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Details on Person UniProt:P03348 gag
| Class:Id | ReferenceGeneProduct:165253 |
|---|---|
| _chainChangeLog | initiator methionine:1 added on Fri February 6 2015;chain:2-512 added on Fri February 6 2015;chain:2-132 added on Fri February 6 2015;chain:133-363 added on Fri February 6 2015;peptide:364-377 added on Fri February 6 2015;chain:378-432 added on Fri February 6 2015;peptide:433-448 added on Fri February 6 2015;chain:449-512 added on Fri February 6 2015;initiator methionine:1 for 165253 removed on Fri Nov 03 2023;initiator methionine: for 165253 added on Fri Nov 03 2023;initiator methionine: for 165253 removed on Fri Aug 15 2025;initiator methionine:1 for 165253 added on Fri Aug 15 2025 |
| _displayName | UniProt:P03348 gag |
| _timestamp | 2026-02-20 21:59:23 |
| chain | initiator methionine:1 chain:2-512 chain:2-132 chain:133-363 peptide:364-377 chain:378-432 peptide:433-448 chain:449-512 |
| checksum | 9103D701A843466F |
| comment | FUNCTION Mediates, with Gag-Pol polyprotein, the essential events in virion assembly, including binding the plasma membrane, making the protein-protein interactions necessary to create spherical particles, recruiting the viral Env proteins, and packaging the genomic RNA via direct interactions with the RNA packaging sequence (Psi).FUNCTION Targets the polyprotein to the plasma membrane via a multipartite membrane-binding signal, that includes its myristoylated N-terminus (By similarity). Matrix protein is part of the pre-integration complex. Implicated in the release from host cell mediated by Vpu. Binds to RNA (By similarity).FUNCTION Forms the conical core that encapsulates the genomic RNA-nucleocapsid complex in the virion. Most core are conical, with only 7% tubular. The core is constituted by capsid protein hexamer subunits. The core is disassembled soon after virion entry (By similarity). The capsid promotes immune invasion by cloaking viral DNA from CGAS detection (By similarity). Host restriction factors such as TRIM5-alpha or TRIMCyp bind retroviral capsids and cause premature capsid disassembly, leading to blocks in reverse transcription. Capsid restriction by TRIM5 is one of the factors which restricts HIV-1 to the human species. Host PIN1 apparently facilitates the virion uncoating (By similarity). On the other hand, interactions with PDZD8 or CYPA stabilize the capsid (By similarity).FUNCTION Encapsulates and protects viral dimeric unspliced genomic RNA (gRNA). Binds these RNAs through its zinc fingers. Acts as a nucleic acid chaperone which is involved in rearangement of nucleic acid secondary structure during gRNA retrotranscription. Also facilitates template switch leading to recombination. As part of the polyprotein, participates in gRNA dimerization, packaging, tRNA incorporation and virion assembly.FUNCTION Plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1.SUBUNIT Homotrimer; further assembles as hexamers of trimers. Oligomerization possibly creates a central hole into which the cytoplasmic tail of the gp41 envelope protein may be inserted. Interacts with host TRIM22; this interaction seems to disrupt proper trafficking of Gag polyprotein and may interfere with budding. Interacts with host PDZD8. When ubiquitinated, interacts (via p6-gag domain) with host PACSIN2; this interaction allows PACSIN2 recruitment to viral assembly sites and its subsequent incorporation into virions. Interacts with MOV10 (By similarity).SUBUNIT Homotrimer; further assembles as hexamers of trimers. Interacts with gp41 (via C-terminus). Interacts with host CALM1; this