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Details on Person UniProt:P62331 Arf6

Class:IdReferenceGeneProduct:50247
_chainChangeLoginitiator methionine:1 added on Fri February 6 2015;chain:2-175 added on Fri February 6 2015;initiator methionine:1 for 50247 removed on Fri Nov 03 2023;initiator methionine: for 50247 added on Fri Nov 03 2023;initiator methionine: for 50247 removed on Fri Aug 15 2025;initiator methionine:1 for 50247 added on Fri Aug 15 2025
_displayNameUniProt:P62331 Arf6
_timestamp2025-08-15 21:51:05
chaininitiator methionine:1
chain:2-175
checksum49E38E59AEA52B98
commentFUNCTION GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling (PubMed:11950392). Required for normal completion of mitotic cytokinesis. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization. Regulates surface levels of adherens junction proteins such as CDH1 (PubMed:20080746, PubMed:29420262). Required for NTRK1 sorting to the recycling pathway from early endosomes (By similarity).CATALYTIC ACTIVITY GTP + H2O = GDP + phosphate + H(+)ACTIVITY REGULATION Activation is generally mediated by guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by GTPases activating protein (GAP). Inactivated by ACAP1 and ACAP2 (By similarity). Activated by NGF via NTRK1 (By similarity).SUBUNIT Interacts (when activated) with GGA1, GGA2 and GGA3; the interaction is required for proper subcellular location of GGA1, GGA2 and GGA3 (PubMed:11950392, PubMed:22522702). Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA. Interacts with NCS1/FREQ at the plasma membrane. Interacts with RAB11FIP3. Interacts with USP6 (via Rab-GAP TBC domain). Interacts with ECPAS. Interacts with TBC1D24. Interacts with MICALL1. Interacts with CYTH3 (By similarity). Interacts with KIF23, forming heterodimers and heterotetramers. Interacts with SPAG9 and RAB11FIP4 (PubMed:22522702). Interacts with C9orf72 (PubMed:27723745). Interacts (GTP-bound form) with TJAP1/PILT (PubMed:22841714).INTERACTION Distributed uniformly on the plasma membrane, as well as throughout the cytoplasm during metaphase (By similarity). Subsequently concentrated at patches in the equatorial region at the onset of cytokinesis, and becomes distributed in the equatorial region concurrent with cleavage furrow ingression (By similarity). In late stages of cytokinesis, concentrates at the midbody ring/Flemming body (By similarity). Recruitment to the midbody ring requires both activation by PSD/EFA6A and interaction with KIF23/MKLP1 (By similarity). After abscission of the intercellular bridge, incorporated into one of the daughter cells as a midbody remnant and localizes to punctate structures beneath the plasma membrane (PubMed:22522702). Recruited to the cell membrane in association with CYTH2 and ARL4C (By similarity). Colocalizes with DAB2IP at the plasma membrane and endocytic vesicles (By similarity). Myristoylation is required for proper localization to membranes: myristoylation on Lys-3 allows ARF6 to remain on membranes during the GTPase cycle (By similarity).TISSUE SPECIFICITY Widely expressed.PTM GTP-bound form is myristoylated on Lys-3 by NMT1 and NMT2, allowing ARF6 to remain on membranes during the GTPase cycle, thereby promoting its activity. GDP-bound inactive form is demyristoylated on Lys-3 by SIRT2 at early endosomes or endocytic recycling compartment to allow its efficient activation by a guanine exchange factor (GEF) after GDP release.SIMILARITY Belongs to the small GTPase superfamily. Arf family.
descriptionrecommendedName: ADP-ribosylation factor 6 ecNumber evidence="1"3.6.5.2
geneNameArf6
identifierP62331
isSequenceChangedFALSE
keyword3D-structure
Cell cycle
Cell division
Cell membrane
Cell projection
Cytoplasm
Differentiation
Endosome
Golgi apparatus
GTP-binding
Hydrolase
Lipoprotein
Membrane
Myristate
Neurogenesis
Nucleotide-binding
Protein transport
Reference proteome
Transport
modified[InstanceEdit:84067] Schmidt, EE, 2003-12-18 04:29:09
[InstanceEdit:143527] Schmidt, EE, 2004-11-12 07:45:10
[InstanceEdit:217385] Schmidt, EE, 2008-03-27 06:23:53
[InstanceEdit:354386] Schmidt, EE, 2008-06-18 04:45:12
[InstanceEdit:384350] Kanapin, AA, 2008-11-26 14:00:39
[InstanceEdit:392885] Kanapin, AA, 2009-03-09 12:07:18
[InstanceEdit:400710] Schmidt, EE, 2009-03-25 05:33:35
[InstanceEdit:423310] Kanapin, AA
[InstanceEdit:435478] Kanapin, AA
[InstanceEdit:435871] Kanapin, AA
[InstanceEdit:447347] Kanapin, AA
[InstanceEdit:525883] Kanapin, AA
[InstanceEdit:613449] Kanapin, AA
[InstanceEdit:797602] Kanapin, AA
[InstanceEdit:937368] Yung, CK
[InstanceEdit:1042053] Yung, CK
[InstanceEdit:1220657] Yung, CK
[InstanceEdit:1300696] Yung, CK
[InstanceEdit:1301627] Yung, CK
[InstanceEdit:1551960] Weiser, JD
[InstanceEdit:1995863] Weiser, JD
[InstanceEdit:2132304] Weiser, JD
[InstanceEdit:2265580] Weiser, JD
[InstanceEdit:2455454] Weiser, JD
[InstanceEdit:5263031] Weiser, JD
[InstanceEdit:5433710] Weiser, JD
[InstanceEdit:5618415] Weiser, JD
[InstanceEdit:5634237] Weiser, JD
[InstanceEdit:5673015] Weiser, JD
[InstanceEdit:9037114] Weiser, JD
[InstanceEdit:9607352] Weiser, JD
[InstanceEdit:9627708] Weiser, JD
[InstanceEdit:9637257] Weiser, JD
[InstanceEdit:9657908] Weiser, JD
[InstanceEdit:9676415] Weiser, JD
[InstanceEdit:9730071] Weiser, JD
[InstanceEdit:9796772] Weiser, Joel
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9917590] Weiser, Joel, 2024-08-09
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
[InstanceEdit:9963647] Weiser, Joel, 2025-08-15
nameArf6
referenceDatabase[ReferenceDatabase:2] UniProt
secondaryIdentifierARF6_MOUSE
P26438
sequenceLength175
species[Species:48892] Mus musculus
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No pathways have been reviewed or authored by UniProt:P62331 Arf6 (50247)