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Details on Person UniProt:Q96KQ7 EHMT2

Class:IdReferenceGeneProduct:50713
_chainChangeLoginitiator methionine:1 added on Fri February 6 2015;chain:2-1210 added on Fri February 6 2015;initiator methionine:1 for 50713 removed on Fri Nov 03 2023;initiator methionine: for 50713 added on Fri Nov 03 2023;initiator methionine: for 50713 removed on Fri Aug 15 2025;initiator methionine:1 for 50713 added on Fri Aug 15 2025
_displayNameUniProt:Q96KQ7 EHMT2
_timestamp2026-02-20 21:58:40
chaininitiator methionine:1
chain:2-1210
checksumA6C5DC6B72801520
commentFUNCTION Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones (PubMed:11316813, PubMed:20084102). Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication (PubMed:22387026). Also weakly methylates 'Lys-27' of histone H3 (H3K27me) (PubMed:11316813). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also able to mono- and dimethylate histone H1-4 at 'Lys-26' (H1.4K26me1 and H1.4K26me2, respectively) (PubMed:19144645). In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53 (PubMed:20118233). Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself (PubMed:18438403).CATALYTIC ACTIVITY N(6)-methyl-L-lysyl(9)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(9)-[histone H3] + S-adenosyl-L-homocysteine + H(+)CATALYTIC ACTIVITY L-lysyl(9)-[histone H3] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl(9)-[histone H3] + S-adenosyl-L-homocysteine + H(+)CATALYTIC ACTIVITY L-lysyl(56)-[histone H3] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl(56)-[histone H3] + S-adenosyl-L-homocysteine + H(+)CATALYTIC ACTIVITY N(6)-methyl-L-lysyl(56)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(56)-[histone H3] + S-adenosyl-L-homocysteine + H(+)SUBUNIT Heterodimer; heterodimerizes with EHMT1/GLP (PubMed:16702210). Interacts with GFI1B and WIZ (PubMed:16688220, PubMed:16702210). Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EHMT1, RING1, RNF2, MBLR, L3MBTL2 and YAF2 (PubMed:12004135). Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2 (PubMed:21549307). Interacts with UHRF1 (PubMed:19056828). Interacts with CDYL (PubMed:19061646). Interacts with REST only in the presence of CDYL (PubMed:19061646). Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2 (PubMed:19061646). Interacts with PRDM9 and CDYL; interaction only takes place when PRDM9 is bound to hotspot DNA (By similarity). Interacts with SMYD5 (By similarity).INTERACTION Associates with euchromatic regions (PubMed:11316813). Does not associate with heterochromatin (PubMed:11316813).ALTERNATIVE PRODUCTS Additional isoforms seem to exist.TISSUE SPECIFICITY Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.DOMAIN The SET domain mediates interaction with WIZ.DOMAIN The ANK repeats bind H3K9me1 and H3K9me2.DOMAIN In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.PTM Methylated at Lys-185; automethylated.SIMILARITY Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.CAUTION While NG36 and G9a were originally thought to derive from 2 separate genes, all G9A transcripts also contain the in frame coding sequence of NG36.CAUTION It is uncertain whether Met-1 or Met-21 is the initiator methionine.SEQUENCE CAUTION Extended N-terminus.SEQUENCE CAUTION Extended N-terminus.SEQUENCE CAUTION Truncated N-terminus.SEQUENCE CAUTION Extended N-terminus.SEQUENCE CAUTION Truncated N-terminus.
