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Details on Person UniProt:Q9Y5B9 SUPT16H

Class:IdReferenceGeneProduct:248920
_chainChangeLoginitiator methionine:1 added on Sat February 7 2015;chain:2-1047 added on Sat February 7 2015;initiator methionine:1 for 248920 removed on Fri Nov 03 2023;initiator methionine: for 248920 added on Fri Nov 03 2023;initiator methionine: for 248920 removed on Fri Aug 15 2025;initiator methionine:1 for 248920 added on Fri Aug 15 2025
_displayNameUniProt:Q9Y5B9 SUPT16H
_timestamp2026-02-20 22:27:07
chaininitiator methionine:1
chain:2-1047
checksum3E1B23C45BDC61C2
commentFUNCTION Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II).SUBUNIT Interacts with MYOG (via C-terminal region) (By similarity). Component of the FACT complex, a stable heterodimer of SSRP1 and SUPT16H (PubMed:10421373). Also a component of a CK2-SPT16-SSRP1 complex which forms following UV irradiation, composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B (PubMed:11239457, PubMed:12393879). Interacts with NEK9 (PubMed:14660563). Binds to histone H2A-H2B (PubMed:10421373). Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1 (PubMed:27499292). Interacts with GTF2E2 (PubMed:10792464).SUBUNIT (Microbial infection) Interacts with Herpes simplex virus 1 (HHV-1) protein ICP22; this interaction relocalizes the FACT complex to viral genomes in infected cells.SUBCELLULAR LOCATION Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.TISSUE SPECIFICITY Ubiquitous.DOMAIN The C-terminal Glu-rich acidic region is essential for FACT activity.PTM ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin.DISEASE The disease may be caused by variants affecting the gene represented in this entry.SIMILARITY Belongs to the peptidase M24 family. SPT16 subfamily.CAUTION Although related to the peptidase M24 family, this protein lacks conserved active site residues suggesting that it may lack peptidase activity.SEQUENCE CAUTION Contaminating sequence. Potential poly-A sequence.SEQUENCE CAUTION Contaminating sequence. Potential poly-A sequence.
created[InstanceEdit:217385] Schmidt, EE, 2008-03-27 06:23:53
descriptionrecommendedName: FACT complex subunit SPT16 alternativeName: Chromatin-specific transcription elongation factor 140 kDa subunit alternativeName: FACT 140 kDa subunit alternativeName: FACTp140 alternativeName: Facilitates chromatin transcription complex subunit SPT16 shortName: hSPT16
geneNameSUPT16H
FACT140
FACTP140
identifierQ9Y5B9
isSequenceChangedFALSE
keyword3D-structure
Acetylation
ADP-ribosylation
Chromosome
Coiled coil
Direct protein sequencing
DNA damage
DNA repair
DNA replication
Host-virus interaction
Intellectual disability
Isopeptide bond
Nucleus
Phosphoprotein
Proteomics identification
Reference proteome
Transcription
Transcription regulation
Ubl conjugation
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9963647] Weiser, Joel, 2025-08-15
[InstanceEdit:9983091] Weiser, Joel, 2026-02-20
nameSUPT16H
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:8988124] ENSEMBL:ENSG00000092201 SUPT16H [Homo sapiens]
secondaryIdentifierSP16H_HUMAN
Q6GMT8
Q6P2F1
Q6PJM1
Q9NRX0
sequenceLength1047
species[Species:48887] Homo sapiens
(referenceEntity)[EntityWithAccessionedSequence:112415] SUPT16H [nucleoplasm] [Homo sapiens]
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