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Details on Person UniProt:P06733 ENO1

Class:Id	ReferenceGeneProduct:54312
_chainChangeLog	initiator methionine:1 added on Fri February 6 2015;chain:2-434 added on Fri February 6 2015;initiator methionine:1 for 54312 removed on Fri Nov 03 2023;initiator methionine: for 54312 added on Fri Nov 03 2023;initiator methionine: for 54312 removed on Fri Aug 15 2025;initiator methionine:1 for 54312 added on Fri Aug 15 2025
_displayName	UniProt:P06733 ENO1
_timestamp	2026-02-20 22:32:02
chain	initiator methionine:1 chain:2-434
checksum	A0ED663FCC15ADA5
comment	FUNCTION Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis (PubMed:1369209, PubMed:29775581). Also involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:10802057, PubMed:12666133, PubMed:2005901, PubMed:29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133). Stimulates immunoglobulin production (PubMed:1369209).FUNCTION Binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.CATALYTIC ACTIVITY (2R)-2-phosphoglycerate = phosphoenolpyruvate + H2OCOFACTOR Binds two Mg(2+) per subunit. Required for catalysis and for stabilizing the dimer.BIOPHYSICOCHEMICAL PROPERTIES Enolase activity is lost above pH 9.0. Immunoglobulin production stimulating activity is retained at pH 13.0.PATHWAY Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.SUBUNIT Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific (PubMed:18560153). ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding (PubMed:9308760). Isoform MBP-1 interacts with TRAPPC2B (PubMed:11134351). Interacts with ENO4 and PGAM2 (By similarity). Interacts with CMTM6 (PubMed:28813417).INTERACTION Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.SUBCELLULAR LOCATION The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.DEVELOPMENTAL STAGE During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.INDUCTION Induced in diffuse large cell lymphoma (DLCL) after treatment with the natural biological agent, Bryo1. Up-regulated in response to enterovirus 71 (EV71) infection (at protein level).PTM ISGylated.PTM Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity.MISCELLANEOUS Used as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also marker for endometriosis. Antibodies against alpha-enolase are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. Is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism.MISCELLANEOUS It is uncertain whether the alternative initiation site is at Met-94 or at Met-97.SIMILARITY Belongs to the enolase family.SEQUENCE CAUTION Sequencing errors.
description	recommendedName: fullName evidence="23"Alpha-enolase ecNumber evidence="9 17"4.2.1.11 alternativeName: 2-phospho-D-glycerate hydro-lyase alternativeName: fullName evidence="22"C-myc promoter-binding protein alternativeName: Enolase 1 alternativeName: MBP-1 alternativeName: MPB-1 alternativeName: Non-neural enolase shortName: NNE alternativeName: Phosphopyruvate hydratase alternativeName: Plasminogen-binding protein
geneName	ENO1 ENO1L1 MBPB1 MPB1
identifier	P06733
isSequenceChanged	FALSE
keyword	3D-structure Acetylation Alternative initiation Cell membrane Cytoplasm Direct protein sequencing DNA-binding Glycolysis Hydroxylation Isopeptide bond Lyase Magnesium Membrane Metal-binding Nucleus Phosphoprotein Plasminogen activation Proteomics identification Reference proteome Repressor Transcription Transcription regulation Ubl conjugation
modified	[InstanceEdit:9836292] Weiser, Joel, 2023-05-25 [InstanceEdit:9841277] Weiser, Joel [InstanceEdit:9852000] Weiser, Joel, 2023-11-03 [InstanceEdit:9917590] Weiser, Joel, 2024-08-09 [InstanceEdit:9926675] Weiser, Joel, 2024-11-03 [InstanceEdit:9939033] Weiser, Joel, 2025-02-21 [InstanceEdit:9963647] Weiser, Joel, 2025-08-15 [InstanceEdit:9983091] Weiser, Joel, 2026-02-20
name	ENO1
referenceDatabase	[ReferenceDatabase:2] UniProt
referenceGene	[ReferenceDNASequence:8957960] ENSEMBL:ENSG00000074800 ENO1 [Homo sapiens]
secondaryIdentifier	ENOA_HUMAN B2RD59 P22712 Q16704 Q4TUS4 Q53FT9 Q53HR3 Q658M5 Q6GMP2 Q71V37 Q7Z3V6 Q8WU71 Q96GV1 Q9BT62 Q9UCH6 Q9UM55
sequenceLength	434
species	[Species:48887] Homo sapiens
(isoformParent)	[ReferenceIsoform:228302] UniProt:P06733-2 ENO1 [Homo sapiens] [ReferenceIsoform:402510] UniProt:P06733-1 ENO1 [Homo sapiens]
(referenceEntity)	[EntityWithAccessionedSequence:71437] ENO1 [cytosol] [Homo sapiens] [EntityWithAccessionedSequence:8957937] ENO1 [extracellular region] [Homo sapiens]
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No pathways have been reviewed or authored by UniProt:P06733 ENO1 (54312)