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Details on Person UniProt:D3ZKX9 Aloxe3

Class:IdReferenceGeneProduct:5083149
_chainChangeLogchain:1-711 added on Fri February 6 2015
_displayNameUniProt:D3ZKX9 Aloxe3
_timestamp2025-02-21 19:39:36
chainchain:1-711
checksum41C3D9BBC6C4532B
commentFUNCTION Non-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced lipoxygenases activity. The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols and ketones. In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides. In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites (By similarity). Also plays a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activate PPARG (By similarity). Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia (PubMed:23382512).CATALYTIC ACTIVITY a hydroperoxyeicosatetraenoate = a hydroxy-epoxy-eicosatetraenoateCATALYTIC ACTIVITY a hydroperoxyeicosatetraenoate = an oxoeicosatetraenoate + H2OCATALYTIC ACTIVITY (12R)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate = (8R)-hydroxy-(11R,12R)-epoxy-(5Z,9E,14Z)-eicosatrienoateCATALYTIC ACTIVITY (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate = (8R)-hydroxy-(11S,12S)-epoxy-(5Z,9E,14Z)-eicosatrienoateCATALYTIC ACTIVITY (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate = (10R)-hydroxy-(11S,12S)-epoxy-(5Z,8Z,14Z)-eicosatrienoateCATALYTIC ACTIVITY (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate = (13R)-hydroxy-(14S,15S)-epoxy-(5Z,8Z,11Z)-eicosatrienoateCATALYTIC ACTIVITY (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate = 7R-hydroxy-5S,6S-epoxy-(8Z,11Z,14Z)-eicosatrienoateCATALYTIC ACTIVITY (13S)-hydroperoxy-(9Z,11E)-octadecadienoate = 11-hydroxy-(12S,13S)-epoxy-(9Z)-octadecenoateCATALYTIC ACTIVITY N-[omega-(9R)-hydroperoxy-(10E,12Z)-octadecadienoyloxy]acyl-beta-D-glucosyl-(1<->1)-octadecasphing-4E-enine = a N-[omega-(9R,10R)-epoxy-(13R)-hydroxy-(11E)-octadecenoyloxy]acyl-beta-D-glucosyl-(1<->1)-sphing-4E-enineCATALYTIC ACTIVITY a N-[omega-(9R)-hydroperoxy-(10E,12Z)-octadecadienoyloxy]-acylsphin-4E-enine = a N-[omega-(9R,10R)-epoxy-(13R)-hydroxy-(11E)-octadecenoyloxy]-acylsphing-4E-enineCATALYTIC ACTIVITY (12R)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate = 12-oxo-(5Z,8Z,10E,14Z)-eicosatetraenoate + H2OCATALYTIC ACTIVITY (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate = 12-oxo-(5Z,8Z,10E,14Z)-eicosatetraenoate + H2OCATALYTIC ACTIVITY (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate = 15-oxo-(5Z,8Z,11Z,13E)-eicosatetraenoate + H2OCATALYTIC ACTIVITY (13S)-hydroperoxy-(9Z,11E)-octadecadienoate = 13-oxo-(9Z,11E)-octadecadienoate + H2OCATALYTIC ACTIVITY (8S)-hydroperoxy-(5Z,9E,11Z,14Z)-eicosatetraenoate = (10R)-hydroxy-(8S,9S)-epoxy-(5Z,11Z,14Z)-eicosatrienoateCATALYTIC ACTIVITY (8R)-hydroperoxy-(5Z,9E,11Z,14Z)-eicosatetraenoate = 8-oxo-(5Z,9E,11Z,14Z)-eicosatetraenoate + H2OCATALYTIC ACTIVITY (8S)-hydroperoxy-(5Z,9E,11Z,14Z)-eicosatetraenoate = 8-oxo-(5Z,9E,11Z,14Z)-eicosatetraenoate + H2OCOFACTOR Binds 1 Fe cation per subunit.PATHWAY Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.PATHWAY Lipid metabolism; sphingolipid metabolism.SUBCELLULAR LOCATION Belongs to the lipoxygenase family.ONLINE INFORMATION about water - Issue 153 of September 2013
created[InstanceEdit:5083144] Weiser, JD
descriptionrecommendedName: fullName evidence="6"Hydroperoxide isomerase ALOXE3 alternativeName: fullName evidence="2"Epidermis-type lipoxygenase 3 shortName: Epidermal LOX-3 shortName evidence="2"e-LOX-3 shortName: eLOX-3 alternativeName: fullName evidence="6"Hydroperoxy dehydratase ALOXE3 alternativeName: Hydroperoxy icosatetraenoate dehydratase ecNumber evidence="2"4.2.1.152 alternativeName: Hydroperoxy icosatetraenoate isomerase ecNumber evidence="2"5.4.4.7
geneNameAloxe3
identifierD3ZKX9
isSequenceChangedFALSE
keywordCytoplasm
Dioxygenase
Fatty acid metabolism
Iron
Isomerase
Lipid metabolism
Lyase
Metal-binding
Oxidoreductase
Reference proteome
modified[InstanceEdit:5433710] Weiser, JD
[InstanceEdit:5618415] Weiser, JD
[InstanceEdit:5634237] Weiser, JD
[InstanceEdit:5673015] Weiser, JD
[InstanceEdit:8856987] Weiser, JD
[InstanceEdit:9037114] Weiser, JD
[InstanceEdit:9637257] Weiser, JD
[InstanceEdit:9676415] Weiser, JD
[InstanceEdit:9698430] Weiser, JD
[InstanceEdit:9796772] Weiser, Joel
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
nameAloxe3
referenceDatabase[ReferenceDatabase:2] UniProt
secondaryIdentifierLOXE3_RAT
sequenceLength711
species[Species:48895] Rattus norvegicus
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