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Details on Person UniProt:Q13535-1 ATR

Class:IdReferenceIsoform:176167
_chainChangeLogchain:1-2644 added on Fri February 6 2015
_displayNameUniProt:Q13535-1 ATR
_timestamp2025-02-21 19:15:44
chainchain:1-2644
checksum11BC22297FB9A802
commentFUNCTION Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor (PubMed:10597277, PubMed:10608806, PubMed:10859164, PubMed:11721054, PubMed:12791985, PubMed:12814551, PubMed:14657349, PubMed:14729973, PubMed:14742437, PubMed:15210935, PubMed:15496423, PubMed:16260606, PubMed:21144835, PubMed:21777809, PubMed:23273981, PubMed:25083873, PubMed:27723717, PubMed:27723720, PubMed:30139873, PubMed:33848395, PubMed:37788673, PubMed:37832547, PubMed:9427750, PubMed:9636169). Recognizes the substrate consensus sequence [ST]-Q (PubMed:10597277, PubMed:10608806, PubMed:10859164, PubMed:11721054, PubMed:12791985, PubMed:12814551, PubMed:14657349, PubMed:14729973, PubMed:14742437, PubMed:15210935, PubMed:15496423, PubMed:16260606, PubMed:21144835, PubMed:23273981, PubMed:27723717, PubMed:27723720, PubMed:33848395, PubMed:9427750, PubMed:9636169). Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RBBP8, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis (PubMed:11114888, PubMed:11418864, PubMed:11865061, PubMed:21777809, PubMed:23273981, PubMed:25083873, PubMed:9925639). Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism (PubMed:11673449). Required for FANCD2 ubiquitination (PubMed:15314022). Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication (PubMed:12526805). Acts as a regulator of the S-G2 transition by restricting the activity of CDK1 during S-phase to prevent premature entry into G2 (PubMed:30139873). Acts as a regulator of the nuclear envelope integrity in response to DNA damage and stress (PubMed:25083873, PubMed:37788673, PubMed:37832547). Acts as a mechanical stress sensor at the nuclear envelope: relocalizes to the nuclear envelope in response to mechanical stress and mediates a checkpoint via phosphorylation of CHEK1 (PubMed:25083873). Also promotes nuclear envelope rupture in response to DNA damage by mediating phosphorylation of LMNA at 'Ser-282', leading to lamin disassembly (PubMed:37832547). Involved in the inflammatory response to genome instability and double-stranded DNA breaks: acts by localizing to micronuclei arising from genome instability and catalyzing phosphorylation of LMNA at 'Ser-395', priming LMNA for subsequent phosphorylation by CDK1 and micronuclei envelope rupture (PubMed:37788673). The rupture of micronuclear envelope triggers the cGAS-STING pathway thereby activating the type I interferon response and innate immunity (PubMed:37788673). Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex (By similarity).CATALYTIC ACTIVITY L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)CATALYTIC ACTIVITY L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)COFACTOR Serine/threonine-protein kinase activity is directly stimulated by TOPBP1 (PubMed:16530042, PubMed:21777809). ATR kinase activity is also directly activated by ETAA1, independently of TOPBP1 (PubMed:27723717, PubMed:27723720, PubMed:30139873). Activated by DNA and inhibited by BCR-ABL oncogene (PubMed:10597277). Slightly activated by ATRIP (PubMed:14729973). Inhibited by caffeine, wortmannin and LY294002 (PubMed:9766667).SUBUNIT Forms a heterodimer with ATRIP, forming the ATR-ATRIP complex (PubMed:11721054, PubMed:12791985, PubMed:14729973, PubMed:15758953, PubMed:21777809). Present in a complex containing ATRIP and RPA-coated single-stranded DNA (PubMed:12791985, PubMed:14729973). Binds to DNA, and to UV-damaged DNA with higher affinity (PubMed:12791985). Interacts with MSH2 and HDAC2 (PubMed:14657349). Present in a complex containing CHD4 and HDAC2 (PubMed:10545197). Interacts with EEF1E1, the interaction is enhanced by UV irradiation (PubMed:15680327). Interacts with CLSPN and CEP164 (PubMed:12766152, PubMed:18283122). Interacts with TELO2 and TTI1 (PubMed:20427287, PubMed:20801936, PubMed:20810650). Interacts with BCR-ABL after genotoxic stress (PubMed:15050919). Interacts with UHRF2; this interaction promotes ATR activation (PubMed:33848395). Interacts (when phosphorylated) with TOPBP1; interaction takes place when ATR is autophosphorylated at Thr-1989, leading to ATR activation by TOPBP1 (PubMed:21705319, PubMed:21777809).INTERACTION Depending on the cell type, it can also be found in PML nuclear bodies (PubMed:12814551). Recruited to chromatin during S-phase (PubMed:14871897). Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling (PubMed:27723720). Relocalizes to the nuclear envelope in response to mechanical stress or DNA damage (PubMed:25083873, PubMed:37832547). Also localizes to the micronuclear envelope in response to response to genome instability (PubMed:37788673).ALTERNATIVE PRODUCTS Ubiquitous, with highest expression in testis.TISSUE SPECIFICITY Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.PTM Phosphorylated (PubMed:21144835). Autophosphorylation at Thr-1989 in response to DNA damage promotes interaction with TOPBP1 and activation of ATR (PubMed:21705319, PubMed:21777809).DISEASE The disease is caused by variants affecting the gene represented in this entry.DISEASE The disease is caused by variants affecting the gene represented in this entry.SIMILARITY Belongs to the PI3/PI4-kinase family. ATM subfamily.
created[InstanceEdit:176246] D'Eustachio, P, 2006-03-03 16:44:08
descriptionrecommendedName: Serine/threonine-protein kinase ATR ecNumber evidence="40 41 45 46"2.7.11.1 alternativeName: Ataxia telangiectasia and Rad3-related protein alternativeName: FRAP-related protein 1
geneNameATR
FRP1
identifierQ13535
isoformParent
isSequenceChangedFALSE
keyword3D-structure
Alternative splicing
ATP-binding
Chromosome
Disease variant
DNA damage
DNA repair
DNA-binding
Dwarfism
Intellectual disability
Kinase
Manganese
Nucleotide-binding
Nucleus
Phosphoprotein
Proteomics identification
Reference proteome
Repeat
Serine/threonine-protein kinase
Transferase
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9862192] Weiser, Joel, 2024-02-26
[InstanceEdit:9909836] Weiser, Joel, 2024-05-14
[InstanceEdit:9917590] Weiser, Joel, 2024-08-09
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
nameATR
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:6797735] ENSEMBL:ENSG00000175054 ATR [Homo sapiens]
secondaryIdentifierATR_HUMAN
Q59HB2
Q7KYL3
Q93051
Q9BXK4
sequenceLength2644
species[Species:48887] Homo sapiens
variantIdentifierQ13535-1
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No pathways have been reviewed or authored by UniProt:Q13535-1 ATR (176167)