interaction induces a conformational change in the Matrix protein, triggering exposure of the myristate group. Interacts with host AP3D1; this interaction allows the polyprotein trafficking to multivesicular bodies during virus assembly. Part of the pre-integration complex (PIC) which is composed of viral genome, matrix protein, Vpr and integrase.SUBUNIT Homodimer; the homodimer further multimerizes as homohexamers or homopentamers (By similarity). Interacts with host NUP98 (By similarity). Interacts with host PPIA/CYPA; this interaction stabilizes the capsid (By similarity). Interacts with host NUP153 (By similarity). Interacts with host PDZD8; this interaction stabilizes the capsid. Interacts with host TRIM5; this interaction destabilizes the capsid (By similarity). Interacts with host CPSF6 (By similarity). Interacts with host NONO; the interaction is weak (By similarity).SUBUNIT Interacts with host NUP98.SUBUNIT Interacts with Vpr; this interaction allows Vpr incorporation into the virion (PubMed:7474102). Interacts with host TSG101 (By similarity). Interacts with host PDCD6IP/AIP1 (By similarity).INTERACTION These locations are probably linked to virus assembly sites. The main location is the cell membrane, but under some circumstances, late endosomal compartments can serve as productive sites for virion assembly.SUBCELLULAR LOCATION Translation results in the formation of the Gag polyprotein most of the time. Ribosomal frameshifting at the gag-pol genes boundary occurs at low frequency and produces the Gag-Pol polyprotein. This strategy of translation probably allows the virus to modulate the quantity of each viral protein. Maintenance of a correct Gag to Gag-Pol ratio is essential for RNA dimerization and viral infectivity.DOMAIN Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. p6-gag contains two L domains: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 and a LYPX(n)L motif which interacts with PDCD6IP/AIP1.PTM Gag-Pol polyprotein: Specific enzymatic cleavages by the viral protease yield mature proteins.PTM Tyrosine phosphorylated presumably in the virion by a host kinase. Phosphorylation is apparently not a major regulator of membrane association.PTM Phosphorylated possibly by host MAPK1; this phosphorylation is necessary for Pin1-mediated virion uncoating.PTM Methylated by host PRMT6, impairing its function by reducing RNA annealing and the initiation of reverse transcription.MISCELLANEOUS HIV-1 lineages are divided in three main groups, M (for Major), O (for Outlier), and N (for New, or Non-M, Non-O). The vast majority of strains found worldwide belong to the group M. Group O seems to be endemic to and largely confined to Cameroon and neighboring countries in West Central Africa, where these viruses represent a small minority of HIV-1 strains. The group N is represented by a limited number of isolates from Cameroonian persons. The group M is further subdivided in 9 clades or subtypes (A to D, F to H, J and K).MISCELLANEOUS Produced by conventional translation.SIMILARITY Belongs to the primate lentivirus group gag polyprotein family. |
| created | [InstanceEdit:165206] Schmidt, EE, 2005-08-03 04:18:22 |
| description | recommendedName: Gag polyprotein alternativeName: Pr55Gag component recommendedName: Matrix protein p17 shortName: MA /component component recommendedName: Capsid protein p24 shortName: CA /component component recommendedName: fullName evidence="5"Spacer peptide 1 shortName: SP1 alternativeName: p2 /component component recommendedName: Nucleocapsid protein p7 shortName: NC /component component recommendedName: fullName evidence="5"Spacer peptide 2 shortName: SP2 alternativeName: p1 /component component recommendedName: p6-gag /component |
