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Details on Person UniProt:Q9Y4C5-1 CHST2

Class:IdReferenceIsoform:416015
_chainChangeLogchain:1-530 added on Sat February 7 2015
_displayNameUniProt:Q9Y4C5-1 CHST2
_timestamp2026-02-20 22:53:03
chainchain:1-530
checksumA82CA227B9D5651B
commentFUNCTION Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N- and O-linked glycans and within O-linked mucin-type glycans (PubMed:11042394, PubMed:11726653, PubMed:35939855, PubMed:38034954, PubMed:9722682). Selectively transfers the sulfate group onto the terminal GlcNAc of alpha1,3-Man or alpha1,6-Man antenna of complex-type N-glycans depending on glycan composition. Only sulfates terminal GlcNAc of alpha1,3-Man antenna of G0 complex-type N-glycans. Can sulfate keratan-type N-acetyllactosamine (LacNAc) repeats generating epitopes for self versus non-self immune recognition by C-type lectins (PubMed:35939855, PubMed:38034954). Transfers the sulfate group primarily on core 2 GlcNAcbeta1-6(Galbeta1-3)GalNAcalpha-Ser/Thr and with lower efficiency on extended core 1 GlcNAcbeta1-3Galbeta1-3GalNAcalpha-Ser/Thr based O-linked glycans on peripheral node addressins (PNAds) expressed on the lumenal side of high endothelial venules (HEVs). Shares substrate specificity with CHST4 and both contribute to generate sialyl 6-sulfo Lewis X determinant (also known as MECA-79 epitope) for SELL recognition, a prerequisite for continuous lymphocyte homing into peripheral lymph nodes and antigen immune surveillance (By similarity) (PubMed:9722682). Has no activity toward alpha-linked GlcNAc moiety exposed at the non-reducing ends or to internally located GlcNAc residues (PubMed:11042394).CATALYTIC ACTIVITY an N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative + 3'-phosphoadenylyl sulfate = a 6-O-sulfo-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative + adenosine 3',5'-bisphosphate + H(+)CATALYTIC ACTIVITY an N(4)-{alpha-Neu5Ac-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + 3'-phosphoadenylyl sulfate = an N(4)-{alpha-Neu5Ac-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[6-O-sulfo-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + adenosine 3',5'-bisphosphate + H(+)CATALYTIC ACTIVITY N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + 3'-phosphoadenylyl sulfate = an N(4)-{6-O-sulfo-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L--asparaginyl-[protein] + adenosine 3',5'-bisphosphate + H(+)CATALYTIC ACTIVITY a 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] + 3'-phosphoadenylyl sulfate = 3-O-{beta-D-galactosyl-(1->3)-[6-O-sulfo-N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] + adenosine 3',5'-bisphosphate + H(+)CATALYTIC ACTIVITY 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + 3'-phosphoadenylyl sulfate = 3-O-{beta-D-galactosyl-(1->3)-[6-O-sulfo-N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + adenosine 3',5'-bisphosphate + H(+)CATALYTIC ACTIVITY 3-O-{N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] + 3'-phosphoadenylyl sulfate = 3-O-{6-O-sulfo-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] + adenosine 3',5'-bisphosphate + H(+)CATALYTIC ACTIVITY 3-O-{N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + 3'-phosphoadenylyl sulfate = 3-O-{6-O-sulfo-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + adenosine 3',5'-bisphosphate + H(+)BIOPHYSICOCHEMICAL PROPERTIES Protein modification; carbohydrate sulfation.SUBUNIT Homodimer; disulfide-linked. Homodimerization is not essential for enzyme activity.SUBCELLULAR LOCATION Widely expressed. Highly expressed in bone marrow, peripheral blood leukocytes, spleen, brain, spinal cord, ovary and placenta. Expressed by high endothelial cells (HEVs) and leukocytes.INDUCTION Up-regulated upon cytokine activation.PTM Glycosylation at Asn-475 is required for catalytic activity.MISCELLANEOUS Higher levels of expression compared to isoform 1 when expressed in HeLa cells. Exhibits similar intracellular GlcNAc-6-O-sulfation activity.SIMILARITY Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
created[InstanceEdit:400710] Schmidt, EE, 2009-03-25 05:33:35
descriptionrecommendedName: Carbohydrate sulfotransferase 2 ecNumber evidence="7"2.8.2.- alternativeName: Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2 shortName: GST-2 alternativeName: N-acetylglucosamine 6-O-sulfotransferase 1 shortName evidence="17"GlcNAc6ST-1 shortName: Gn6ST-1
geneNameCHST2
GN6ST
identifierQ9Y4C5
isoformParent
isSequenceChangedFALSE
keywordAlternative initiation
Carbohydrate metabolism
Disulfide bond
Glycoprotein
Golgi apparatus
Inflammatory response
Membrane
Proteomics identification
Reference proteome
Signal-anchor
Transferase
Transmembrane
Transmembrane helix
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
[InstanceEdit:9983091] Weiser, Joel, 2026-02-20
nameCHST2
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:8990597] ENSEMBL:ENSG00000175040 CHST2 [Homo sapiens]
secondaryIdentifierCHST2_HUMAN
D3DNG5
Q2M370
Q9GZN5
Q9UED5
Q9Y6F2
sequenceLength530
species[Species:48887] Homo sapiens
variantIdentifierQ9Y4C5-1
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No pathways have been reviewed or authored by UniProt:Q9Y4C5-1 CHST2 (416015)
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