polymerase:primase with chromatin. Based on this observation and the observation that in S. cerevisiae, cCdc45 has been found in large complexes with some components of Mcm2-7 complex, it has been suggested that Cdc45 plays a scaffolding role at the replication fork, coupling Pol-alpha:primase to the replication fork through the helicase. Association of Cdc45 with origin DNA is regulated in the cell cycle and its association is dependent on the activity of cyclin-dependent kinases but not the Cdc7/Dbf4 kinase. In Xenopus egg extracts, association of Cdc45 with chromatin is dependent on Xmus101. TopBP1, the human homolog of Xmus1, is essential for DNA replication and interacts with DNA polymerase epsilon, one of the polymerases involved in replicating the genome. TopBP1 homologs have been found in S. cerevisiae and S. pombe. Sld3, an additional protein required for Cdc45 association with chromatin in S. cerevisiae and S. pombe, has no known human homolog. |
| (summation) | [Reaction:68917] Cdc45 associates with the pre-replicative complex at the origin [Homo sapiens] |
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