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Details on Person UniProt:Q9M4A2 SPO11-1

Class:IdReferenceGeneProduct:1600841
_chainChangeLogchain:1-362 added on Sat February 7 2015
_displayNameUniProt:Q9M4A2 SPO11-1
_timestamp2024-08-09 19:43:55
chainchain:1-362
checksumAE82942C4F456770
commentFUNCTION Component of a topoisomerase 6 complex specifically required for meiotic recombination (PubMed:11157765, PubMed:17018031, PubMed:17965269, PubMed:26917763). Together with MTOPVIB, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (PubMed:19763177, PubMed:26917763). The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles (PubMed:11157765, PubMed:17018031, PubMed:17965269, PubMed:26917763).CATALYTIC ACTIVITY ATP-dependent breakage, passage and rejoining of double-stranded DNA.COFACTOR Heterotetramer of 2 SPO11 (SPO11-1 and/or SPO11-2) and 2 MTOPVIB chains (Probable). Interacts with MTOPVIB (PubMed:26917763, PubMed:28855712). May form a heterodimer with SPO11-2. Interacts with PRD1 (PubMed:17762870, PubMed:28855712). Does not interact with TOP6B (PubMed:11410368).INTERACTION A number of isoforms are produced. According to EST sequences.TISSUE SPECIFICITY Expressed in shoots, young seedlings, flowers and reproductive tissues. Not found in roots or rosette leaves.DEVELOPMENTAL STAGE Accumulates in early G2 phase and then disappears over a period of between 1 and 5 hours post-S phase.DISRUPTION PHENOTYPE Plants show a semi-sterile phenotype and a drastic decrease of meiotic recombination, indicating that SPO11-2 and SPO11-3 are not functionally redundant. SPO11-1 and SPO11-2 are both required for double-strand breaks induction. Drastic decrease in chiasma formation at metaphase I associated with an absence of synapsis in prophase, due to the inability to make double-strand breaks (DSB) (PubMed:19763177).SIMILARITY Belongs to the TOP6A family.
created[InstanceEdit:1598157] Couchman, M, 2007-08-14
crossReference[DatabaseIdentifier:1599925] ATIDB:AT3G13170
[DatabaseIdentifier:1599926] TAIR:AT3G13170
[DatabaseIdentifier:1599927] MIPS:AT3G13170
[DatabaseIdentifier:1599928] TIGR:AT3G13170
descriptionrecommendedName: fullName evidence="13"Meiotic recombination protein SPO11-1 shortName evidence="13"AtSPO11-1 ecNumber evidence="14"5.6.2.2
geneNameSPO11-1
At3g13170
MJG19.19
identifierQ9M4A2
isSequenceChangedFALSE
keywordAlternative splicing
DNA-binding
Isomerase
Magnesium
Meiosis
Metal-binding
Nucleus
Reference proteome
Topoisomerase
modified[InstanceEdit:1598234] Couchman, MS
[InstanceEdit:1601927] Preece, J, 2011-09-12
[InstanceEdit:1995863] Weiser, JD
[InstanceEdit:2132304] Weiser, JD
[InstanceEdit:2265580] Weiser, JD
[InstanceEdit:5433710] Weiser, JD
[InstanceEdit:5673015] Weiser, JD
[InstanceEdit:8856987] Weiser, JD
[InstanceEdit:8934940] Weiser, JD
[InstanceEdit:9037114] Weiser, JD
[InstanceEdit:9637257] Weiser, JD
[InstanceEdit:9645038] Weiser, JD
[InstanceEdit:9657908] Weiser, JD
[InstanceEdit:9676415] Weiser, JD
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9862192] Weiser, Joel, 2024-02-26
[InstanceEdit:9917590] Weiser, Joel, 2024-08-09
nameSPO11-1
otherIdentifierAT3G13170
Q9M4A2_ARATH
Q9M4A2
257179_at
referenceDatabase[ReferenceDatabase:2] UniProt
secondaryIdentifierSPO11_ARATH
Q9C5S3
Q9LK56
sequenceLength362
species[Species:170905] Arabidopsis thaliana
(isoformParent)[ReferenceIsoform:8973084] UniProt:Q9M4A2-1 SPO11-1 [Arabidopsis thaliana]
(referenceEntity)[EntityWithAccessionedSequence:1599131] AtSPO11-1 [nucleoplasm] [Arabidopsis thaliana]
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