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Details on Person UniProt:Q90YB1 LIG4

Class:IdReferenceGeneProduct:353309
_chainChangeLogchain:1-912 added on Sat February 7 2015
_displayNameUniProt:Q90YB1 LIG4
_timestamp2024-02-26 03:54:33
chainchain:1-912
checksumCBF2899CA76D212D
commentFUNCTION DNA ligase involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination. Catalyzes the NHEJ ligation step of the broken DNA during DSB repair by resealing the DNA breaks after the gap filling is completed. Joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. LIG4 is mechanistically flexible: it can ligate nicks as well as compatible DNA overhangs alone, while in the presence of XRCC4, it can ligate ends with 2-nucleotides (nt) microhomology and 1-nt gaps. Forms a subcomplex with XRCC4; the LIG4-XRCC4 subcomplex is responsible for the NHEJ ligation step and XRCC4 enhances the joining activity of LIG4.CATALYTIC ACTIVITY ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.COFACTOR Interacts with XRCC4; the LIG4-XRCC4 subcomplex has a 1:2 stoichiometry (By similarity). Component of the core long-range non-homologous end joining (NHEJ) complex (also named DNA-PK complex) composed of PRKDC, LIG4, XRCC4, XRCC6/Ku70, XRCC5/Ku86 and NHEJ1/XLF (By similarity). Additional component of the NHEJ complex includes PAXX (By similarity). Following autophosphorylation, PRKDC dissociates from DNA, leading to formation of the short-range NHEJ complex, composed of LIG4, XRCC4, XRCC6/Ku70, XRCC5/Ku86 and NHEJ1/XLF (By similarity).SUBCELLULAR LOCATION Belongs to the ATP-dependent DNA ligase family.
created[InstanceEdit:353491] Saxena, A, 2008-06-12 16:39:18
descriptionrecommendedName: fullName evidence="7"DNA ligase 4 ecNumber evidence="5"6.5.1.1 alternativeName: fullName evidence="6"DNA ligase IV alternativeName: Polydeoxyribonucleotide synthase [ATP] 4
geneNameLIG4
RCJMB04_10b2
identifierQ90YB1
isSequenceChangedFALSE
keywordATP-binding
Cell cycle
Cell division
DNA damage
DNA recombination
DNA repair
Ligase
Magnesium
Metal-binding
Nucleotide-binding
Nucleus
Reference proteome
Repeat
modified[InstanceEdit:392885] Kanapin, AA, 2009-03-09 12:07:18
[InstanceEdit:400710] Schmidt, EE, 2009-03-25 05:33:35
[InstanceEdit:423310] Kanapin, AA
[InstanceEdit:435478] Kanapin, AA
[InstanceEdit:435871] Kanapin, AA
[InstanceEdit:447347] Kanapin, AA
[InstanceEdit:525883] Kanapin, AA
[InstanceEdit:613449] Kanapin, AA
[InstanceEdit:797602] Kanapin, AA
[InstanceEdit:937368] Yung, CK
[InstanceEdit:1042053] Yung, CK
[InstanceEdit:1220657] Yung, CK
[InstanceEdit:1300696] Yung, CK
[InstanceEdit:1301627] Yung, CK
[InstanceEdit:1551960] Weiser, JD
[InstanceEdit:1995863] Weiser, JD
[InstanceEdit:2132304] Weiser, JD
[InstanceEdit:2265580] Weiser, JD
[InstanceEdit:5433710] Weiser, JD
[InstanceEdit:5618415] Weiser, JD
[InstanceEdit:5634237] Weiser, JD
[InstanceEdit:5673015] Weiser, JD
[InstanceEdit:8964659] Weiser, JD
[InstanceEdit:9037114] Weiser, JD
[InstanceEdit:9637257] Weiser, JD
[InstanceEdit:9676415] Weiser, JD
[InstanceEdit:9765872] Weiser, JD
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9862192] Weiser, Joel, 2024-02-26
nameLIG4
referenceDatabase[ReferenceDatabase:2] UniProt
secondaryIdentifierDNLI4_CHICK
Q5ZKM3
sequenceLength912
species[Species:49591] Gallus gallus
(referenceEntity)[EntityWithAccessionedSequence:353461] DNA ligase IV [nucleoplasm] [Gallus gallus]
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No pathways have been reviewed or authored by UniProt:Q90YB1 LIG4 (353309)