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Details on Person UniProt:P84078 Arf1
| Class:Id | ReferenceGeneProduct:50222 |
|---|---|
| _chainChangeLog | initiator methionine:1 added on Fri February 6 2015;chain:2-181 added on Fri February 6 2015;initiator methionine:1 for 50222 removed on Fri Nov 03 2023;initiator methionine: for 50222 added on Fri Nov 03 2023;initiator methionine: for 50222 removed on Fri Aug 15 2025;initiator methionine:1 for 50222 added on Fri Aug 15 2025 |
| _displayName | UniProt:P84078 Arf1 |
| _timestamp | 2025-08-15 21:28:21 |
| chain | initiator methionine:1 chain:2-181 |
| checksum | AAC773D4A60186B6 |
| comment | FUNCTION Small GTPase involved in protein trafficking between different compartments (PubMed:11950392). Modulates vesicle budding and uncoating within the Golgi complex (By similarity). In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane (By similarity). The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles (By similarity). The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD) (By similarity). Plays a key role in the regulation of intestinal stem cells and gut microbiota, and is essential for maintaining intestinal homeostasis (PubMed:36566324). Also plays a critical role in mast cell expansion but not in mast cell maturation by facilitating optimal mTORC1 activation (PubMed:37392777).CATALYTIC ACTIVITY GTP + H2O = GDP + phosphate + H(+)ACTIVITY REGULATION Alternates between an inactive GDP-bound form and an active GTP-bound form (By similarity). Activated by guanine nucleotide-exchange factors (GEFs) and inactivated by GTPase-activating proteins (GAPs) (By similarity).SUBUNIT Interacts (when activated) with GGA1, GGA2 and GGA3; the interaction is required for proper subcellular location of GGA1, GGA2 and GGA3 (PubMed:11950392, PubMed:12679809). Interacts with ARHGAP21, ASAP2, HERC1, PRKCABP, PIP5K1B, TMED2, PSCD2, TMED10 and GRIA2 (PubMed:17347647). Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Interacts with PI4KB in the Golgi complex. Interacts with NCS1/FREQ in the Golgi and at the plasma membrane. Interacts with PLEKHA3. Interacts with PLEKHA8; the interaction, together with phosphatidylinositol 4-phosphate binding, is required for FAPP2-mediated glucosylceramide transfer activity. Interacts (activated) with PICK1 (via PDZ domain); the interaction blocks Arp2/3 complex inhibition. Interacts with IQSEC1 (By similarity). Interacts with C9orf72 (By similarity) (PubMed:27723745).INTERACTION In the GDP-bound form, associates transiently with the membranes via its myristoylated N-terminus where guanine nucleotide-exchange factor (GEF)-mediated nucleotide exchange occurs (By similarity). Following nucleotide exchange, the GTP-bound form undergoes a conformational change, leading to the exposure of a myristoylated N-terminal amphipathic helix that provides stable membrane anchorage (By similarity).DISRUPTION PHENOTYPE In intestine cells, ARF1 deletion results in a decreased number of proliferative cells as well as an increase in the number of apoptotic epithelial cells in the crypt areas. In addition, crypts from control mice survive and increase in size while isolated crypts from ARF1 knockout mice display an clear reduction in organoid-forming capacity (PubMed:37392777). Loss of ARF1 in bone marrow-derived mast cells attenuates mast cell expansion (PubMed:36566324).SIMILARITY Belongs to the small GTPase superfamily. Arf family. |
| description | recommendedName: ADP-ribosylation factor 1 ecNumber evidence="1"3.6.5.2 |
| geneName | Arf1 |
| identifier | P84078 |
| isSequenceChanged | FALSE |
| keyword | 3D-structure Acetylation Direct protein sequencing ER-Golgi transport Golgi apparatus GTP-binding Hydrolase Lipoprotein Membrane Myristate Nucleotide-binding Protein transport Reference proteome Synapse Synaptosome Transport |
| modified | [InstanceEdit:84067] Schmidt, EE, 2003-12-18 04:29:09 [InstanceEdit:143527] Schmidt, EE, 2004-11-12 07:45:10 [InstanceEdit:217385] Schmidt, EE, 2008-03-27 06:23:53 [InstanceEdit:354386] Schmidt, EE, 2008-06-18 04:45:12 [InstanceEdit:384350] Kanapin, AA, 2008-11-26 14:00:39 [InstanceEdit:392885] Kanapin, AA, 2009-03-09 12:07:18 [InstanceEdit:400710] Schmidt, EE, 2009-03-25 05:33:35 [InstanceEdit:423310] Kanapin, AA [InstanceEdit:435478] Kanapin, AA [InstanceEdit:435871] Kanapin, AA [InstanceEdit:447347] Kanapin, AA [InstanceEdit:525883] Kanapin, AA [InstanceEdit:613449] Kanapin, AA [InstanceEdit:797602] Kanapin, AA [InstanceEdit:937368] Yung, CK [InstanceEdit:1042053] Yung, CK [InstanceEdit:1220657] Yung, CK [InstanceEdit:1300696] Yung, CK [InstanceEdit:1301627] Yung, CK [InstanceEdit:1551960] Weiser, JD [InstanceEdit:1995863] Weiser, JD [InstanceEdit:2132304] Weiser, JD [InstanceEdit:2265580] Weiser, JD [InstanceEdit:2587579] Weiser, JD [InstanceEdit:3445779] Weiser, JD [InstanceEdit:4341137] Weiser, JD [InstanceEdit:5263031] Weiser, JD [InstanceEdit:5433710] Weiser, JD [InstanceEdit:5618415] Weiser, JD [InstanceEdit:5634237] Weiser, JD [InstanceEdit:5673015] Weiser, JD [InstanceEdit:9037114] Weiser, JD [InstanceEdit:9637257] Weiser, JD [InstanceEdit:9657908] Weiser, JD [InstanceEdit:9676415] Weiser, JD [InstanceEdit:9796772] Weiser, Joel [InstanceEdit:9819394] Weiser, Joel [InstanceEdit:9852000] Weiser, Joel, 2023-11-03 [InstanceEdit:9862192] Weiser, Joel, 2024-02-26 [InstanceEdit:9917590] Weiser, Joel, 2024-08-09 [InstanceEdit:9939033] Weiser, Joel, 2025-02-21 [InstanceEdit:9948485] Weiser, Joel, 2025-05-21 [InstanceEdit:9963647] Weiser, Joel, 2025-08-15 |
| name | Arf1 |
| referenceDatabase | [ReferenceDatabase:2] UniProt |
| secondaryIdentifier | ARF1_MOUSE P10947 P32889 Q3THZ2 Q3U849 Q3UDG1 Q3UF76 |
| sequenceLength | 181 |
| species | [Species:48892] Mus musculus |
| [Change default viewing format] | |
No pathways have been reviewed or authored by UniProt:P84078 Arf1 (50222)
