Reactome: A Curated Pathway Database
THIS SITE IS USED FOR CURATION AND TESTING
IT IS NOT STABLE, IS LINKED TO AN INCOMPLETE DATA SET, AND IS NOT MONITORED FOR PERFORMANCE. WE STRONGLY RECOMMEND THE USE OF OUR PUBLIC SITE

Query author contributions in Reactome

Reactome depends on collaboration between our curation team and outside experts to assemble and peer-review its pathway modules. The integration of ORCID within Reactome enables us to meet a key challenge with authoring, curating and reviewing biological information by incentivizing and crediting the external experts that contribute their expertise and time to the Reactome curation process. More information is available at ORCID and Reactome.

If you have an ORCID ID that is not listed on this page, please forward this information to us and we will update your Reactome pathway records.

Name Email address

Details on Person UniProt:O60264 SMARCA5

Class:IdReferenceGeneProduct:154105
_chainChangeLoginitiator methionine:1 added on Fri February 6 2015;chain:2-1052 added on Fri February 6 2015;initiator methionine:1 for 154105 removed on Fri Nov 03 2023;initiator methionine: for 154105 added on Fri Nov 03 2023;initiator methionine: for 154105 removed on Fri Aug 15 2025;initiator methionine:1 for 154105 added on Fri Aug 15 2025
_displayNameUniProt:O60264 SMARCA5
_timestamp2025-08-15 21:18:55
chaininitiator methionine:1
chain:2-1052
checksum6CC8CB25BAF7A876
commentFUNCTION ATPase that possesses intrinsic ATP-dependent nucleosome-remodeling activity (PubMed:12972596, PubMed:28801535). Catalytic subunit of ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair; this may require intact histone H4 tails (PubMed:10880450, PubMed:12198550, PubMed:12434153, PubMed:12972596, PubMed:23911928, PubMed:28801535). Within the ISWI chromatin-remodeling complexes, slides edge- and center-positioned histone octamers away from their original location on the DNA template (PubMed:28801535). Catalytic activity and histone octamer sliding propensity is regulated and determined by components of the ISWI chromatin-remodeling complexes (PubMed:28801535). The BAZ1A/ACF1-, BAZ1B/WSTF-, BAZ2A/TIP5- and BAZ2B-containing ISWI chromatin-remodeling complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template in an ATP-dependent manner (PubMed:14759371, PubMed:15543136, PubMed:28801535). The CECR2- and RSF1-containing ISWI chromatin-remodeling complexes do not have the ability to slide mononucleosomes to the center of a DNA template (PubMed:28801535). Binds to core histones together with RSF1, and is required for the assembly of regular nucleosome arrays by the RSF-5 ISWI chromatin-remodeling complex (PubMed:12972596). Involved in DNA replication and together with BAZ1A/ACF1 is required for replication of pericentric heterochromatin in S-phase (PubMed:12434153). Probably plays a role in repression of RNA polymerase I dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter (By similarity). Essential component of the WICH-5 ISWI chromatin-remodeling complex (also called the WICH complex), a chromatin-remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure (PubMed:11980720, PubMed:15543136). The WICH-5 ISWI chromatin-remodeling complex regulates the transcription of various genes, has a role in RNA polymerase I transcription (By similarity). Within the B-WICH complex has a role in RNA polymerase III transcription (PubMed:16603771). Mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes (By similarity). Essential component of NoRC-5 ISWI chromatin-remodeling complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing (By similarity).CATALYTIC ACTIVITY ATP + H2O = ADP + phosphate + H(+)SUBUNIT Component of the ACF-5 ISWI chromatin-remodeling complex (also called the ACF/WCRF complex) at least composed of SMARCA5/SNF2H and BAZ1A/ACF1, which regulates the spacing of histone octamers on the DNA template to facilitate access to DNA (PubMed:10880450, PubMed:12198550, PubMed:12434153, PubMed:28801535). Within the complex interacts with BAZ1A/ACF1; the interaction is direct and is required to slide nucleosomes from end to center positions on a DNA template in an ATP-dependent manner (PubMed:10880450, PubMed:12198550, PubMed:12434153, PubMed:28801535). Component of the CHRAC ISWI chromatin-remodeling complex at least composed of SMARCA5/SNF2H, BAZ1A/ACF1, CHRAC1 and POLE3; the complex preferentially binds DNA through the CHRAC1-POLE3 heterodimer and possesses ATP-dependent nucleosome-remodeling activity (PubMed:10880450, PubMed:12434153). Within the complex interacts with BAZ1A/ACF1; the interaction is direct and promotes the interaction with the POLE3-CHRAC1 heterodimer (PubMed:10880450, PubMed:12434153, PubMed:14759371). Within the complex interacts with the POLE3-CHRAC1 heterodimer; the interaction is direct and enhances