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Details on Person UniProt:Q9BZD2 SLC29A3

Class:IdReferenceGeneProduct:153494
_chainChangeLogchain:1-475 added on Fri February 6 2015
_displayNameUniProt:Q9BZD2 SLC29A3
_timestamp2024-11-03 20:11:57
chainchain:1-475
checksumDBF0918ECA6D5A70
commentFUNCTION Uniporter that mediates the facilitative transport of nucleoside across lysosomal and mitochondrial membranes (PubMed:15701636, PubMed:19164483, PubMed:20595384, PubMed:28729424). Functions as a non-electrogenic Na(+)-independent transporter (PubMed:15701636, PubMed:19164483, PubMed:28729424). Substrate transport is pH-dependent and enhanced under acidic condition, probably reflecting the location of the transporter in acidic intracellular compartments (PubMed:15701636, PubMed:19164483, PubMed:28729424). Proton is not a cotransporting ion but most likely change the ionization state of the transporter which dictates transport-permissible/impermissible conformation for nucleoside translocation (PubMed:28729424). May direct the nucleoside transport from lysosomes to cytosol or cytosol to mitochondria to facilitate the fundamental function of salvage synthesis of nucleic acids (PubMed:28729424). Involved in the transport of nucleosides (adenosine, guanosine, uridine, thymidine, cytidine and inosine) and deoxynucleosides (deoxyadenosine, deoxycytidine) (PubMed:15701636, PubMed:19164483, PubMed:20595384, PubMed:28729424). Also mediates transport of purine nucleobases (adenine, guanine) and pyrimidine nucleobases (uracil) (PubMed:15701636, PubMed:19164483). Also able to transport monoamine neurotransmitters dopamine, serotonin, noradrenaline and tyramine (PubMed:19164483). Capable of transporting ATP (PubMed:19164483). Mediates nucleoside export from lysosomes in macrophages, which regulates macrophage functions and numbers (By similarity).CATALYTIC ACTIVITY adenosine(in) = adenosine(out)CATALYTIC ACTIVITY guanosine(in) = guanosine(out)CATALYTIC ACTIVITY inosine(in) = inosine(out)CATALYTIC ACTIVITY uridine(out) = uridine(in)CATALYTIC ACTIVITY cytidine(in) = cytidine(out)CATALYTIC ACTIVITY thymidine(in) = thymidine(out)CATALYTIC ACTIVITY 2'-deoxyadenosine(in) = 2'-deoxyadenosine(out)CATALYTIC ACTIVITY 2'-deoxycytidine(in) = 2'-deoxycytidine(out)CATALYTIC ACTIVITY guanine(out) = guanine(in)CATALYTIC ACTIVITY uracil(in) = uracil(out)CATALYTIC ACTIVITY (R)-noradrenaline(out) = (R)-noradrenaline(in)CATALYTIC ACTIVITY dopamine(out) = dopamine(in)CATALYTIC ACTIVITY serotonin(out) = serotonin(in)CATALYTIC ACTIVITY tyramine(in) = tyramine(out)CATALYTIC ACTIVITY ATP(in) = ATP(out)BIOPHYSICOCHEMICAL PROPERTIES Optimum pH is 5.5 for adenosine transport (PubMed:15701636, PubMed:19164483, PubMed:28729424). No adenosine transport at pH 7.4 (PubMed:28729424).INTERACTION Observed in a punctate intracellular pattern showing partial colocalization with late endosomes/lysosomes (PubMed:15701636). Detected at the cell surface only in certain placental cells (PubMed:19164483).ALTERNATIVE PRODUCTS Widely expressed in both adult and fetal tissues (PubMed:15701636). Highest levels in placenta, uterus, ovary, spleen, lymph node and bone marrow (PubMed:15701636). Expressed in liver (PubMed:19164483). Lowest levels in brain and heart (PubMed:15701636). Expressed in macrophages (PubMed:22174130).DOMAIN Contains a N-terminal dileucine motif (DE)XXXL(LI) important for endosomal/lysosomal and mitochondrial subcellular localization.DISEASE The disease is caused by variants affecting the gene represented in this entry.MISCELLANEOUS Transports nucleoside analog drugs such as cladribine, cordycepin, tubercidin and idovudine (PubMed:15701636, PubMed:19164483). Also involved in the uptake of diabetes treatment medicine metformin, neurotoxin 1-methyl-4-phenylpyridinium (MPP(+)), and ribavirin (PubMed:19164483). Transport activity is insensitive to nanomolar concentrations of the inhibitors nitrobenzylmercaptopurine riboside, dipyridamole and dilazep, and inhibited by higher concentrations (PubMed:15701636). Does not transport hypoxanthine (PubMed:15701636, PubMed:19164483). A truncated version of SLC29A3/hENT3 in which the N-terminal 36 amino acids are deleted, enables cell-surface localization of an otherwise intracellular transporter, and is utilized to investigate the transporter activity (PubMed:15701636, PubMed:19164483, PubMed:20595384, PubMed:28729424).SIMILARITY Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.SEQUENCE CAUTION Truncated N-terminus.
