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Details on Person UniProt:Q9ZQ19 RPA2A

Class:IdReferenceGeneProduct:1601038
_chainChangeLogchain:1-279 for 1601038 added on Fri Nov 03 2023
_displayNameUniProt:Q9ZQ19 RPA2A
_timestamp2023-11-03 18:38:37
chainchain:1-279
checksum3DD5B70FE6FC5736
commentFUNCTION Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Required fo cell division in meristems. Involved in the maintenance of transcriptional epigenetic gene silencing (TGS) at specific loci (including some transposons) by regulating histone H3 acetylation, 'Lys-4' and 'Lys-9' methylation.SUBUNIT Heterotrimer of RPA1, RPA2 and RPA3 (canonical replication protein A complex) (By similarity). Interacts with ROS1. Binds to ASE1/At3g02920, PDX2, At5g62350, RPA1A/At2g06510, ARF1/At1g10630, At4g18590 and At3g52630.SUBCELLULAR LOCATION Redistributes to discrete nuclear foci upon DNA damage.TISSUE SPECIFICITY Strongly expressed in shoot and root meristems. Present in seedlings, roots, leaves, siliques and flowers.DEVELOPMENTAL STAGE Accumulates essentially in meristematic active tissues. In seedlings, expressed in primary roots, shoot apical meristems (SAMs), cotyledons, and vascular tissues. Later observed in lateral root primordia, root tips, SAMs and young leaves.PTM Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). In response to DNA damage, recruited to DNA-repair nuclear foci, as a hypophosphorylated form (By similarity).DISRUPTION PHENOTYPE Affected cell division in meristems but normal final cell sizes. Earlier flowering and smaller plant size. Enhanced sensitivity to methyl methanesulfonate (MMS), a genotoxic agent that damages DNA bases. Enhanced expression of some transposons. When associated with ROS1 disruption, restored silencing of some genes associated with increased histone H3 acetylation and histone H3 'Lys-4' methylation (H3K4me2), but decreased histone H3 'Lys-9' methylation (H3K9me2) in their promoter.SIMILARITY Belongs to the replication factor A protein 2 family.
created[InstanceEdit:1598120] Couchman, M, 2007-07-02
crossReference[DatabaseIdentifier:1600451] ATIDB:At2g24490
[DatabaseIdentifier:1600452] TAIR:At2g24490
[DatabaseIdentifier:1600453] MIPS:At2g24490
[DatabaseIdentifier:1600454] NCBI Entrez Gene:At2g24490
[DatabaseIdentifier:1600455] TIGR:At2g24490
descriptionrecommendedName: Replication protein A 32 kDa subunit A shortName: AtRPA32A shortName: RP-A p32 A alternativeName: DNA replication protein A2 subunit A alternativeName: Protein SUPPRESSOR OF ROS1 alternativeName: Replication factor A protein 2 A shortName: AtRPA2 A shortName: RF-A protein 2 A alternativeName: Replicon protein A2 A
geneNameRPA2A
ROR1
RPA32A
At2g24490
T28I24.22
identifierQ9ZQ19
isSequenceChangedFALSE
keywordDNA damage
DNA recombination
DNA repair
DNA replication
DNA-binding
Nucleus
Phosphoprotein
Reference proteome
modified[InstanceEdit:1598234] Couchman, MS
[InstanceEdit:1601927] Preece, J, 2011-09-12
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
nameRPA2A
otherIdentifierQ9ZQ19_ARATH
Q9ZQ19
265695_at
AT2G24490
referenceDatabase[ReferenceDatabase:2] UniProt
secondaryIdentifierRFA2A_ARATH
sequenceLength279
species[Species:170905] Arabidopsis thaliana
(referenceEntity)[EntityWithAccessionedSequence:1599251] RPA2A [Arabidopsis thaliana]
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No pathways have been reviewed or authored by UniProt:Q9ZQ19 RPA2A (1601038)