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Details on Person UniProt:P62487 POLR2G

Class:IdReferenceGeneProduct:63518
_chainChangeLogchain:1-172 added on Fri February 6 2015
_displayNameUniProt:P62487 POLR2G
_timestamp2024-11-03 19:58:21
chainchain:1-172
checksumAF3CE655F658CD5C
commentFUNCTION Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. POLR2G/RPB7 is part of a subcomplex with POLR2D/RPB4 that binds to a pocket formed by POLR2A/RPB1, POLR2B/RPB2 and POLR2F/RPABC2 at the base of the clamp element. The POLR2D/RPB4-POLR2G/RPB7 subcomplex seems to lock the clamp via POLR2G/RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The POLR2D/RPB4-POLR2G/RPB7 subcomplex binds single-stranded DNA and RNA.SUBUNIT Component of the RNA polymerase II (Pol II) core complex consisting of 12 subunits: a ten-subunit catalytic core composed of POLR2A/RPB1, POLR2B/RPB2, POLR2C/RPB3, POLR2I/RPB9, POLR2J/RPB11, POLR2E/RPABC1, POLR2F/RPABC2, POLR2H/RPABC3, POLR2K/RPABC4 and POLR2L/RPABC5 and a mobile stalk composed of two subunits POLR2D/RPB4 and POLR2G/RPB7, protruding from the core and functioning primarily in transcription initiation. Part of Pol II(G) complex, in which Pol II core associates with an additional subunit POLR2M; unlike conventional Pol II, Pol II(G) functions as a transcriptional repressor. Part of TBP-based Pol II pre-initiation complex (PIC), in which Pol II core assembles with general transcription factors and other specific initiation factors including GTF2E1, GTF2E2, GTF2F1, GTF2F2, TCEA1, ERCC2, ERCC3, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2A1, GTF2A2, GTF2B and TBP; this large multi-subunit PIC complex mediates DNA unwinding and targets Pol II core to the transcription start site where the first phosphodiester bond forms.INTERACTION Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.
descriptionrecommendedName: DNA-directed RNA polymerase II subunit RPB7 shortName: RNA polymerase II subunit B7 alternativeName: DNA-directed RNA polymerase II subunit G alternativeName: RNA polymerase II 19 kDa subunit shortName: RPB19
geneNamePOLR2G
RPB7
identifierP62487
isSequenceChangedFALSE
keyword3D-structure
DNA-directed RNA polymerase
Nucleus
Proteomics identification
Reference proteome
RNA-binding
Transcription
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9909836] Weiser, Joel, 2024-05-14
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
namePOLR2G
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:8993867] ENSEMBL:ENSG00000168002 POLR2G [Homo sapiens]
secondaryIdentifierRPB7_HUMAN
B2R5C0
P52433
Q2M1Z4
sequenceLength172
species[Species:48887] Homo sapiens
(referenceEntity)[EntityWithAccessionedSequence:63517] POLR2G [nucleoplasm] [Homo sapiens]
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