created[InstanceEdit:143527] Schmidt, EE, 2004-11-12 07:45:10
descriptionrecommendedName: fullName evidence="24"Equilibrative nucleoside transporter 3 shortName evidence="24"hENT3 alternativeName: fullName evidence="25"Solute carrier family 29 member 3
geneNameSLC29A3
ENT3
UNQ717/PRO1380
identifierQ9BZD2
isSequenceChangedFALSE
keywordAlternative splicing
Cell membrane
Disease variant
Endosome
Glycoprotein
Lysosome
Membrane
Mitochondrion
Phosphoprotein
Proteomics identification
Reference proteome
Transmembrane
Transmembrane helix
Transport
modified[InstanceEdit:9836292] Weiser, Joel, 2023-05-25
[InstanceEdit:9841277] Weiser, Joel
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9926675] Weiser, Joel, 2024-11-03
nameSLC29A3
referenceDatabase[ReferenceDatabase:2] UniProt
referenceGene[ReferenceDNASequence:8988649] ENSEMBL:ENSG00000198246 SLC29A3 [Homo sapiens]
secondaryIdentifierS29A3_HUMAN
B2RB50
B4E2Z9
B7ZA37
Q0VAM9
Q5T465
Q7RTT8
Q8IVZ0
Q9BWI2
Q9NUS9
sequenceLength475
species[Species:48887] Homo sapiens
(isoformParent)[ReferenceIsoform:436056] UniProt:Q9BZD2-1 SLC29A3 [Homo sapiens]
[ReferenceIsoform:436057] UniProt:Q9BZD2-2 SLC29A3 [Homo sapiens]
(referenceEntity)[EntityWithAccessionedSequence:727733] SLC29A3 [lysosomal membrane] [Homo sapiens]
[EntityWithAccessionedSequence:5628740] SLC29A3 G427S [lysosomal membrane] [Homo sapiens]
[EntityWithAccessionedSequence:5628763] SLC29A3 R363Q [lysosomal membrane] [Homo sapiens]
[EntityWithAccessionedSequence:5628764] SLC29A3 M116R [lysosomal membrane] [Homo sapiens]
[EntityWithAccessionedSequence:5628793] SLC29A3 R363W [lysosomal membrane] [Homo sapiens]
[EntityWithAccessionedSequence:5628797] SLC29A3 L348Sfs*56 [lysosomal membrane] [Homo sapiens]
[EntityWithAccessionedSequence:5628819] SLC29A3 R386Q [lysosomal membrane] [Homo sapiens]
[EntityWithAccessionedSequence:9754911] SLC29A3 [mitochondrial outer membrane] [Homo sapiens]
(referenceSequence)[ReplacedResidue:5628737] L-arginine 386 replaced with L-glutamine
[ReplacedResidue:5628749] L-arginine 363 replaced with L-glutamine
[ReplacedResidue:5628753] glycine 427 replaced with L-serine
[FragmentReplacedModification:5628762] Replacement of residues 348 to 403 by SLPSSCTTLLTYVAGSSPPGSRCQGPIARRSQGSCSSGPASSPSSCSVTTSPAST
[ReplacedResidue:5628788] L-methionine 116 replaced with L-arginine
[ReplacedResidue:5628814] L-arginine 363 replaced with L-phenylalanine
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No pathways have been reviewed or authored by UniProt:Q9BZD2 SLC29A3 (153494)