nucleosome sliding activity by the SMARCA5/SNF2H and BAZ1A/ACF1 interaction (PubMed:10880450, PubMed:14759371). Neither POLE3 nor CHRAC1 enhances nucleosome sliding activity of the ACF-5 ISWI chromatin remodeling complex (PubMed:14759371). Component of the WICH-5 ISWI chromatin-remodeling complex (also called the WICH complex) at least composed of SMARCA5/SNF2H and BAZ1B/WSTF, which regulates the spacing of histone octamers on the DNA template to facilitate access to DNA (PubMed:11980720, PubMed:28801535). Within the complex interacts with BAZ1B/WSTF (PubMed:11980720, PubMed:15543136, PubMed:28801535). Component of the NoRC-5 ISWI chromatin-remodeling complex (also called the NoRC chromatin-remodeling complex) at least composed of SMARCA5/SNF2H and BAZ2A/TIP5; the complex suppresses rDNA transcription by a combination of nucleosome remodeling, histone deacetylation, and DNA methylation (PubMed:28801535). Within the complex interacts with BAZ2A/TIP5 (PubMed:28801535). Within the complex interacts with HDAC1 (By similarity). Component of the BRF-5 ISWI chromatin-remodeling complex at least composed of SMARCA5/SNF2H and BAZ2B (PubMed:28801535). Within the complex interacts with BAZ2B (PubMed:28801535). Component of the NURF-5 ISWI chromatin-remodeling complex at least composed of SMARCA5/SNF2H and BPTF (PubMed:28801535). Within the complex interacts with BPFT (PubMed:28801535). Component of the CERF-5 ISWI chromatin-remodeling complex at least composed of SMARCA5/SNF2H and CECR2 (PubMed:28801535). LUZP1 is detected as part of the CERF-5 complex in embryonic stem cells where it is involved in complex stabilization but is not detected in the complex in the testis (By similarity). Within the complex interacts with CECR2 (PubMed:28801535). Component of the RSF-5 ISWI chromatin-remodeling complex (also called the RSF complex) at least composed of SMARCA5/SNF2H and RSF1 (PubMed:12972596, PubMed:28801535). Within the complex interacts with RSF1 (PubMed:12972596, PubMed:28801535). Interacts with the cohesin complex component RAD21; the interaction is direct (PubMed:12198550). Interacts with the NuRD complex components HDAC2, RBBP4 and CHD4; the interactions are direct (PubMed:12198550). Interacts with PCNA (PubMed:15543136). Component of the B-WICH complex, at least composed of SMARCA5/SNF2H, BAZ1B/WSTF, SF3B1, DEK, MYO1C, ERCC6, MYBBP1A and DDX21 which positively regulates RNA polymerase III transcription (PubMed:16603771). Interacts with MYO1C (By similarity). Interacts with BEND3 (PubMed:26100909). Interacts with SIRT6; promoting recruitment to DNA damage sites (PubMed:23911928).SUBUNIT (Microbial infection) Interacts with JC virus small t antigen.SUBUNIT (Microbial infection) Interacts with Epstein Barr virus (EBV) lytic switch protein BZLF1; this interaction participates to the activation of early lytic viral genes by BZLF1.INTERACTION Localizes to mitotic chromosomes (PubMed:12972596). Co-localizes with RSF1 in the nucleus (PubMed:12972596). Co-localizes with PCNA at replication foci during S phase (PubMed:15543136). Co-localizes with BAZ1B/WSTF at replication foci during late-S phase (PubMed:15543136). Recruited to DNA damage sites following interaction with SIRT6 (PubMed:23911928).TISSUE SPECIFICITY Ubiquitously expressed.DEVELOPMENTAL STAGE Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy.SIMILARITY Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.CAUTION Like other proteins within the SNF2 family, they do not possess helicase activity but instead remodel chromatin via an ATP-dependent translocation mechanism.
created[InstanceEdit:143527] Schmidt, EE, 2004-11-12 07:45:10
descriptionrecommendedName: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 shortName evidence="23"SMARCA5 shortName: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5 ecNumber evidence="11 17"3.6.4.- alternativeName: Sucrose nonfermenting protein 2 homolog shortName evidence="20 21 22 23"hSNF2H
geneNameSMARCA5
SNF2H
WCRF135
identifierO60264
isSequenceChangedFALSE
keyword3D-structure
Acetylation
Chromatin regulator
Chromosome
Host-virus interaction
Hydrolase
Isopeptide bond
Nucleus
Phosphoprotein
Proteomics identification
Reference proteome
Repeat
Ubl conjugation
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9917590] Weiser, Joel, 2024-08-09
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
[InstanceEdit:9963647] Weiser, Joel, 2025-08-15
nameSMARCA5
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:8989220] ENSEMBL:ENSG00000153147 SMARCA5 [Homo sapiens]
secondaryIdentifierSMCA5_HUMAN
sequenceLength1052
species[Species:48887] Homo sapiens
(referenceEntity)[EntityWithAccessionedSequence:417140] SMARCA5 [nucleoplasm] [Homo sapiens]
[Change default viewing format]
No pathways have been reviewed or authored by UniProt:O60264 SMARCA5 (154105)
Follow Reactome