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Details on Person UniProt:Q9NUV7 SPTLC3

Class:IdReferenceGeneProduct:52918
_chainChangeLogchain:1-552 added on Fri February 6 2015
_displayNameUniProt:Q9NUV7 SPTLC3
_timestamp2025-02-21 18:52:05
chainchain:1-552
checksumEED341220DCA683A
commentFUNCTION Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases (PubMed:19416851, PubMed:19648650). The SPT complex is composed of SPTLC1, SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer consisting of SPTLC1 and SPTLC2/SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference (PubMed:19416851). The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference (PubMed:19416851, PubMed:19648650).CATALYTIC ACTIVITY L-serine + hexadecanoyl-CoA + H(+) = 3-oxosphinganine + CO2 + CoACATALYTIC ACTIVITY dodecanoyl-CoA + L-serine + H(+) = 3-oxotetradecasphinganine + CO2 + CoACATALYTIC ACTIVITY tetradecanoyl-CoA + L-serine + H(+) = 3-oxohexadecasphinganine + CO2 + CoACATALYTIC ACTIVITY octadecanoyl-CoA + L-serine + H(+) = 3-oxoeicosasphinganine + CO2 + CoACOFACTOR SPT complex catalytic activity is negatively regulated by ORMDL proteins, including ORMDL3, in the presence of ceramides (By similarity). This mechanism allows to maintain ceramide levels at sufficient concentrations for the production of complex sphingolipids, but which prevents the accumulation of ceramides to levels that trigger apoptosis (Probable).BIOPHYSICOCHEMICAL PROPERTIES Lipid metabolism; sphingolipid metabolism.SUBUNIT Component of the serine palmitoyltransferase (SPT) complex, which is composed of SPTLC1, SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. The heterodimer consisting of SPTLC1 and SPTLC2/SPTLC3 forms the catalytic core of the enzyme, while SPTSSA or SPTSSB subunits determine substrate specificity (PubMed:19416851). SPT also interacts with ORMDL proteins, especially ORMDL3, which negatively regulate SPT activity in the presence of ceramides (By similarity).INTERACTION Expressed in most tissues, except peripheral blood cells and bone marrow, with highest levels in heart, kidney, liver, uterus and skin.SIMILARITY Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.SEQUENCE CAUTION Contaminating sequence. Potential poly-A sequence.
descriptionrecommendedName: fullName evidence="12"Serine palmitoyltransferase 3 ecNumber evidence="7 8"2.3.1.50 alternativeName: Long chain base biosynthesis protein 2b shortName evidence="11"LCB2b alternativeName: Long chain base biosynthesis protein 3 shortName: LCB 3 alternativeName: Serine-palmitoyl-CoA transferase 3 shortName: SPT 3
geneNameSPTLC3
C20orf38
SPTLC2L
identifierQ9NUV7
isSequenceChangedFALSE
keywordAcyltransferase
Alternative splicing
Endoplasmic reticulum
Lipid metabolism
Membrane
Proteomics identification
Pyridoxal phosphate
Reference proteome
Sphingolipid metabolism
Transferase
Transmembrane
Transmembrane helix
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9862192] Weiser, Joel, 2024-02-26
[InstanceEdit:9917590] Weiser, Joel, 2024-08-09
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
nameSPTLC3
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:8993331] ENSEMBL:ENSG00000172296 SPTLC3 [Homo sapiens]
secondaryIdentifierSPTC3_HUMAN
A2A2I4
B9EK64
Q05DQ8
Q5T1U4
Q9H1L1
Q9H1Z0
sequenceLength552
species[Species:48887] Homo sapiens
(isoformParent)[ReferenceIsoform:249307] UniProt:Q9NUV7-2 SPTLC3 [Homo sapiens]
[ReferenceIsoform:414807] UniProt:Q9NUV7-1 SPTLC3 [Homo sapiens]
(referenceEntity)[EntityWithAccessionedSequence:428162] PXLP-K371-SPTLC3 [endoplasmic reticulum membrane] [Homo sapiens]
(referenceSequence)[ModifiedResidue:554222] N6-pyridoxal phosphate-L-lysine at 371
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