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Details on Person UniProt:Q01860 POU5F1

Class:IdReferenceGeneProduct:61960
_chainChangeLogchain:1-360 added on Sat February 7 2015
_displayNameUniProt:Q01860 POU5F1
_timestamp2026-02-20 23:09:37
chainchain:1-360
checksum934C58DAEA0C535B
commentFUNCTION Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.SUBUNIT Interacts with PKM (PubMed:18191611). Interacts with WWP2 (PubMed:19274063). Interacts with UBE2I and ZSCAN10 (By similarity). Interacts with PCGF1 (PubMed:26687479). Interacts with ESRRB; recruits ESRRB near the POU5F1-SOX2 element in the NANOG proximal promoter; the interaction is DNA independent (By similarity). Interacts with ZNF322 (By similarity). Interacts with MAPK8 and MAPK9; the interaction allows MAPK8 and MAPK9 to phosphorylate POU5F1 on Ser-355 (By similarity). Interacts (when phosphorylated on Ser-355) with FBXW8 (By similarity). Interacts with FBXW4 (By similarity). Interacts with SOX2 and SOX15; binds synergistically with either SOX2 or SOX15 to DNA (By similarity). Interacts with DDX56 (By similarity). Interacts with ZNF143; promoting POU5F1/OCT4 association with promoters (By similarity).INTERACTION Expressed in a diffuse and slightly punctuate pattern. Colocalizes with MAPK8 and MAPK9 in the nucleus.ALTERNATIVE PRODUCTS Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.DEVELOPMENTAL STAGE Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.INDUCTION Transcriptional activity is positively regulated by PKM (PubMed:18191611). Upon cell differentiation, expression is repressed by NR6A1 (PubMed:26769970).DOMAIN The POU-specific domain mediates interaction with PKM.DOMAIN The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.PTM Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.PTM Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.PTM ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.BIOTECHNOLOGY POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.MISCELLANEOUS Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.SIMILARITY Belongs to the POU transcription factor family. Class-5 subfamily.ONLINE INFORMATION Oct-4 entry
descriptionrecommendedName: POU domain, class 5, transcription factor 1 alternativeName: Octamer-binding protein 3 shortName: Oct-3 alternativeName: Octamer-binding protein 4 shortName: Oct-4 alternativeName: Octamer-binding transcription factor 3 shortName: OTF-3
geneNamePOU5F1
OCT3
OCT4
OTF3
identifierQ01860
isSequenceChangedFALSE
keyword3D-structure
Alternative splicing
Cytoplasm
Developmental protein
DNA-binding
Homeobox
Isopeptide bond
Nucleus
Phosphoprotein
Proteomics identification
Reference proteome
Transcription
Transcription regulation
Ubl conjugation
modified[InstanceEdit:84067] Schmidt, EE, 2003-12-18 04:29:09
[InstanceEdit:143527] Schmidt, EE, 2004-11-12 07:45:10
[InstanceEdit:217385] Schmidt, EE, 2008-03-27 06:23:53
[InstanceEdit:354386] Schmidt, EE, 2008-06-18 04:45:12
[InstanceEdit:384350] Kanapin, AA, 2008-11-26 14:00:39
[InstanceEdit:392885] Kanapin, AA, 2009-03-09 12:07:18
[InstanceEdit:400710] Schmidt, EE, 2009-03-25 05:33:35
[InstanceEdit:423310] Kanapin, AA
[InstanceEdit:435478] Kanapin, AA
[InstanceEdit:435871] Kanapin, AA
[InstanceEdit:447347] Kanapin, AA
[InstanceEdit:525883] Kanapin, AA
[InstanceEdit:613449] Kanapin, AA
[InstanceEdit:797602] Kanapin, AA
[InstanceEdit:937368] Yung, CK
[InstanceEdit:1042053] Yung, CK
[InstanceEdit:1220657] Yung, CK
[InstanceEdit:1300696] Yung, CK
[InstanceEdit:1301627] Yung, CK
[InstanceEdit:1551960] Weiser, JD
[InstanceEdit:1995863] Weiser, JD
[InstanceEdit:2132304] Weiser, JD
[InstanceEdit:2265580] Weiser, JD
[InstanceEdit:2587579] Weiser, JD
[InstanceEdit:4341137] Weiser, JD
[InstanceEdit:5433710] Weiser, JD
[InstanceEdit:5618415] Weiser, JD
[InstanceEdit:5634237] Weiser, JD
[InstanceEdit:5673015] Weiser, JD
[InstanceEdit:8987656] Weiser, JD
[InstanceEdit:9037114] Weiser, JD
[InstanceEdit:9607352] Weiser, JD
[InstanceEdit:9616384] Weiser, JD
[InstanceEdit:9637257] Weiser, JD
[InstanceEdit:9657908] Weiser, JD
[InstanceEdit:9676415] Weiser, JD
[InstanceEdit:9688885] Weiser, JD
[InstanceEdit:9698430] Weiser, JD
[InstanceEdit:9715482] Weiser, JD
[InstanceEdit:9773244] Weiser, Joel
[InstanceEdit:9829221] Weiser, Joel
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
[InstanceEdit:9963647] Weiser, Joel, 2025-08-15
[InstanceEdit:9983091] Weiser, Joel, 2026-02-20
namePOU5F1
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:2888888] ENSEMBL:ENSG00000204531 POU5F1 [Homo sapiens]
secondaryIdentifierPO5F1_HUMAN
A6NCS1
A6NLL8
D2IYK4
P31359
Q15167
Q15168
Q16422
Q5STF3
Q5STF4
sequenceLength360
species[Species:48887] Homo sapiens
(isoformParent)[ReferenceIsoform:152302] UniProt:Q01860-2 POU5F1 [Homo sapiens]
[ReferenceIsoform:405047] UniProt:Q01860-1 POU5F1 [Homo sapiens]
(referenceEntity)[EntityWithAccessionedSequence:452472] POU5F1 [nucleoplasm] [Homo sapiens]
[EntityWithAccessionedSequence:3246109] SUMO1-K123-POU5F1 [nucleoplasm] [Homo sapiens]
(referenceSequence)[GroupModifiedResidue:3246038] sumoylated lysine (monoSUMO1 [nucleoplasm]) at 123
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