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Details on Person UniProt:Q6ZD89 COMT

Class:IdReferenceGeneProduct:1159587
_chainChangeLogpropeptide:1-2 added on Sat February 7 2015;chain:3-368 added on Sat February 7 2015
_displayNameUniProt:Q6ZD89 COMT
_timestamp2025-02-21 19:30:36
chainpropeptide:1-2
chain:3-368
checksum2491B4FA7447558E
commentFUNCTION Methylates OH residues of flavonoid compounds. Can methylate eriodictyol, luteolin, quercetin and taxifolin (PubMed:16412485). Methylates caffeate to produce ferrulate (PubMed:21210840, Ref.16). Catalyzes the methylation of monolignols, the lignin precursors. Functions cooperatively with CAD2 in the culm internodes for the biosynthesis of monolignols. May be involved in lignin biosynthesis in leaves and roots (PubMed:21912859). Involved in syringyl lignin biosynthesis. Can function as 5-hydroxyconiferaldehyde O-methyltransferase in the biosynthetic pathway to syringyl lignin (Ref.16). Involved in melatonin biosynthesis. Can function as acetylserotonin O-methyltransferase. Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine) (PubMed:26276868).CATALYTIC ACTIVITY a 3'-hydroxyflavone + S-adenosyl-L-methionine = a 3'-methoxyflavone + S-adenosyl-L-homocysteine + H(+)CATALYTIC ACTIVITY N-acetylserotonin + S-adenosyl-L-methionine = melatonin + S-adenosyl-L-homocysteine + H(+)CATALYTIC ACTIVITY (E)-caffeate + S-adenosyl-L-methionine = (E)-ferulate + S-adenosyl-L-homocysteine + H(+)ACTIVITY REGULATION Acetylserotonin O-methyltransferase activity is inhibited by quercetin and caffeate.BIOPHYSICOCHEMICAL PROPERTIES Measured at pH 7.5 and 37 degrees Celsius for all experiments.PATHWAY Flavonoid metabolism; quercetin degradation.SUBUNIT Monomer.SUBCELLULAR LOCATION Expressed in roots and stems, and at lower levels in leaves.INDUCTION By probenazole (at protein level) (PubMed:17950386). Induced by UV-C (PubMed:24035516).SIMILARITY Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.CAUTION Was initially thought to constitute the naringenin 7-O-methyltransferase (NOMT), a methyltransferase involved in the biosynthesis of the sakuranetin, an inducible defense mechanism of O.sativa against pathogen attack (PubMed:10814825). However, it was later shown that it is not the case (PubMed:22493492).SEQUENCE CAUTION Truncated N-terminus.
created[InstanceEdit:1164949] Wu, Guanming, 2010-12-11
crossReference[DatabaseIdentifier:1141703] UniProtKB:A2YRE2
[DatabaseIdentifier:1145114] UniProtKB:Q19BJ6
[DatabaseIdentifier:1146574] UniProtKB:Q6ZD89
[DatabaseIdentifier:1147226] UniProtKB:A3BPT2
descriptionrecommendedName: fullName evidence="16"Flavone 3'-O-methyltransferase 1 shortName evidence="16"OsOMT1 ecNumber evidence="5"2.1.1.42 alternativeName: fullName evidence="16"Acetylserotonin O-methyltransferase COMT ecNumber evidence="11"2.1.1.4 alternativeName: fullName evidence="16"Caffeate O-methyltransferase 1 shortName evidence="15"OsCOMT1 ecNumber evidence="12"2.1.1.68 alternativeName: fullName evidence="14"OsCOMT alternativeName: fullName evidence="16"Quercetin 3'-O-methyltransferase 1
geneNameCOMT
ROMT-9
Os08g0157500
LOC_Os08g06100
OsJ_26105
P0438H08.29
identifierQ6ZD89
isSequenceChangedFALSE
keywordCytoplasm
Direct protein sequencing
Melatonin biosynthesis
Methyltransferase
Reference proteome
S-adenosyl-L-methionine
Transferase
modified[InstanceEdit:1251358] D'Eustachio, P, 2011-04-25
[InstanceEdit:1300696] Yung, CK
[InstanceEdit:1301627] Yung, CK
[InstanceEdit:1551960] Weiser, JD
[InstanceEdit:1995863] Weiser, JD
[InstanceEdit:2132304] Weiser, JD
[InstanceEdit:2265580] Weiser, JD
[InstanceEdit:2587579] Weiser, JD
[InstanceEdit:3445779] Weiser, JD
[InstanceEdit:5083144] Weiser, JD
[InstanceEdit:5433710] Weiser, JD
[InstanceEdit:5634237] Weiser, JD
[InstanceEdit:5673015] Weiser, JD
[InstanceEdit:8934940] Weiser, JD
[InstanceEdit:8944791] Weiser, JD
[InstanceEdit:8987656] Weiser, JD
[InstanceEdit:9037114] Weiser, JD
[InstanceEdit:9627708] Weiser, JD
[InstanceEdit:9637257] Weiser, JD
[InstanceEdit:9676415] Weiser, JD
[InstanceEdit:9852000] Weiser, Joel, 2023-11-03
[InstanceEdit:9939033] Weiser, Joel, 2025-02-21
nameCOMT
referenceDatabase[ReferenceDatabase:2] UniProt
secondaryIdentifierOMT1_ORYSJ
A0A0P0XBV5
A3BPT2
Q19BJ6
Q75W57
sequenceLength368
species[Species:1250427] Oryza sativa subsp. japonica
(referenceEntity)[EntityWithAccessionedSequence:1126515] O-methyltransferase (LOC_OS08G06100.1) [cytosol] [Oryza sativa subsp. japonica]
[EntityWithAccessionedSequence:9609608] ROMT-9 [cytosol] [Oryza sativa]
[EntityWithAccessionedSequence:9916171] OsOMT [cytosol] [Oryza sativa]
[EntityWithAccessionedSequence:9916312] OsOMT [nucleoplasm] [Oryza sativa]
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No pathways have been reviewed or authored by UniProt:Q6ZD89 COMT (1159587)