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Details on Person UniProt:O75907 DGAT1

Class:IdReferenceGeneProduct:53580
_chainChangeLogchain:1-488 added on Sat February 7 2015
_displayNameUniProt:O75907 DGAT1
_timestamp2026-02-20 21:29:04
chainchain:1-488
checksum6574D5DBF15D6171
commentFUNCTION Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates (PubMed:16214399, PubMed:18768481, PubMed:28420705, PubMed:32433610, PubMed:32433611, PubMed:9756920). Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats (PubMed:18768481). In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage (PubMed:16214399). Also present in female mammary glands, where it produces fat in the milk (By similarity). May be involved in VLDL (very low density lipoprotein) assembly (PubMed:18768481). In contrast to DGAT2 it is not essential for survival (By similarity). Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders (PubMed:16214399). Exhibits additional acyltransferase activities, includin acyl CoA:monoacylglycerol acyltransferase (MGAT), wax monoester and wax diester synthases (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed:28420705).CATALYTIC ACTIVITY an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoACATALYTIC ACTIVITY all-trans-retinol + an acyl-CoA = an all-trans-retinyl ester + CoACATALYTIC ACTIVITY 2-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoyl-CoA = 1,2-di-(9Z-octadecenoyl)-sn-glycerol + CoACATALYTIC ACTIVITY 1,2-di-(9Z-octadecenoyl)-sn-glycerol + (9Z)-octadecenoyl-CoA = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoACATALYTIC ACTIVITY all-trans-retinol + hexadecanoyl-CoA = all-trans-retinyl hexadecanoate + CoACATALYTIC ACTIVITY 1-O-(9Z-octadecenyl)-glycerol + (9Z)-octadecenoyl-CoA = 1-O-(9Z-octadecyl)-3-(9Z-octadecenoyl)-glycerol + CoACATALYTIC ACTIVITY 1-O-(9Z-octadecyl)-3-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoyl-CoA = 1-O-(9Z-octadecenyl)-2,3-di-(9Z-octadecenoyl)glycerol + CoACATALYTIC ACTIVITY 1-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoyl-CoA = 1,2-di-(9Z-octadecenoyl)-glycerol + CoACATALYTIC ACTIVITY 1,2-di-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+) = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2OCATALYTIC ACTIVITY 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + (9Z)-octadecenoyl-CoA = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-3-(9Z)-octadecenoyl-sn-glycerol + CoACATALYTIC ACTIVITY hexadecane-1,2-diol + 2 hexadecanoyl-CoA = 1,2-O,O-dihexadecanoyl-1,2-hexadecanediol + 2 CoACATALYTIC ACTIVITY hexadecane-1,2-diol + hexadecanoyl-CoA = 2-hydroxyhexadecyl hexadecanoate + CoACATALYTIC ACTIVITY 2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoACATALYTIC ACTIVITY 1,2-di-(9Z-octadecenoyl)-sn-glycerol + hexadecanoyl-CoA = 1,2-di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + CoACATALYTIC ACTIVITY hexadecan-1-ol + hexadecanoyl-CoA = hexadecyl hexadecanoate + CoACATALYTIC ACTIVITY 13-cis-retinol + hexadecanoyl-CoA = 13-cis-retinyl hexadecanoate + CoACATALYTIC ACTIVITY 1,3-di-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoyl-CoA = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoACATALYTIC ACTIVITY 2,3-di-(9Z)-octadecenoyl-sn-glycerol + (9Z)-octadecenoyl-CoA = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoAACTIVITY REGULATION XP620 is a selective DGAT1 inhibitor.BIOPHYSICOCHEMICAL PROPERTIES Lipid metabolism; glycerolipid metabolism.SUBUNIT Homodimer or homotetramer; both forms have similar enzymatic activities.INTERACTION The disordered N-terminal region is required for the diacylglycerol O-acyltransferase activity and may regulate enzymatic function via its interaction with the MBOAT fold.DOMAIN The MBOAT fold forms a reaction chamber in the endoplasmic reticulum membrane that encloses the active sites (PubMed:32433610, PubMed:32433611). The reaction chamber has a tunnel to the cytosolic side and its entrance recognizes the hydrophilic CoA motif of an acyl-CoA molecule (PubMed:32433610). The chamber has separate entrances for each of the two substrates, acyl-CoA and 1,2-diacyl-sn-glycerol (PubMed:32433610).DISEASE The disease is caused by variants affecting the gene represented in this entry.SIMILARITY Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.
