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Details on Person UniProt:P29475 NOS1
| Class:Id | ReferenceGeneProduct:403575 |
|---|---|
| _chainChangeLog | chain:1-1434 added on Sat February 7 2015 |
| _displayName | UniProt:P29475 NOS1 |
| _timestamp | 2025-02-21 18:59:25 |
| chain | chain:1-1434 |
| checksum | 99235793B953BF37 |
| comment | FUNCTION Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.CATALYTIC ACTIVITY 2 L-arginine + 3 NADPH + 4 O2 + H(+) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H2OCOFACTOR Binds 1 FAD.COFACTOR Binds 1 FMN.COFACTOR Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.ACTIVITY REGULATION Stimulated by calcium/calmodulin. Inhibited by DYNLL1 that prevents the dimerization of the protein. Inhibited by NOSIP.SUBUNIT Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location (By similarity). Interacts with HTR4 (By similarity). Interacts with SLC6A4. Interacts with VAC14 (By similarity). Interacts (via N-terminal domain) with DLG4 (via N-terminal tandem pair of PDZ domains). Interacts with SLC6A4. Forms a complex with ASL, ASS1 and SLC7A1; the complex regulates cell-autonomous L-arginine synthesis and citrulline recycling while channeling extracellular L-arginine to nitric oxide synthesis pathway (By similarity). Interacts with DMD; localizes NOS1 to sarcolemma in muscle cells (By similarity). Interacts with DYNLL1; inhibits the nitric oxide synthase activity (By similarity).INTERACTION In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex (By similarity). In neurons, enriched in dendritic spines (By similarity).ALTERNATIVE PRODUCTS Isoform 3 is produced by different alternative splicing events implicating either the untranslated exons TEX1 (TN-NOS) or TEX1B (TN-NOSB) leading to a N-terminally truncated protein which possesses enzymatic activity comparable to that of isoform 1. The C-terminally truncated isoform 4 is produced by insertion of the TEX2 exon between exons 3 and 4 of isoform 1, leading to a frameshift and a premature stop codon.TISSUE SPECIFICITY Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.DOMAIN The PDZ domain participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes. Mediates interaction with VAC14.PTM Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).SIMILARITY Belongs to the NOS family.ONLINE INFORMATION Nitric oxide synthase entry |
| created | [InstanceEdit:400710] Schmidt, EE, 2009-03-25 05:33:35 |
| description | recommendedName: fullName evidence="17"Nitric oxide synthase 1 ecNumber evidence="9"1.14.13.39 alternativeName: Constitutive NOS alternativeName: NC-NOS alternativeName: NOS type I alternativeName: Neuronal NOS shortName: N-NOS shortName: nNOS alternativeName: fullName evidence="15"Nitric oxide synthase, brain shortName evidence="15"bNOS alternativeName: Peptidyl-cysteine S-nitrosylase NOS1 |
| geneName | NOS1 |
| identifier | P29475 |
| isSequenceChanged | FALSE |
| keyword | 3D-structure Alternative splicing Calmodulin-binding Cell membrane Cell projection FAD Flavoprotein FMN Heme Iron Membrane Metal-binding NADP Oxidoreductase Phosphoprotein Proteomics identification Reference proteome Synapse Ubl conjugation |
| modified | [InstanceEdit:9836292] Weiser, Joel, 2023-05-25 [InstanceEdit:9841277] Weiser, Joel [InstanceEdit:9852000] Weiser, Joel, 2023-11-03 [InstanceEdit:9862192] Weiser, Joel, 2024-02-26 [InstanceEdit:9926675] Weiser, Joel, 2024-11-03 [InstanceEdit:9939033] Weiser, Joel, 2025-02-21 |
| name | NOS1 |
| referenceDatabase | [ReferenceDatabase:2] UniProt |
| referenceGene | [ReferenceDNASequence:9001928] ENSEMBL:ENSG00000089250 NOS1 [Homo sapiens] |
| secondaryIdentifier | NOS1_HUMAN E9PH30 O75713 |
| sequenceLength | 1434 |
| species | [Species:48887] Homo sapiens |
| (isoformParent) | [ReferenceIsoform:60351] UniProt:P29475-1 NOS1 [Homo sapiens] [ReferenceIsoform:151216] UniProt:P29475-2 NOS1 [Homo sapiens] [ReferenceIsoform:151217] UniProt:P29475-3 NOS1 [Homo sapiens] [ReferenceIsoform:151218] UniProt:P29475-4 NOS1 [Homo sapiens] [ReferenceIsoform:8967513] UniProt:P29475-5 NOS1 [Homo sapiens] |
| (referenceEntity) | [EntityWithAccessionedSequence:419293] NOS1 [cytosol] [Homo sapiens] |
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No pathways have been reviewed or authored by UniProt:P29475 NOS1 (403575)
