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Details on Person UniProt:C7EXK4 ATP8A2

Class:IdReferenceGeneProduct:5618418
_chainChangeLogchain:1-1148 added on Sat February 7 2015
_displayNameUniProt:C7EXK4 ATP8A2
_timestamp2025-02-21 18:54:26
chainchain:1-1148
checksum9D0DC179F8BF62EF
commentFUNCTION Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids (PubMed:19778899, PubMed:24706822, PubMed:26592152, PubMed:31371510). Able to translocate phosphatidylserine, but not phosphatidylcholine (By similarity). Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. Reconstituted to liposomes, the ATP8A2:TMEM30A flippase complex predominantly transports phosphatidylserine (PS) and to a lesser extent phosphatidylethanolamine (PE) (PubMed:19778899, PubMed:24706822, PubMed:26592152, PubMed:31371510). Phospholipid translocation is not associated with a countertransport of an inorganic ion or other charged substrate from the cytoplasmic side toward the exoplasm in connection with the phosphorylation from ATP (PubMed:31371510). ATP8A2:TMEM30A may be involved in regulation of neurite outgrowth. Proposed to function in the generation and maintenance of phospholipid asymmetry in photoreceptor disk membranes and neuronal axon membranes. May be involved in vesicle trafficking in neuronal cells. Required for normal visual and auditory function; involved in photoreceptor and inner ear spiral ganglion cell survival.CATALYTIC ACTIVITY ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.CATALYTIC ACTIVITY a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)CATALYTIC ACTIVITY a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ADP + phosphate + H(+)COFACTOR ATPase activity is stimulated by phosphatidylserine (PS) and minimally by phosphatidylethanolamine (PE). ATPase activity is inhibited by N-ethylmaleimide (NEM) and vanadate. Flippase activity is inhibited by NEM and 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS).SUBUNIT Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit and an accessory beta subunit (PubMed:21454556, PubMed:26592152). Interacts with TMEM30A to form a flippase complex (PubMed:21454556).SUBCELLULAR LOCATION Localizes to the Golgi and endosomes in photoreceptor cells (By similarity). Localizes to disk membranes of rod photoreceptor outer segments (ROS) (PubMed:21454556).TISSUE SPECIFICITY Expressed in retinal photoreceptor cells and testis.SIMILARITY Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.SEQUENCE CAUTION Extended N-terminus.
created[InstanceEdit:5618415] Weiser, JD
descriptionrecommendedName: fullName evidence="4"Phospholipid-transporting ATPase IB ecNumber evidence="8 10 11 12 13"7.6.2.1 alternativeName: ATPase class I type 8A member 2 alternativeName: P4-ATPase flippase complex alpha subunit ATP8A2
geneNameATP8A2
identifierC7EXK4
isSequenceChangedFALSE
keywordATP-binding
Cell membrane
Cell projection
Endosome
Golgi apparatus
Lipid transport
Magnesium
Membrane
Metal-binding
Nucleotide-binding
Phosphoprotein
Reference proteome
Translocase
Transmembrane
Transmembrane helix
Transport
modified[InstanceEdit:5634237] Weiser, JD
[InstanceEdit:5673015] Weiser, JD
[InstanceEdit:5691543] Weiser, JD
[InstanceEdit:9037114] Weiser, JD
[InstanceEdit:9627708] Weiser, JD
[InstanceEdit:9637257] Weiser, JD
[InstanceEdit:9657908] Weiser, JD
[InstanceEdit:9676415] Weiser, JD
[InstanceEdit:9715482] Weiser, JD
[InstanceEdit:9730071] Weiser, JD
[InstanceEdit:9750299] Weiser, JD
[InstanceEdit:9829221] Weiser, Joel
[InstanceEdit:9841277] Weiser, Joel
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9917590] Weiser, Joel, 2024-08-09
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
nameATP8A2
referenceDatabase[ReferenceDatabase:2] UniProt
secondaryIdentifierAT8A2_BOVIN
sequenceLength1148
species[Species:48898] Bos taurus
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