descriptionrecommendedName: fullName evidence="15"Diacylglycerol O-acyltransferase 1 ecNumber evidence="4 9 10"2.3.1.20 alternativeName: ACAT-related gene product 1 alternativeName: fullName evidence="12"Acyl-CoA retinol O-fatty-acyltransferase shortName evidence="12"ARAT shortName evidence="12"Retinol O-fatty-acyltransferase ecNumber evidence="4"2.3.1.76 alternativeName: Diglyceride acyltransferase
geneNameDGAT1
AGRP1
DGAT
identifierO75907
isSequenceChangedFALSE
keyword3D-structure
Acyltransferase
Disease variant
Endoplasmic reticulum
Lipid metabolism
Membrane
Phosphoprotein
Proteomics identification
Reference proteome
Transferase
Transmembrane
Transmembrane helix
modified[InstanceEdit:84067] Schmidt, EE, 2003-12-18 04:29:09
[InstanceEdit:143527] Schmidt, EE, 2004-11-12 07:45:10
[InstanceEdit:217385] Schmidt, EE, 2008-03-27 06:23:53
[InstanceEdit:354386] Schmidt, EE, 2008-06-18 04:45:12
[InstanceEdit:384350] Kanapin, AA, 2008-11-26 14:00:39
[InstanceEdit:392885] Kanapin, AA, 2009-03-09 12:07:18
[InstanceEdit:400710] Schmidt, EE, 2009-03-25 05:33:35
[InstanceEdit:423310] Kanapin, AA
[InstanceEdit:435478] Kanapin, AA
[InstanceEdit:435871] Kanapin, AA
[InstanceEdit:447347] Kanapin, AA
[InstanceEdit:525883] Kanapin, AA
[InstanceEdit:613449] Kanapin, AA
[InstanceEdit:797602] Kanapin, AA
[InstanceEdit:937368] Yung, CK
[InstanceEdit:1042053] Yung, CK
[InstanceEdit:1220657] Yung, CK
[InstanceEdit:1300696] Yung, CK
[InstanceEdit:1301627] Yung, CK
[InstanceEdit:1551960] Weiser, JD
[InstanceEdit:1995863] Weiser, JD
[InstanceEdit:2132304] Weiser, JD
[InstanceEdit:2265580] Weiser, JD
[InstanceEdit:2587579] Weiser, JD
[InstanceEdit:5263031] Weiser, JD
[InstanceEdit:5433710] Weiser, JD
[InstanceEdit:5618415] Weiser, JD
[InstanceEdit:5634237] Weiser, JD
[InstanceEdit:5673015] Weiser, JD
[InstanceEdit:5691543] Weiser, JD
[InstanceEdit:8856987] Weiser, JD
[InstanceEdit:8987656] Weiser, JD
[InstanceEdit:9037114] Weiser, JD
[InstanceEdit:9627708] Weiser, JD
[InstanceEdit:9637257] Weiser, JD
[InstanceEdit:9666080] Weiser, JD
[InstanceEdit:9676415] Weiser, JD
[InstanceEdit:9698430] Weiser, JD
[InstanceEdit:9706439] Weiser, JD
[InstanceEdit:9715482] Weiser, JD
[InstanceEdit:9730071] Weiser, JD
[InstanceEdit:9767224] Weiser, Joel
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9862192] Weiser, Joel, 2024-02-26
[InstanceEdit:9917590] Weiser, Joel, 2024-08-09
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
[InstanceEdit:9948485] Weiser, Joel, 2025-05-21
[InstanceEdit:9983091] Weiser, Joel, 2026-02-20
nameDGAT1
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:8995939] ENSEMBL:ENSG00000185000 DGAT1 [Homo sapiens]
secondaryIdentifierDGAT1_HUMAN
B2RWQ2
D3DWL6
Q96BB8
sequenceLength488
species[Species:48887] Homo sapiens
(referenceEntity)[EntityWithAccessionedSequence:53579] DGAT1 [endoplasmic reticulum membrane] [Homo sapiens]
[EntityWithAccessionedSequence:6799564] DGAT1 [specific granule membrane] [Homo sapiens]
[EntityWithAccessionedSequence:6806313] DGAT1 [plasma membrane] [Homo sapiens]
[EntityWithAccessionedSequence:9977034] PolyUb-DGAT1 [endoplasmic reticulum membrane] [Homo sapiens]
(referenceSequence)[GroupModifiedResidue:9977031] ubiquitinylated lysine (polyubiquitin chain [cytosol]) at unknown position
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