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Details on Person UniProt:P24100 CDKA-1

Class:IdReferenceGeneProduct:1600912
_chainChangeLogchain:1-294 added on Fri February 6 2015
_displayNameUniProt:P24100 CDKA-1
_timestamp2025-02-21 18:34:55
chainchain:1-294
checksumB5FAE55FA9EC366E
commentFUNCTION Involved in the control of the cell cycle. Essential for both G1/S and G2/M (mitosis) phase transitions. Functions in cell morphogenesis as well as cell proliferation. Required for cell division (entry into mitosis) of the generative cell in male gametogenesis. Required to trigger guard mother cells (GMC) symmetric divisions at the late stage of stomatal development, probably via the regulation of G1 to S transition in the cell cycle. Required for the function of SPCH in entering the stomatal lineage (PubMed:25680231). Promotes divisions in the guard cells (GCs) after the guard mother cells (GMC) symmetric division when in the presence of CYCD3-2 via the phosphorylation of SPCH (PubMed:24687979, PubMed:25680231).CATALYTIC ACTIVITY L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)CATALYTIC ACTIVITY L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)CATALYTIC ACTIVITY [DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)ACTIVITY REGULATION CDK kinase activated by CDKF-1. CDK kinase activity inhibited by KRP1/ICK1, KRP2/ICK2, KRP3/ICK6, KRP4/ICK7, KRP5/ICK3, KRP6/ICK4 and KRP7/ICK5. Down-regulated by phosphorylation by WEE1.SUBUNIT Interacts with CDT1A, CYCA2-3, CYCD2-1, CYCD3-1, CYCD4-1, CYCD4-2, CYCH1-1, CYCU1-1, CYCU2-1, CYCU2-2, CYCU3-1, CYCU4-1, CYCU4-2, CYCU4-3, CKS1, KRP2/ICK2, KRP3/ICK6, KRP4/ICK7, KRP6/ICK4, KRP7/ICK5, and C-terminal domain of KRP1/ICK1. Interacts with WEE1 and TIF4A-1/EIF4A-1. Interacts with PAS2; when phosphorylated at Tyr-15. Interacts with SMR3, SMR4, SMR5, SMR6, SMR8 and At4g14310. Binds to CYCD3-2 (PubMed:24687979). Component of a DREAM-like complex which modulates a variety of developmentally regulated genes and of the mitotic genes in proliferating and differentiated cells. Interacts with MYB3R3 at later and with MYB3R4 at earlier stages of leaf development (PubMed:26069325). May interact with SPCH (PubMed:25680231).INTERACTION Mainly cytoplasmic. Nuclear distribution increases after binding to ICK1/KRP1.TISSUE SPECIFICITY Expressed in roots, stems, flowers and siliques.DEVELOPMENTAL STAGE Expressed throughout the cell cycle. Expressed in actively dividing cells: root and shoot apical meristems, leaf primordia and emerging lateral root meristem. Expressed in light-grown seedlings from 1 up to 7 days after germination with a peak at 2 and 3 days.INDUCTION By nematode infection in roots. Down-regulated by salt stress in root meristem and replication blocking agents (hydroxyurea and aphidicolin).PTM Phosphorylated at Tyr-15 by WEE1. Phosphorylation at Thr-161 is important for the kinase activity and substrate binding. Binding to the anti-phosphatase PAS2 prevents dephosphorylation.DISRUPTION PHENOTYPE Plants display lethal male gametophyte (PubMed:16460514, PubMed:25680231). Impaired stomata formation with arrested guard mother cells (GMC) divisions (PubMed:25680231). Impaired last mitotic division in the male gametophyte, leading to 50 percent of pollen with two gametes (PubMed:25680231). The double mutant flp-1 myb88 displays an enhanced stomatal phenotype with more and larger stomatal clusters. Triple mutants cdka;1 flp-1 myb88 don't have guard cells stacks but accumulates sGCs (PubMed:24687979).SIMILARITY Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
created[InstanceEdit:1598031] Couchman, M, 2007-06-12
crossReference[DatabaseIdentifier:1599759] ATIDB:AT3G48750
[DatabaseIdentifier:1599757] TAIR:AT3G48750
[DatabaseIdentifier:1600109] MIPS:AT3G48750
[DatabaseIdentifier:1600110] NCBI Entrez Gene:AT3G48750
[DatabaseIdentifier:1600109] MIPS:AT3G48750
[DatabaseIdentifier:1600110] NCBI Entrez Gene:AT3G48750
[DatabaseIdentifier:1600111] TIGR:AT3G48750
[DatabaseIdentifier:1600111] TIGR:AT3G48750
descriptionrecommendedName: fullName evidence="46"Cyclin-dependent kinase A-1 shortName evidence="39"CDKA;1 ecNumber evidence="19 27"2.7.11.22 ecNumber evidence="19 27"2.7.11.23 alternativeName: fullName evidence="40 43"Cell division control protein 2 homolog A shortName evidence="44"CDC2aAt
geneNameCDKA-1
CDC2
CDC2A
At3g48750
T21J18.20
identifierP24100
isSequenceChangedFALSE
keywordATP-binding
Cell cycle
Cell division
Cytoplasm
Kinase
Mitosis
Nucleotide-binding
Nucleus
Phosphoprotein
Reference proteome
Serine/threonine-protein kinase
Transferase
modified[InstanceEdit:1598234] Couchman, MS
[InstanceEdit:1601927] Preece, J, 2011-09-12
[InstanceEdit:1995863] Weiser, JD
[InstanceEdit:2132304] Weiser, JD
[InstanceEdit:2265580] Weiser, JD
[InstanceEdit:5433710] Weiser, JD
[InstanceEdit:5634237] Weiser, JD
[InstanceEdit:5673015] Weiser, JD
[InstanceEdit:9037114] Weiser, JD
[InstanceEdit:9627708] Weiser, JD
[InstanceEdit:9637257] Weiser, JD
[InstanceEdit:9676415] Weiser, JD
[InstanceEdit:9730071] Weiser, JD
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
nameCDKA-1
otherIdentifierAT3G48750
CDC2A_ARATH
CDC2A
Q29Q50_ARATH
Q29Q50
252337_at
Q8RX68_ARATH
Q8RX68
P24100
P24100_ARATH
referenceDatabase[ReferenceDatabase:2] UniProt
secondaryIdentifierCDKA1_ARATH
Q29Q50
Q8RX68
Q9M307
sequenceLength294
species[Species:170905] Arabidopsis thaliana
(referenceEntity)[EntityWithAccessionedSequence:1599062] CDKA;1 [nucleoplasm] [Arabidopsis thaliana]
[EntityWithAccessionedSequence:1599306] CDKA;1 [cytosol] [Arabidopsis thaliana]
[EntityWithAccessionedSequence:1599429] CDKA;1 [preprophase band] [Arabidopsis thaliana]
[EntityWithAccessionedSequence:1599441] CDKA;1-phosphorylated (Thr160) [nucleoplasm] [Arabidopsis thaliana]
(referenceSequence)[ModifiedResidue:1600813] phosphate [nucleoplasm] on ChEBI:32835 threonine residue 160 of UNIPROT:CDC2A_ARATH Cell division control protein 2 homolog A
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