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Details on Person UniProt:P62745 RHOB

Class:IdReferenceGeneProduct:63082
_chainChangeLogchain:1-193 added on Fri February 6 2015;propeptide:194-196 added on Fri February 6 2015
_displayNameUniProt:P62745 RHOB
_timestamp2024-11-03 20:16:19
chainchain:1-193
propeptide:194-196
checksumCCE6FD53AE00CD83
commentFUNCTION Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.SUBUNIT Binds ROCK1 and ROCK2 (By similarity). Also binds PKN1/PRK1 (PubMed:9478917). Interacts with ARGGEF3 (PubMed:12221096). Interacts with RTKN (By similarity). Interacts with AKAP13 (PubMed:11546812). Interacts with RIPOR1 (PubMed:27807006).INTERACTION Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. Translocates to the equatorial region before furrow formation in a ECT2-dependent manner.INDUCTION Up-regulated by DNA damaging agents like H(2)O(2) or ionizing radiation (IR).PTM Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome.PTM (Microbial infection) Glycosylated at Tyr-34 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rho and leads to actin disassembly.PTM (Microbial infection) Glucosylated at Thr-37 by C.difficile toxins TcdA and TcdB in the colonic epithelium (PubMed:24905543). Monoglucosylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption (PubMed:24905543).MISCELLANEOUS RHOB is one of the targets of farnesyltransferase inhibitors which are currently under investigation as cancer therapeutics. These elevate the levels of geranylgeranylated RHOB and cause mislocalization, leading to apoptosis and antineoplastic effects.SIMILARITY Belongs to the small GTPase superfamily. Rho family.
descriptionrecommendedName: Rho-related GTP-binding protein RhoB alternativeName: Rho cDNA clone 6 shortName: h6
geneNameRHOB
ARH6
ARHB
identifierP62745
isSequenceChangedFALSE
keyword3D-structure
ADP-ribosylation
Angiogenesis
Apoptosis
Cell adhesion
Cell membrane
Developmental protein
Differentiation
Direct protein sequencing
Endosome
Glycoprotein
GTP-binding
Lipoprotein
Membrane
Methylation
Nucleotide-binding
Nucleus
Palmitate
Phosphoprotein
Prenylation
Protein transport
Proteomics identification
Reference proteome
Transport
Tumor suppressor
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
nameRHOB
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:9003194] ENSEMBL:ENSG00000143878 RHOB [Homo sapiens]
secondaryIdentifierRHOB_HUMAN
B2R692
P01121
Q5U0H6
Q7RTN5
Q7RTR9
Q9CUV7
sequenceLength196
species[Species:48887] Homo sapiens
(referenceEntity)[EntityWithAccessionedSequence:194855] RHOB [cytosol] [Homo sapiens]
[EntityWithAccessionedSequence:194891] RHOB [plasma membrane] [Homo sapiens]
[EntityWithAccessionedSequence:5666064] RHOB [endosome membrane] [Homo sapiens]
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