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Details on Person UniProt:P17858 PFKL
| Class:Id | ReferenceGeneProduct:57809 |
|---|---|
| _chainChangeLog | initiator methionine:1 added on Fri February 6 2015;chain:2-780 added on Fri February 6 2015;initiator methionine:1 for 57809 removed on Fri Nov 03 2023;initiator methionine: for 57809 added on Fri Nov 03 2023;initiator methionine: for 57809 removed on Fri Aug 15 2025;initiator methionine:1 for 57809 added on Fri Aug 15 2025 |
| _displayName | UniProt:P17858 PFKL |
| _timestamp | 2025-08-15 21:17:44 |
| chain | initiator methionine:1 chain:2-780 |
| checksum | 0D686CE074E9626D |
| comment | FUNCTION Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (PubMed:22923583). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity).CATALYTIC ACTIVITY beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)COFACTOR Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate. GlcNAcylation by OGT overcomes allosteric regulation.PATHWAY Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.SUBUNIT Homo- and heterotetramers (By similarity). Phosphofructokinase (PFK) enzyme functions as a tetramer composed of different combinations of 3 types of subunits, called PFKM (where M stands for Muscle), PFKL (Liver) and PFKP (Platelet). The composition of the PFK tetramer differs according to the tissue type it is present in. In muscles, it is composed of 4 PFKM subunits (also called M4). In the liver, the predominant form is a tetramer of PFKL subunits (L4). In erythrocytes, both PFKM and PFKL subunits randomly tetramerize to form M4, L4 and other combinations (ML3, M2L2, M3L). The kinetic and regulatory properties of the tetrameric enzyme are dependent on the subunit composition, hence can vary across tissues (Probable).INTERACTION GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway. Glycosylation is stimulated by both hypoxia and glucose deprivation.MISCELLANEOUS In human PFK exists as a system of 3 types of subunits, PFKM (muscle), PFKL (liver) and PFKP (platelet) isoenzymes.MISCELLANEOUS Glycosylation may play a role in cancer cell proliferation: inhibition of 6-phosphofructokinase activity and subsequent redirection of the glucose flux through the oxidative pentose phosphate pathway confers a selective growth advantage on cancer cells. Moreover GlcNAcylation is observed in multiple cancer cell lines and tissue samples and GlcNAcylation leads to larger xenografts tunors in mice (PubMed:22923583).SIMILARITY Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily. |
| description | recommendedName: fullName evidence="3"ATP-dependent 6-phosphofructokinase, liver type shortName evidence="3"ATP-PFK shortName: PFK-L ecNumber evidence="3"2.7.1.11 alternativeName: 6-phosphofructokinase type B alternativeName: Phosphofructo-1-kinase isozyme B shortName: PFK-B alternativeName: fullName evidence="3"Phosphohexokinase |
| geneName | PFKL |
| identifier | P17858 |
| isSequenceChanged | FALSE |
| keyword | 3D-structure Acetylation Allosteric enzyme Alternative splicing ATP-binding Cytoplasm Direct protein sequencing Glycolysis Glycoprotein Kinase Magnesium Metal-binding Nucleotide-binding Phosphoprotein Proteomics identification Reference proteome Transferase |
| modified | [InstanceEdit:9836292] Weiser, Joel, 2023-05-25 [InstanceEdit:9852000] Weiser, Joel, 2023-11-03 [InstanceEdit:9926675] Weiser, Joel, 2024-11-03 [InstanceEdit:9939033] Weiser, Joel, 2025-02-21 [InstanceEdit:9963647] Weiser, Joel, 2025-08-15 |
| name | PFKL |
| referenceDatabase | [ReferenceDatabase:2] UniProt |
| referenceGene | [ReferenceDNASequence:8990906] ENSEMBL:ENSG00000141959 PFKL [Homo sapiens] |
| secondaryIdentifier | PFKAL_HUMAN Q96A64 Q96IH4 Q9BR91 |
| sequenceLength | 780 |
| species | [Species:48887] Homo sapiens |
| (isoformParent) | [ReferenceIsoform:149140] UniProt:P17858-2 PFKL [Homo sapiens] [ReferenceIsoform:403114] UniProt:P17858-1 PFKL [Homo sapiens] |
| (referenceEntity) | [EntityWithAccessionedSequence:70459] PFKL [cytosol] [Homo sapiens] [EntityWithAccessionedSequence:6800876] PFKL [secretory granule lumen] [Homo sapiens] [EntityWithAccessionedSequence:6801030] PFKL [ficolin-1-rich granule lumen] [Homo sapiens] [EntityWithAccessionedSequence:6801043] PFKL [extracellular exosome] [Homo sapiens] [EntityWithAccessionedSequence:6801044] PFKL [extracellular exosome] [Homo sapiens] [EntityWithAccessionedSequence:6806471] PFKL [extracellular region] [Homo sapiens] |
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No pathways have been reviewed or authored by UniProt:P17858 PFKL (57809)
