Reactome: A Curated Pathway Database
THIS SITE IS USED FOR CURATION AND TESTING
IT IS NOT STABLE, IS LINKED TO AN INCOMPLETE DATA SET, AND IS NOT MONITORED FOR PERFORMANCE. WE STRONGLY RECOMMEND THE USE OF OUR PUBLIC SITE

Query author contributions in Reactome

Reactome depends on collaboration between our curation team and outside experts to assemble and peer-review its pathway modules. The integration of ORCID within Reactome enables us to meet a key challenge with authoring, curating and reviewing biological information by incentivizing and crediting the external experts that contribute their expertise and time to the Reactome curation process. More information is available at ORCID and Reactome.

If you have an ORCID ID that is not listed on this page, please forward this information to us and we will update your Reactome pathway records.

Name Email address

Details on Person UniProt:P35269 GTF2F1

Class:IdReferenceGeneProduct:65566
_chainChangeLoginitiator methionine:1 added on Fri February 6 2015;chain:2-517 added on Fri February 6 2015;initiator methionine:1 for 65566 removed on Fri Nov 03 2023;initiator methionine: for 65566 added on Fri Nov 03 2023;initiator methionine: for 65566 removed on Fri Aug 15 2025;initiator methionine:1 for 65566 added on Fri Aug 15 2025
_displayNameUniProt:P35269 GTF2F1
_timestamp2025-08-15 21:11:37
chaininitiator methionine:1
chain:2-517
checksum1032B04A36BDC24F
commentFUNCTION TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.SUBUNIT Heterodimer of an alpha and a beta subunit. Interacts with GTF2F2, CTDP1, TAF6/TAFII80 and URI1. Interacts with GTF2B (via C-terminus and preferentially via acetylated form); this interaction prevents binding of GTF2B to GTF2F2 (PubMed:12931194, PubMed:8662660). Part of TBP-based Pol II pre-initiation complex (PIC), in which Pol II core assembles with general transcription factors and other specific initiation factors including GTF2E1, GTF2E2, GTF2F1, GTF2F2, TCEA1, ERCC2, ERCC3, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2A1, GTF2A2, GTF2B and TBP; this large multi-subunit PIC complex mediates DNA unwinding and targets Pol II core to the transcription start site where the first phosphodiester bond forms.INTERACTION Up-regulated in response to enterovirus 71 (EV71) infection.PTM Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases.SIMILARITY Belongs to the TFIIF alpha subunit family.CAUTION Was reported to have a protein kinase activity and to autophosphorylates on Ser-385 and Thr-389.
descriptionrecommendedName: General transcription factor IIF subunit 1 alternativeName: General transcription factor IIF 74 kDa subunit alternativeName: Transcription initiation factor IIF subunit alpha shortName: TFIIF-alpha alternativeName: Transcription initiation factor RAP74
geneNameGTF2F1
RAP74
identifierP35269
isSequenceChangedFALSE
keyword3D-structure
Acetylation
Direct protein sequencing
DNA-binding
Nucleus
Phosphoprotein
Proteomics identification
Reference proteome
Transcription
Transcription regulation
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9909836] Weiser, Joel, 2024-05-14
[InstanceEdit:9917590] Weiser, Joel, 2024-08-09
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9963647] Weiser, Joel, 2025-08-15
nameGTF2F1
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:8998626] ENSEMBL:ENSG00000125651 GTF2F1 [Homo sapiens]
secondaryIdentifierT2FA_HUMAN
B2RCS0
Q9BWN0
sequenceLength517
species[Species:48887] Homo sapiens
(referenceEntity)[EntityWithAccessionedSequence:65565] GTF2F1 [nucleoplasm] [Homo sapiens]
[Change default viewing format]
No pathways have been reviewed or authored by UniProt:P35269 GTF2F1 (65566)