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Details on Person UniProt:Q07157 TJP1

Class:IdReferenceGeneProduct:405236
_chainChangeLogchain:1-1748 added on Fri February 6 2015
_displayNameUniProt:Q07157 TJP1
_timestamp2025-02-21 19:03:40
chainchain:1-1748
checksum189E31617084C0CC
commentFUNCTION TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ (By similarity). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed:21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed:20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions (By similarity).SUBUNIT Homodimer (PubMed:16737969, PubMed:17928286). Forms heterodimers TJP3 (By similarity). Forms a heterodimer (via PDZ2 domain) with TJP2/ZO2 (via PDZ2 domain) (PubMed:17897942, PubMed:9792688). Interacts with OCLN, CALM, claudins, CGN/cingulin, CXADR, GJA12, GJD3 and UBN1 (PubMed:11734628, PubMed:12023291, PubMed:12154091, PubMed:15183511, PubMed:18823282, PubMed:20200156, PubMed:7798316). Interacts (via ZU5 domain) with CDC42BPB and MYZAP (PubMed:20093627, PubMed:21240187). Interacts (via PDZ domain) with GJA1 (PubMed:18636092). Interacts (via PDZ domains) with ANKRD2 (PubMed:22016770). Interacts with POPDC1 (via the C-terminus cytoplasmic tail) (By similarity). Interacts with HSPA4 and KIRREL1 (By similarity). Interacts with DLL1 (By similarity). Interacts with USP53 (via the C-terminal region) (By similarity). Interacts (via ABR region) with F-actin (PubMed:12354695, PubMed:20930113, PubMed:9792688). Interacts with DNMBP (via C-terminal domain); required for the apical cell-cell junction localization of DNMBP (PubMed:17015620). Interacts with SPEF1 (PubMed:31473225). Interacts (via N-terminus) with CTNNA1 (By similarity). Interacts with CLDN18 (By similarity). Interacts with CLDN16 (via TRV motif); this is a prerequisite for anchoring of CLDN16 at the tight junction. Interacts with PKP1; the interaction facilitates TJP1/ZO-1 localization to the plasma membrane (By similarity). Interacts with PATJ (via PDZ1-6 domains); the interaction is required for attachment and extension of TJP1/ZO1 condensates along the apical cell interface (By similarity).INTERACTION Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact (PubMed:7798316). Forms a condensed tight junction-linked belt of protein during junction formation which becomes anchored to the apical cell membrane via interaction with PATJ (By similarity). At podosomal sites, is predominantly localized in the ring structure surrounding the actin core (PubMed:20930113). Colocalizes with SPEF1 at sites of cell-cell contact in intestinal epithelial cells (PubMed:31473225).ALTERNATIVE PRODUCTS The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.DOMAIN The 244-aa domain between residues 633 and 876 is the primary occludin (OCLN)-binding site and is required for stable association with the tight junction (PubMed:9792688).DOMAIN The C-terminal region (residues 1151-1372) is an actin-binding region (ABR) that interacts directly with F-actin and plays an important role in the localization of TJP1 at junctions (PubMed:12354695, PubMed:20930113, PubMed:9792688). The ABR is also required for the localization to puncta at the free edge of cells before initiation of cell-cell contact (PubMed:12354695). The ABR is also necessary for TJP1 recruitment to podosomes (PubMed:20930113).DOMAIN The second PDZ domain (PDZ2) mediates homodimerization and heterodimerization with TJP2 and TJP3 (PubMed:17928286, PubMed:9792688).PTM Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF) (PubMed:19332538, PubMed:7542259). This response is dependent on an intact actin microfilament system (PubMed:7542259). Dephosphorylated by PTPRJ (PubMed:19332538).SIMILARITY Belongs to the MAGUK family.SEQUENCE CAUTION Truncated N-terminus.
created[InstanceEdit:400710] Schmidt, EE, 2009-03-25 05:33:35
descriptionrecommendedName: fullName evidence="36"Tight junction protein 1 alternativeName: fullName evidence="34"Tight junction protein ZO-1 alternativeName: Zona occludens protein 1 alternativeName: Zonula occludens protein 1
geneNameTJP1
ZO1
identifierQ07157
isSequenceChangedFALSE
keyword3D-structure
Alternative splicing
Calmodulin-binding
Cell junction
Cell membrane
Cell projection
Gap junction
Membrane
Phosphoprotein
Proteomics identification
Reference proteome
Repeat
SH3 domain
Tight junction
modified[InstanceEdit:423310] Kanapin, AA
[InstanceEdit:435478] Kanapin, AA
[InstanceEdit:435871] Kanapin, AA
[InstanceEdit:447347] Kanapin, AA
[InstanceEdit:525883] Kanapin, AA
[InstanceEdit:613449] Kanapin, AA
[InstanceEdit:797602] Kanapin, AA
[InstanceEdit:937368] Yung, CK
[InstanceEdit:1042053] Yung, CK
[InstanceEdit:1220657] Yung, CK
[InstanceEdit:1300696] Yung, CK
[InstanceEdit:1301627] Yung, CK
[InstanceEdit:1551960] Weiser, JD
[InstanceEdit:1995863] Weiser, JD
[InstanceEdit:2132304] Weiser, JD
[InstanceEdit:2265580] Weiser, JD
[InstanceEdit:5263031] Weiser, JD
[InstanceEdit:5433710] Weiser, JD
[InstanceEdit:5618415] Weiser, JD
[InstanceEdit:5634237] Weiser, JD
[InstanceEdit:5673015] Weiser, JD
[InstanceEdit:8987656] Weiser, JD
[InstanceEdit:9037114] Weiser, JD
[InstanceEdit:9637257] Weiser, JD
[InstanceEdit:9645058] Weiser, JD
[InstanceEdit:9657908] Weiser, JD
[InstanceEdit:9676415] Weiser, JD
[InstanceEdit:9698430] Weiser, JD
[InstanceEdit:9715482] Weiser, JD
[InstanceEdit:9773244] Weiser, Joel
[InstanceEdit:9834092] Weiser, Joel
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9917590] Weiser, Joel, 2024-08-09
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
nameTJP1
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:8935962] ENSEMBL:ENSG00000104067 TJP1 [Homo sapiens]
secondaryIdentifierZO1_HUMAN
B4E3K1
Q2NKP3
Q4ZGJ6
sequenceLength1748
species[Species:48887] Homo sapiens
(isoformParent)[ReferenceIsoform:68286] UniProt:Q07157-1 TJP1 [Homo sapiens]
[ReferenceIsoform:156407] UniProt:Q07157-2 TJP1 [Homo sapiens]
(referenceEntity)[EntityWithAccessionedSequence:532659] TJP1 [cytosol] [Homo sapiens]
[EntityWithAccessionedSequence:3209843] TJP1 [Golgi-associated vesicle membrane] [Homo sapiens]
[EntityWithAccessionedSequence:3209844] TJP1 (?-?) [plasma membrane] [Homo sapiens]
[EntityWithAccessionedSequence:3209845] TJP1 [plasma membrane] [Homo sapiens]
[EntityWithAccessionedSequence:3209852] TJP1 [peroxisomal membrane] [Homo sapiens]
[EntityWithAccessionedSequence:8936023] TJP1 [cell junction] [Homo sapiens]
[EntityWithAccessionedSequence:9921246] TJP1 [nucleoplasm] [Homo sapiens]
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No pathways have been reviewed or authored by UniProt:Q07157 TJP1 (405236)