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Details on Person UniProt:Q96RS0 TGS1

Class:IdReferenceGeneProduct:192207
_chainChangeLogchain:1-853 added on Sat February 7 2015
_displayNameUniProt:Q96RS0 TGS1
_timestamp2025-02-21 19:13:30
chainchain:1-853
checksumFF670AFBE0979443
commentFUNCTION Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.CATALYTIC ACTIVITY a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)CATALYTIC ACTIVITY a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)CATALYTIC ACTIVITY a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)CATALYTIC ACTIVITY a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)BIOPHYSICOCHEMICAL PROPERTIES Optimum pH is 8.5-9.5.SUBUNIT May form homooligomers. Interacts with CREBBP/CBP, EED/WAIT1, EP300/P300, NCOA6/PRIP, PPARBP/PBP and SMN.INTERACTION A 90 kDa isoform is found in the nucleus while a 55 kDa isoform is found in the cytoplasm and colocalizes with the tubulin network.TISSUE SPECIFICITY Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta.SIMILARITY Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family.SEQUENCE CAUTION Truncated N-terminus.SEQUENCE CAUTION Truncated N-terminus.
created[InstanceEdit:192208] Gillespie, ME, 2007-01-29 18:19:14
descriptionrecommendedName: Trimethylguanosine synthase ecNumber evidence="11"2.1.1.- alternativeName: CLL-associated antigen KW-2 alternativeName: Cap-specific guanine-N(2) methyltransferase alternativeName: Hepatocellular carcinoma-associated antigen 137 alternativeName: Nuclear receptor coactivator 6-interacting protein alternativeName: PRIP-interacting protein with methyltransferase motif shortName: PIMT shortName: PIPMT
geneNameTGS1
HCA137
NCOA6IP
PIMT
identifierQ96RS0
isSequenceChangedFALSE
keyword3D-structure
Cytoplasm
Methyltransferase
Nucleus
Phosphoprotein
Proteomics identification
Reference proteome
S-adenosyl-L-methionine
Transcription
Transcription regulation
Transferase
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9917590] Weiser, Joel, 2024-08-09
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
nameTGS1
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:8992784] ENSEMBL:ENSG00000137574 TGS1 [Homo sapiens]
secondaryIdentifierTGS1_HUMAN
A6NJQ5
Q5GH23
Q8TDG9
Q96QU3
Q9H5V3
sequenceLength853
species[Species:48887] Homo sapiens
(referenceEntity)[EntityWithAccessionedSequence:192209] TGS1 [cytosol] [Homo sapiens]
[EntityWithAccessionedSequence:400176] TGS1 [nucleoplasm] [Homo sapiens]
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No pathways have been reviewed or authored by UniProt:Q96RS0 TGS1 (192207)