Reactome: A Curated Pathway Database
THIS SITE IS USED FOR CURATION AND TESTING
IT IS NOT STABLE, IS LINKED TO AN INCOMPLETE DATA SET, AND IS NOT MONITORED FOR PERFORMANCE. WE STRONGLY RECOMMEND THE USE OF OUR PUBLIC SITE

Query author contributions in Reactome

Reactome depends on collaboration between our curation team and outside experts to assemble and peer-review its pathway modules. The integration of ORCID within Reactome enables us to meet a key challenge with authoring, curating and reviewing biological information by incentivizing and crediting the external experts that contribute their expertise and time to the Reactome curation process. More information is available at ORCID and Reactome.

If you have an ORCID ID that is not listed on this page, please forward this information to us and we will update your Reactome pathway records.

Name Email address

Details on Person UniProt:P00439 PAH

Class:IdReferenceGeneProduct:61638
_chainChangeLoginitiator methionine:1 added on Fri February 6 2015;chain:2-452 added on Fri February 6 2015
_displayNameUniProt:P00439 PAH
_timestamp2025-02-21 20:02:30
chainchain:1-452
checksum018F00EBBBDDCE2F
commentFUNCTION Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.CATALYTIC ACTIVITY (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + L-phenylalanine + O2 = (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin + L-tyrosineCOFACTOR N-terminal region of PAH is thought to contain allosteric binding sites for phenylalanine and to constitute an 'inhibitory' domain that regulates the activity of a catalytic domain in the C-terminal portion of the molecule.BIOPHYSICOCHEMICAL PROPERTIES Optimum temperature is 50 degrees Celsius.PATHWAY Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 1/6.SUBUNIT Homodimer and homotetramer.PTM Phosphorylation at Ser-16 increases basal activity and facilitates activation by the substrate phenylalanine.POLYMORPHISM The Glu-274 variant occurs on approximately 4% of African-American PAH alleles. The enzyme activity of the variant protein is indistinguishable from that of the wild-type form.DISEASE The disease is caused by variants affecting the gene represented in this entry.SIMILARITY Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.ONLINE INFORMATION Phenylalanine Hydroxylase Gene Locus-Specific DatabaseONLINE INFORMATION Phenylalanine hydroxylase entry
descriptionrecommendedName: Phenylalanine-4-hydroxylase shortName: PAH ecNumber evidence="22 24"1.14.16.1 alternativeName: Phe-4-monooxygenase
geneNamePAH
identifierP00439
isSequenceChangedFALSE
keyword3D-structure
Allosteric enzyme
Direct protein sequencing
Disease variant
Iron
Metal-binding
Monooxygenase
Oxidoreductase
Phenylalanine catabolism
Phenylketonuria
Phosphoprotein
Proteomics identification
Reference proteome
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
namePAH
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:8989137] ENSEMBL:ENSG00000171759 PAH [Homo sapiens]
secondaryIdentifierPH4H_HUMAN
Q16717
Q8TC14
sequenceLength452
species[Species:48887] Homo sapiens
(referenceEntity)[EntityWithAccessionedSequence:71066] PAH [cytosol] [Homo sapiens]
[EntityWithAccessionedSequence:2160450] S40L-PAH [cytosol] [Homo sapiens]
[EntityWithAccessionedSequence:5649486] PAH S40L [cytosol] [Homo sapiens]
(referenceSequence)[ReplacedResidue:2160460] L-serine 40 replaced with L-leucine
[Change default viewing format]
No pathways have been reviewed or authored by UniProt:P00439 PAH (61638)