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Details on Person The human isoaspartyl peptidase/L-asparaginase (ASRGL1) is a...

Class:IdSummation:5696363
_displayNameThe human isoaspartyl peptidase/L-asparaginase (ASRGL1) is a...
_timestamp2025-02-22 21:59:40
created[InstanceEdit:5696360] Jassal, Bijay, 2015-05-28
modified[InstanceEdit:5696383] Jassal, Bijay, 2015-05-28
[InstanceEdit:6783363] Jassal, Bijay, 2015-06-12
[InstanceEdit:9939282] D'Eustachio, Peter, 2025-02-22
textThe human isoaspartyl peptidase/L-asparaginase (ASRGL1) is an N-terminal nucleophile (Ntn) hydrolase superfamily member that catalyses the hydrolysis of l-asparagine and aspartyl-dipeptides and their methyl esters such as the artificial sweetener aspartame (alpha-L-Asp-L-Phe methyl ester), annotated here (Cantor et al. 2009, Li et al. 2012, Nomme et al. 2014). ASRGL1, localized to the cytosol, is active as a heterodimer formed by autocleavage between Gly167 and Thr168 (Nomme et al. 2012).

The enzyme is expressed in brain, kidney, testis and the gastrointestinal tract. Its physiological substrates are not known, and its role in aspartame metabolism may be limited in vivo, as most dietary aspartame is broken down by other known peptidases associated with intestinal microvilli (Hooper et al. 1994).

(summation)[Reaction:5696365] ASRGL1 hydrolyses aspartame to L-Asp, L-Phe, MeOH [Homo sapiens]
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