descriptionrecommendedName: Histone-lysine N-methyltransferase EHMT2 ecNumber evidence="6 18 21"2.1.1.- ecNumber evidence="6 18"2.1.1.367 alternativeName: Euchromatic histone-lysine N-methyltransferase 2 alternativeName: HLA-B-associated transcript 8 alternativeName: Histone H3-K9 methyltransferase 3 shortName: H3-K9-HMTase 3 alternativeName: Lysine N-methyltransferase 1C alternativeName: Protein G9a
geneNameEHMT2
BAT8
C6orf30
G9A
KMT1C
NG36
identifierQ96KQ7
isSequenceChangedFALSE
keyword3D-structure
Acetylation
Alternative splicing
ANK repeat
Chromatin regulator
Chromosome
Isopeptide bond
Metal-binding
Methylation
Methyltransferase
Nucleus
Phosphoprotein
Proteomics identification
Reference proteome
Repeat
S-adenosyl-L-methionine
Transferase
Ubl conjugation
Zinc
modified[InstanceEdit:84067] Schmidt, EE, 2003-12-18 04:29:09
[InstanceEdit:143527] Schmidt, EE, 2004-11-12 07:45:10
[InstanceEdit:217385] Schmidt, EE, 2008-03-27 06:23:53
[InstanceEdit:354386] Schmidt, EE, 2008-06-18 04:45:12
[InstanceEdit:384350] Kanapin, AA, 2008-11-26 14:00:39
[InstanceEdit:392885] Kanapin, AA, 2009-03-09 12:07:18
[InstanceEdit:400710] Schmidt, EE, 2009-03-25 05:33:35
[InstanceEdit:423310] Kanapin, AA
[InstanceEdit:435478] Kanapin, AA
[InstanceEdit:435871] Kanapin, AA
[InstanceEdit:447347] Kanapin, AA
[InstanceEdit:525883] Kanapin, AA
[InstanceEdit:613449] Kanapin, AA
[InstanceEdit:797602] Kanapin, AA
[InstanceEdit:937368] Yung, CK
[InstanceEdit:1042053] Yung, CK
[InstanceEdit:1220657] Yung, CK
[InstanceEdit:1300696] Yung, CK
[InstanceEdit:1301627] Yung, CK
[InstanceEdit:1551960] Weiser, JD
[InstanceEdit:1995863] Weiser, JD
[InstanceEdit:2132304] Weiser, JD
[InstanceEdit:2265580] Weiser, JD
[InstanceEdit:2455454] Weiser, JD
[InstanceEdit:2587579] Weiser, JD
[InstanceEdit:3445779] Weiser, JD
[InstanceEdit:4341137] Weiser, JD
[InstanceEdit:5263031] Weiser, JD
[InstanceEdit:5433710] Weiser, JD
[InstanceEdit:5618415] Weiser, JD
[InstanceEdit:5634237] Weiser, JD
[InstanceEdit:5673015] Weiser, JD
[InstanceEdit:8987656] Weiser, JD
[InstanceEdit:9027688] Weiser, JD
[InstanceEdit:9037114] Weiser, JD
[InstanceEdit:9637257] Weiser, JD
[InstanceEdit:9666080] Weiser, JD
[InstanceEdit:9676415] Weiser, JD
[InstanceEdit:9715482] Weiser, JD
[InstanceEdit:9730071] Weiser, JD
[InstanceEdit:9750299] Weiser, JD
[InstanceEdit:9767224] Weiser, Joel
[InstanceEdit:9773244] Weiser, Joel
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9862192] Weiser, Joel, 2024-02-26
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
[InstanceEdit:9963647] Weiser, Joel, 2025-08-15
[InstanceEdit:9983091] Weiser, Joel, 2026-02-20
nameEHMT2
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:8997681] ENSEMBL:ENSG00000204371 EHMT2 [Homo sapiens]
secondaryIdentifierEHMT2_HUMAN
B0UZY2
Q14349
Q5JP83
Q5JQ92
Q5JQA1
Q5JQG3
Q6PK06
Q96MH5
Q96QD0
Q9UQL8
Q9Y331
sequenceLength1210
species[Species:48887] Homo sapiens
(isoformParent)[ReferenceIsoform:144491] UniProt:Q96KQ7-2 EHMT2 [Homo sapiens]
[ReferenceIsoform:144492] UniProt:Q96KQ7-3 EHMT2 [Homo sapiens]
[ReferenceIsoform:412892] UniProt:Q96KQ7-1 EHMT2 [Homo sapiens]
(referenceEntity)[EntityWithAccessionedSequence:212151] EHMT2 [nucleoplasm] [Homo sapiens]
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No pathways have been reviewed or authored by UniProt:Q96KQ7 EHMT2 (50713)