| geneName | gag |
| identifier | P03348 |
| isSequenceChanged | FALSE |
| keyword | AIDS Capsid protein Host cell membrane Host cytoplasm Host endosome Host membrane Host nucleus Host-virus interaction Lipoprotein Membrane Metal-binding Methylation Myristate Phosphoprotein Repeat Ribosomal frameshifting RNA-binding Viral budding Viral budding via the host ESCRT complexes Viral nucleoprotein Viral release from host cell Virion Zinc Zinc-finger |
| modified | [InstanceEdit:217385] Schmidt, EE, 2008-03-27 06:23:53 [InstanceEdit:354386] Schmidt, EE, 2008-06-18 04:45:12 [InstanceEdit:384350] Kanapin, AA, 2008-11-26 14:00:39 [InstanceEdit:392885] Kanapin, AA, 2009-03-09 12:07:18 [InstanceEdit:400710] Schmidt, EE, 2009-03-25 05:33:35 [InstanceEdit:423310] Kanapin, AA [InstanceEdit:435478] Kanapin, AA [InstanceEdit:435871] Kanapin, AA [InstanceEdit:447347] Kanapin, AA [InstanceEdit:525883] Kanapin, AA [InstanceEdit:613449] Kanapin, AA [InstanceEdit:797602] Kanapin, AA [InstanceEdit:937368] Yung, CK [InstanceEdit:1042053] Yung, CK [InstanceEdit:1220657] Yung, CK [InstanceEdit:1300696] Yung, CK [InstanceEdit:1301627] Yung, CK [InstanceEdit:1551960] Weiser, JD [InstanceEdit:1995863] Weiser, JD [InstanceEdit:2132304] Weiser, JD [InstanceEdit:2265580] Weiser, JD [InstanceEdit:2455454] Weiser, JD [InstanceEdit:5433710] Weiser, JD [InstanceEdit:5618415] Weiser, JD [InstanceEdit:5634237] Weiser, JD [InstanceEdit:5673015] Weiser, JD [InstanceEdit:5691543] Weiser, JD [InstanceEdit:8964659] Weiser, JD [InstanceEdit:9027688] Weiser, JD [InstanceEdit:9037114] Weiser, JD [InstanceEdit:9627708] Weiser, JD [InstanceEdit:9637257] Weiser, JD [InstanceEdit:9657908] Weiser, JD [InstanceEdit:9676415] Weiser, JD [InstanceEdit:9688885] Weiser, JD [InstanceEdit:9706439] Weiser, JD [InstanceEdit:9715482] Weiser, JD [InstanceEdit:9852000] Weiser, Joel, 2023-11-03 [InstanceEdit:9862192] Weiser, Joel, 2024-02-26 [InstanceEdit:9963647] Weiser, Joel, 2025-08-15 [InstanceEdit:9983091] Weiser, Joel, 2026-02-20 |
| name | gag |
| referenceDatabase | [ReferenceDatabase:2] UniProt |
| secondaryIdentifier | GAG_HV1BR |
| sequenceLength | 512 |
| species | [Species:164493] Human immunodeficiency virus 1 |
| (isoformParent) | [ReferenceIsoform:402311] UniProt:P03348-1 gag [Human immunodeficiency virus 1] |
| (referenceEntity) | [EntityWithAccessionedSequence:173135] GAG (P03348) protein [Human immunodeficiency virus 1] [EntityWithAccessionedSequence:173304] NC (P03348) protein [cytosol] [Human immunodeficiency virus 1] [EntityWithAccessionedSequence:173306] CA (P03348) protein [cytosol] [Human immunodeficiency virus 1] [EntityWithAccessionedSequence:173334] MA (P03348) protein [cytosol] [Human immunodeficiency virus 1] [EntityWithAccessionedSequence:173413] p6 (P03348) protein [cytosol] [Human immunodeficiency virus 1] [EntityWithAccessionedSequence:174995] MA (P03348) protein [Human immunodeficiency virus 1] [EntityWithAccessionedSequence:175053] p6 [nucleoplasm] [Human immunodeficiency virus 1] [EntityWithAccessionedSequence:175061] p6 (P03348) protein [Human immunodeficiency virus 1] [EntityWithAccessionedSequence:175142] CA (P03348) protein [Human immunodeficiency virus 1] [EntityWithAccessionedSequence:175568] MA (P03348) protein [nucleoplasm] [Human immunodeficiency virus 1] |
| (referenceSequence) | [ModifiedResidue:184256] N-myristoylglycine at 1 |
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No pathways have been reviewed or authored by UniProt:P03348 gag (165253)
