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WEE1, a nuclear kinase, phosphorylates cyclin B1:Cdc2 (CC...

Class:IdSummation:170201
_displayName

WEE1, a nuclear kinase, phosphorylates cyclin B1:Cdc2 (CC...

_timestamp2024-11-27 18:34:02
created[InstanceEdit:170184] Matthews, L, 2005-12-28 10:02:46
literatureReference[LiteratureReference:170219] Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine kinase
[LiteratureReference:8981828] Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic cyclins
[LiteratureReference:8981832] Human Wee1 kinase inhibits cell division by phosphorylating p34cdc2 exclusively on Tyr15
[LiteratureReference:77500] Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle
[LiteratureReference:9929418] Coupling of T161 and T14 phosphorylations protects cyclin B-CDK1 from premature activation
[LiteratureReference:9928910] Quantitative reconstitution of mitotic CDK1 activation in somatic cell extracts
modified[InstanceEdit:170262] Matthews, L, 2006-01-04 01:10:23
[InstanceEdit:8981831] Orlic-Milacic, Marija, 2017-03-14
[InstanceEdit:8981834] Orlic-Milacic, Marija, 2017-03-14
[InstanceEdit:9929423] Orlic-Milacic, Marija, 2024-11-25
[InstanceEdit:9929678] Orlic-Milacic, Marija, 2024-11-27
text

WEE1, a nuclear kinase, phosphorylates cyclin B1:Cdc2 (CCNB1:CDK1) on tyrosine 15 (Y15), inactivating the complex (Parker and Piwnica-Worms 1992, McGowan and Russell 1993, Watanabe et al., 1995). The complex of cyclin B2 and Cdc2 (CCNB2:CDK1) is also phosphorylated on Y15 (Galaktionov and Beach 1991). Two-dimensional gel electrophoresis of protein extracts of cycling human cells has shown that CDK1 triple phosphorylated at T14, Y15, and T161 is the most abundant form of CDK1 that co-immunoprecipitates with CCNA2 or CCNB1, and Y15 phosphorylation of CDK1 was confirmed to be WEE1-dependent (Coulonval et al. 2011).

CCNA:CDK1-mediated phosphorylation of WEE1 inhibiting WEE1 kinase activity and facilitates the activation of CCNB:CDK1 complexes (Deibler and Kirschner 2010).

(summation)[Reaction:170070] Wee1-mediated phosphorylation of Cyclin B1:phospho-Cdc2 complexes [Homo sapiens]
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WEE1, a nuclear kinase, phosphorylates cyclin B1:Cdc2 (CC... (170201)

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